KEGG   ORTHOLOGY: K13312Help
Entry
K13312                      KO                                     

Name
tylJ, gerF
Definition
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00794  dTDP-6-deoxy-D-allose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13312  tylJ, gerF; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.3  Acting on carbohydrates and derivatives
    5.1.3.27  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
     K13312  tylJ, gerF; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
BRITE hierarchy
Other DBs
RN: R06437
Genes
STRM: M444_29900
AG: AAD12169(tylJ) ABB52524(gerF)
TaxonomyKoalaUniProt
Reference
PMID:10220165 (tylJ)
  Authors
Fouces R, Mellado E, Diez B, Barredo JL
  Title
The tylosin biosynthetic cluster from Streptomyces fradiae: genetic organization of the left region.
  Journal
Microbiology 145 ( Pt 4):855-68 (1999)
DOI:10.1099/13500872-145-4-855
  Sequence
Reference
PMID:17053005 (gerF)
  Authors
Thuy TT, Liou K, Oh TJ, Kim DH, Nam DH, Yoo JC, Sohng JK
  Title
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP.
  Journal
Glycobiology 17:119-26 (2007)
DOI:10.1093/glycob/cwl060
  Sequence

KEGG   ORTHOLOGY: K19855Help
Entry
K19855                      KO                                     

Name
novW
Definition
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00795  dTDP-beta-L-noviose biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K19855  novW; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Enzymes [BR:ko01000]
 5. Isomerases
  5.1  Racemases and epimerases
   5.1.3  Acting on carbohydrates and derivatives
    5.1.3.27  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
     K19855  novW; dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
BRITE hierarchy
Other DBs
RN: R06437
Genes
NGV: CDO52_02260
AG: AAF67516(novW) AAG29805(couW) AAN65245(cloW)
TaxonomyKoalaUniProt
Reference
  Authors
Steffensky M, Muhlenweg A, Wang ZX, Li SM, Heide L
  Title
Identification of the novobiocin biosynthetic gene cluster of Streptomyces spheroides NCIB 11891.
  Journal
Antimicrob Agents Chemother 44:1214-22 (2000)
DOI:10.1128/AAC.44.5.1214-1222.2000
  Sequence
Reference
  Authors
Tello M, Jakimowicz P, Errey JC, Freel Meyers CL, Walsh CT, Buttner MJ, Lawson DM, Field RA
  Title
Characterisation of Streptomyces spheroides NovW and revision of its functional assignment to a dTDP-6-deoxy-D-xylo-4-hexulose 3-epimerase.
  Journal
Chem Commun (Camb) 1079-81 (2006)
DOI:10.1039/b515763c

KEGG   ENZYME: 5.1.3.27Help
Entry
EC 5.1.3.27                 Enzyme                                 

Name
dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase;
dTDP-deoxyglucose 3-epimerase;
dTDP-4-keto-6-deoxy-D-glucose 3-epimerase;
dTDP-4-keto-6-deoxyglucose 3-epimerase;
gerF (gene name);
tylJ (gene name);
chmJ (gene name);
mydH (gene name)
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
Reaction(IUBMB)
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-gulose [RN:R06437]
Reaction(KEGG)
Substrate
dTDP-4-dehydro-6-deoxy-alpha-D-glucose [CPD:C11907]
Product
dTDP-4-dehydro-6-deoxy-alpha-D-gulose [CPD:C11926]
Comment
The enzyme is involved in the biosynthetic pathway of dTDP-6-deoxy-alpha-D-allose, which is converted to mycinose after attachment to the aglycones of several macrolide antibiotics, including tylosin, chalcomycin, dihydrochalcomycin, and mycinamicin II.
History
EC 5.1.3.27 created 2013
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K13312  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
K19855  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase
Genes
STRM: M444_29900
NGV: CDO52_02260
Taxonomy
Reference
1  [PMID:15049360]
  Authors
Sohng JK, Kim HJ, Nam DH, Lim DO, Han JM, Lee HJ, Yoo JC
  Title
Cloning, expression, and biological function of a dTDP-deoxyglucose epimerase (gerF) gene from Streptomyces sp. GERI-155.
  Journal
Biotechnol Lett 26:185-91 (2004)
  Sequence
Reference
2  [PMID:17053005]
  Authors
Thuy TT, Liou K, Oh TJ, Kim DH, Nam DH, Yoo JC, Sohng JK
  Title
Biosynthesis of dTDP-6-deoxy-beta-D-allose, biochemical characterization of dTDP-4-keto-6-deoxyglucose reductase (GerKI) from Streptomyces sp. KCTC 0041BP.
  Journal
Glycobiology 17:119-26 (2007)
DOI:10.1093/glycob/cwl060
  Sequence
Reference
3  [PMID:23116432]
  Authors
Kubiak RL, Phillips RK, Zmudka MW, Ahn MR, Maka EM, Pyeatt GL, Roggensack SJ, Holden HM
  Title
Structural and functional studies on a 3'-epimerase involved in the biosynthesis  of dTDP-6-deoxy-D-allose.
  Journal
Biochemistry 51:9375-83 (2012)
DOI:10.1021/bi3012737
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.27
IUBMB Enzyme Nomenclature: 5.1.3.27
ExPASy - ENZYME nomenclature database: 5.1.3.27
BRENDA, the Enzyme Database: 5.1.3.27

KEGG   REACTION: R06437Help
Entry
R06437                      Reaction                               

Name
dTDP-4-dehydro-6-deoxy-alpha-D-glucose 3-epimerase
Definition
dTDP-4-oxo-6-deoxy-D-glucose <=> dTDP-4-oxo-6-deoxy-D-allose
Equation
Reaction class
RC01519  C11907_C11926
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00794  dTDP-6-deoxy-D-allose biosynthesis
M00795  dTDP-beta-L-noviose biosynthesis
Orthology
K13312  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
K19855  dTDP-4-dehydro-6-deoxy-D-glucose 3-epimerase [EC:5.1.3.27]
Other DBs
RHEA: 36974

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