KEGG   ORTHOLOGY: K13547Help
Entry
K13547                      KO                                     

Name
btrR, neoB, kanB
Definition
L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase [EC:2.6.1.100 2.6.1.101]
Pathway
ko00524  Neomycin, kanamycin and gentamicin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K13547  btrR, neoB, kanB; L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01007 Amino acid related enzymes
    K13547  btrR, neoB, kanB; L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.6  Transferring nitrogenous groups
   2.6.1  Transaminases
    2.6.1.100  L-glutamine:2-deoxy-scyllo-inosose aminotransferase
     K13547  btrR, neoB, kanB; L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
    2.6.1.101  L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
     K13547  btrR, neoB, kanB; L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Amino acid related enzymes [BR:ko01007]
 Aminotransferase (transaminase)
  Others
   K13547  btrR, neoB, kanB; L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
BRITE hierarchy
Other DBs
RN: R08890 R08891
Genes
SRN: A4G23_05129(neoB)
AG: BAD95819(neoB) BAE07061(btrR) CAE46938(kanB)
TaxonomyKoalaUniProt
Reference
PMID:16156513 (btrR)
  Authors
Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K
  Title
Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
  Journal
J Antibiot (Tokyo) 58:373-9 (2005)
DOI:10.1038/ja.2005.47
  Sequence
Reference
PMID:16506694 (neoB)
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
PMID:15313224 (kanB)
  Authors
Kharel MK, Subba B, Basnet DB, Woo JS, Lee HC, Liou K, Sohng JK
  Title
A gene cluster for biosynthesis of kanamycin from Streptomyces kanamyceticus: comparison with gentamicin biosynthetic gene cluster.
  Journal
Arch Biochem Biophys 429:204-14 (2004)
DOI:10.1016/j.abb.2004.06.009
  Sequence
Reference
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.
  Journal
Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5
Reference
  Authors
Yokoyama K, Kudo F, Kuwahara M, Inomata K, Tamegai H, Eguchi T, Kakinuma K
  Title
Stereochemical recognition of doubly functional aminotransferase in 2-deoxystreptamine biosynthesis.
  Journal
J Am Chem Soc 127:5869-74 (2005)
DOI:10.1021/ja0445948

KEGG   ENZYME: 2.6.1.101Help
Entry
EC 2.6.1.101                Enzyme                                 

Name
L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
L-glutamine:5-amino-2,3,4-trihydroxycyclohexanone aminotransferase
Reaction(IUBMB)
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxystreptamine [RN:R08891]
Reaction(KEGG)
Substrate
L-glutamine [CPD:C00064];
3-amino-2,3-dideoxy-scyllo-inosose [CPD:C17581]
Product
2-oxoglutaramate [CPD:C00940];
2-deoxystreptamine [CPD:C02627]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including kanamycin, butirosin, neomycin and ribostamycin. Also catalyses EC 2.6.1.100, L-glutamine:2-deoxy-scyllo-inosose aminotransferase.
History
EC 2.6.1.101 created 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13547  L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Genes
SRN: A4G23_05129(neoB)
Taxonomy
Reference
1  [PMID:15827636]
  Authors
Huang F, Haydock SF, Mironenko T, Spiteller D, Li Y, Spencer JB
  Title
The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine.
  Journal
Org Biomol Chem 3:1410-8 (2005)
DOI:10.1039/b501199j
Reference
2  [PMID:16506694]
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K.
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.101
IUBMB Enzyme Nomenclature: 2.6.1.101
ExPASy - ENZYME nomenclature database: 2.6.1.101
BRENDA, the Enzyme Database: 2.6.1.101

KEGG   REACTION: R08891Help
Entry
R08891                      Reaction                               

Name
L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase
Definition
3-Amino-2,3-dideoxy-scyllo-inosose + L-Glutamine <=> 2-Deoxystreptamine + 2-Oxoglutaramate
Equation
Reaction class
RC00006  C00064_C00940
RC01514  C02627_C17581
Enzyme
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K13547  L-glutamine:2-deoxy-scyllo-inosose/3-amino-2,3-dideoxy-scyllo-inosose aminotransferase [EC:2.6.1.100 2.6.1.101]
Other DBs
RHEA: 34154

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