KEGG   ORTHOLOGY: K13310
Entry
K13310                      KO                                     

Name
desV, eryCI
Definition
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase [EC:2.6.1.106]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00797  dTDP-D-desosamine biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13310  desV, eryCI; dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
Enzymes [BR:ko01000]
 2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.106  dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
     K13310  desV, eryCI; dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
Other DBs
RN: R06426
Genes
SRN: A4G23_00481(fdtB_1)
SPAV: Spa2297_32850
SALJ: SMD11_1659
SEN: SACE_0734(eryCI)
ACTI: UA75_17820
ACAD: UA74_17285
AHG: AHOG_15460(fdtB2)
PLAB: C6361_24070
PLAT: C6W10_35925
AEY: CDG81_13130
AG: AAC68680(desV)
 » show all
Reference
  Authors
Burgie ES, Thoden JB, Holden HM
  Title
Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine.
  Journal
Protein Sci 16:887-96 (2007)
DOI:10.1110/ps.062711007
Reference
  Authors
Szu PH, He X, Zhao L, Liu HW
  Title
Biosynthesis of TDP-D-desosamine: identification of a strategy for C4 deoxygenation.
  Journal
Angew Chem Int Ed Engl 44:6742-6 (2005)
DOI:10.1002/anie.200501998
Reference
PMID:9770448
  Authors
Xue Y, Zhao L, Liu HW, Sherman DH
  Title
A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity.
  Journal
Proc Natl Acad Sci U S A 95:12111-6 (1998)
DOI:10.1073/pnas.95.21.12111
  Sequence

KEGG   ORTHOLOGY: K16436
Entry
K16436                      KO                                     

Name
evaB, megDII, angB, staI
Definition
dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase [EC:2.6.1.- 2.6.1.106]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01055  Biosynthesis of vancomycin group antibiotics
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00797  dTDP-D-desosamine biosynthesis
M00800  dTDP-L-megosamine biosynthesis
M00803  dTDP-D-angolosamine biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K16436  evaB, megDII, angB, staI; dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase
   01055 Biosynthesis of vancomycin group antibiotics
    K16436  evaB, megDII, angB, staI; dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase
Enzymes [BR:ko01000]
 2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.106  dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
     K16436  evaB, megDII, angB, staI; dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase
    2.6.1.-  
     K16436  evaB, megDII, angB, staI; dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase
Other DBs
RN: R06426 R06631 R08928
Genes
DTX: ATSB10_36020
PPF: Pput_3928
PVD: CFBP1590__4426(fdtB)
OME: OLMES_5572
BOK: DM82_922
BOC: BG90_13
BUU: WS70_10345
VEI: Veis_0694
CSER: CCO03_02175
OTO: ADJ79_07890
GUR: Gura_4276
GBM: Gbem_3307
GEO: Geob_0430
GEM: GM21_0941
DMA: DMR_07600
DBR: Deba_0354
BRAD: BF49_6200
RPE: RPE_1521
MAGN: WV31_12980
SGR: SGR_631
SLE: sle_09260(sle_09260)
SRN: A4G23_02284(fdtB_3)
NCA: Noca_4220
NDA: Ndas_4709
NAL: B005_1433
STRR: EKD16_15580(fdtB)
TCU: Tcur_2777
SACC: EYD13_16495(fdtB)
AOI: AORI_1500
AJA: AJAP_32020(dnrJ)
AMYY: YIM_11615(fdtB1) YIM_22860(fdtB2)
AORI: SD37_33620
SESP: BN6_58910(sam30)
KAL: KALB_6580(acuN1)
ACTI: UA75_05680
ACAD: UA74_05675
AHG: AHOG_05440(fdtB1)
ALO: CRK56086
SAQ: Sare_2337
MIL: ML5_4433
MCAB: HXZ27_04125(evdS12)
PLS: VT03_24275(fdtB)
AG: AAG13910(megDII) ABV49595(angB) BAC55216(staI)
 » show all
Reference
  Authors
Xu L, Huang H, Wei W, Zhong Y, Tang B, Yuan H, Zhu L, Huang W, Ge M, Yang S, Zheng H, Jiang W, Chen D, Zhao GP, Zhao W
  Title
Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis.
  Journal
BMC Genomics 15:363 (2014)
DOI:10.1186/1471-2164-15-363
  Sequence
[aoi:AORI_1500]
Reference
  Authors
Useglio M, Peiru S, Rodriguez E, Labadie GR, Carney JR, Gramajo H
  Title
TDP-L-megosamine biosynthesis pathway elucidation and megalomicin a production in Escherichia coli.
  Journal
Appl Environ Microbiol 76:3869-77 (2010)
DOI:10.1128/AEM.03083-09
  Sequence
Reference
  Authors
Rodriguez E, Peiru S, Carney JR, Gramajo H
  Title
In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea.
  Journal
Microbiology 152:667-73 (2006)
DOI:10.1099/mic.0.28680-0
  Sequence
Reference
  Authors
Schell U, Haydock SF, Kaja AL, Carletti I, Lill RE, Read E, Sheehan LS, Low L, Fernandez MJ, Grolle F, McArthur HA, Sheridan RM, Leadlay PF, Wilkinson B, Gaisser S
  Title
Engineered biosynthesis of hybrid macrolide polyketides containing D-angolosamine and D-mycaminose moieties.
  Journal
Org Biomol Chem 6:3315-27 (2008)
DOI:10.1039/b807914e
  Sequence
Reference
  Authors
Onaka H, Taniguchi S, Igarashi Y, Furumai T
  Title
Cloning of the staurosporine biosynthetic gene cluster from Streptomyces sp. TP-A0274 and its heterologous expression in Streptomyces lividans.
  Journal
J Antibiot (Tokyo) 55:1063-71 (2002)
DOI:10.7164/antibiotics.55.1063
  Sequence

KEGG   ENZYME: 2.6.1.106
Entry
EC 2.6.1.106                Enzyme                                 

Name
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase;
desV (gene name);
megDII (gene name);
eryCI (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose:2-oxoglutarate aminotransferase
Reaction(IUBMB)
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-3-dehydro-4,6-deoxy-alpha-D-glucose + L-glutamate [RN:R06426]
Reaction(KEGG)
R06426
Substrate
dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose [CPD:C11910];
2-oxoglutarate [CPD:C00026]
Product
dTDP-3-dehydro-4,6-deoxy-alpha-D-glucose [CPD:C11909];
L-glutamate [CPD:C00025]
Comment
A pyridoxal-phosphate protein. The enzyme is involved in the biosynthesis of dTDP-alpha-D-desosamine, a sugar found in several bacterial macrolide antibiotics including erythromycin, megalomicin A, mycinamicin II, and oleandomycin. The reaction occurs in the reverse direction.
History
EC 2.6.1.106 created 2014
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13310  dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase
K16436  dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase
Genes
DTX: ATSB10_36020
PPF: Pput_3928
PVD: CFBP1590__4426(fdtB)
OME: OLMES_5572
BOK: DM82_922
BOC: BG90_13
BUU: WS70_10345
VEI: Veis_0694
CSER: CCO03_02175
OTO: ADJ79_07890
GUR: Gura_4276
GBM: Gbem_3307
GEO: Geob_0430
GEM: GM21_0941
DMA: DMR_07600
DBR: Deba_0354
BRAD: BF49_6200
RPE: RPE_1521
MAGN: WV31_12980
SGR: SGR_631
SLE: sle_09260(sle_09260)
SRN: A4G23_00481(fdtB_1) A4G23_02284(fdtB_3)
SALJ: SMD11_1659
NCA: Noca_4220
NDA: Ndas_4709
NAL: B005_1433
STRR: EKD16_15580(fdtB)
TCU: Tcur_2777
SEN: SACE_0734(eryCI)
SACC: EYD13_16495(fdtB)
AOI: AORI_1500
AJA: AJAP_32020(dnrJ)
AMYY: YIM_11615(fdtB1) YIM_22860(fdtB2)
AORI: SD37_33620
SESP: BN6_58910(sam30)
KAL: KALB_6580(acuN1)
AHG: AHOG_05440(fdtB1) AHOG_15460(fdtB2)
ALO: CRK56086
SAQ: Sare_2337
MIL: ML5_4433
MCAB: HXZ27_04125(evdS12)
PLS: VT03_24275(fdtB)
 » show all
Reference
1  [PMID:17456741]
  Authors
Burgie ES, Thoden JB, Holden HM
  Title
Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine.
  Journal
Protein Sci 16:887-96 (2007)
DOI:10.1110/ps.062711007
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.106
IUBMB Enzyme Nomenclature: 2.6.1.106
ExPASy - ENZYME nomenclature database: 2.6.1.106
BRENDA, the Enzyme Database: 2.6.1.106

KEGG   REACTION: R06426
Entry
R06426                      Reaction                               

Name
dTDP-3-amino-3,4,6-trideoxy-D-glucose:2-oxoglutarate aminotransferase
Definition
dTDP-3-oxo-4,6-dideoxy-D-glucose + L-Glutamate <=> dTDP-3-amino-3,4,6-trideoxy-D-glucose + 2-Oxoglutarate
Equation
Reaction class
RC00006  C00025_C00026
RC01514  C11909_C11910
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00797  dTDP-D-desosamine biosynthesis
Orthology
K13310  dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose transaminase [EC:2.6.1.106]
K16436  dTDP-3-amino-2,3,6-trideoxy-4-keto-D-glucose/dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose/dTDP-2,6-dideoxy-D-kanosamine transaminase [EC:2.6.1.- 2.6.1.106]
Other DBs
RHEA: 39910

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