KEGG   ORTHOLOGY: K13328
Entry
K13328                      KO                                     

Name
spnQ
Definition
dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase [EC:4.2.1.164]
Pathway
ko00523  Polyketide sugar unit biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00802  dTDP-D-forosamine biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00523 Polyketide sugar unit biosynthesis
    K13328  spnQ; dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.164  dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
     K13328  spnQ; dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
Other DBs
RN: R08930
COG: COG0399
Genes
AGE: AA314_01884
NBR: O3I_015225
SBH: SBI_06871 SBI_08410(nanG3)
SALS: SLNWT_0672
SCI: B446_13485
SALU: DC74_6602
SALL: SAZ_34325
SVT: SVTN_18825
SLC: SL103_12605
SAMB: SAM23877_5613(srm24)
SLX: SLAV_40180(vioA)
SFK: KY5_2311c
NDA: Ndas_4697
NAL: B005_1455(ddhC)
ACTW: F7P10_30415(rfbH)
SACG: FDZ84_24190(rfbH)
AORI: SD37_34295
SAQ: Sare_1264
MICH: FJK98_15555(rfbH)
AG: AAG23278(spnQ)
 » show all
Reference
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Reference
  Authors
Waldron C, Matsushima P, Rosteck PR Jr, Broughton MC, Turner J, Madduri K, Crawford KP, Merlo DJ, Baltz RH
  Title
Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa.
  Journal
Chem Biol 8:487-99 (2001)
DOI:10.1016/S1074-5521(01)00029-1
  Sequence

KEGG   ENZYME: 4.2.1.164
Entry
EC 4.2.1.164                Enzyme                                 

Name
dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase;
SpnQ;
TDP-4-keto-2,6-dideoxy-D-glucose 3-dehydrase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose hydro-lyase (dTDP-2,3,6-trideoxy-alpha-D-hexopyranose-forming)
Reaction(IUBMB)
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + H2O + 2 oxidized ferredoxin [iron-sulfur] cluster [RN:R08930]
Reaction(KEGG)
R08930
Substrate
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose [CPD:C11922];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080]
Product
dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose [CPD:C18032];
H2O [CPD:C00001];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139]
Comment
A pyridoxal 5'-phosphate protein. The enzyme, isolated from the bacterium Saccharopolyspora spinosa, participates in the biosynthesis of forosamine. Requires ferredoxin/ferredoxin reductase or flavodoxin/flavodoxin reductase [1].
History
EC 4.2.1.164 created 2016
Pathway
ec00523  Polyketide sugar unit biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13328  dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase
Genes
AGE: AA314_01884
NBR: O3I_015225
SBH: SBI_06871 SBI_08410(nanG3)
SALS: SLNWT_0672
SCI: B446_13485
SALU: DC74_6602
SALL: SAZ_34325
SVT: SVTN_18825
SLC: SL103_12605
SAMB: SAM23877_5613(srm24)
SLX: SLAV_40180(vioA)
SFK: KY5_2311c
NDA: Ndas_4697
NAL: B005_1455(ddhC)
ACTW: F7P10_30415(rfbH)
SACG: FDZ84_24190(rfbH)
AORI: SD37_34295
SAQ: Sare_1264
MICH: FJK98_15555(rfbH)
 » show all
Reference
1  [PMID:17076492]
  Authors
Hong L, Zhao Z, Liu HW.
  Title
Characterization of SpnQ from the spinosyn biosynthetic pathway of Saccharopolyspora spinosa: mechanistic and evolutionary implications for C-3 deoxygenation in deoxysugar biosynthesis.
  Journal
J Am Chem Soc 128:14262-3 (2006)
DOI:10.1021/ja0649670
Reference
2  [PMID:18345667]
  Authors
Hong L, Zhao Z, Melancon CE 3rd, Zhang H, Liu HW.
  Title
In vitro characterization of the enzymes involved in TDP-D-forosamine biosynthesis in the spinosyn pathway of Saccharopolyspora spinosa.
  Journal
J Am Chem Soc 130:4954-67 (2008)
DOI:10.1021/ja0771383
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.164
IUBMB Enzyme Nomenclature: 4.2.1.164
ExPASy - ENZYME nomenclature database: 4.2.1.164
BRENDA, the Enzyme Database: 4.2.1.164

KEGG   REACTION: R08930
Entry
R08930                      Reaction                               

Name
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose hydro-lyase (dTDP-2,3,6-trideoxy-alpha-D-hexopyranose-forming)
Definition
dTDP-4-oxo-2,6-dideoxy-D-glucose + Reduced ferredoxin + H+ <=> dTDP-4-oxo-2,3,6-trideoxy-D-glucose + H2O + Oxidized ferredoxin
Equation
Reaction class
RC00704  C11922_C18032
Enzyme
Pathway
rn00523  Polyketide sugar unit biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00802  dTDP-D-forosamine biosynthesis
Orthology
K13328  dTDP-4-dehydro-2,6-dideoxy-D-glucose 3-dehydratase [EC:4.2.1.164]

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