KEGG   ORTHOLOGY: K13554Help
Entry
K13554                      KO                                     

Name
btrL, neoM
Definition
neamine phosphoribosyltransferase [EC:2.4.2.49]
Pathway
ko00524  Neomycin, kanamycin and gentamicin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K13554  btrL, neoM; neamine phosphoribosyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.2  Pentosyltransferases
    2.4.2.49  neamine phosphoribosyltransferase
     K13554  btrL, neoM; neamine phosphoribosyltransferase
BRITE hierarchy
Other DBs
RN: R08898
Genes
SRN: A4G23_05118(btrL)
AG: BAD95830(neoM) BAE07064(btrL)
TaxonomyKoalaUniProt
Reference
PMID:16156513 (btrL)
  Authors
Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K
  Title
Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
  Journal
J Antibiot (Tokyo) 58:373-9 (2005)
DOI:10.1038/ja.2005.47
  Sequence
Reference
PMID:16506694 (neoM)
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.
  Journal
Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5
Reference
  Authors
Kudo F, Fujii T, Kinoshita S, Eguchi T
  Title
Unique O-ribosylation in the biosynthesis of butirosin.
  Journal
Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040

KEGG   ENZYME: 2.4.2.49Help
Entry
EC 2.4.2.49                 Enzyme                                 

Name
neamine phosphoribosyltransferase;
btrL (gene name);
neoM (gene name)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
neamine:5-phospho-alpha-D-ribose 1-diphosphate phosphoribosyltransferase
Reaction(IUBMB)
neamine + 5-phospho-alpha-D-ribose 1-diphosphate = 5''-phosphoribostamycin + diphosphate [RN:R08898]
Reaction(KEGG)
Substrate
neamine [CPD:C01441];
5-phospho-alpha-D-ribose 1-diphosphate [CPD:C00119]
Product
5''-phosphoribostamycin [CPD:C18004];
diphosphate [CPD:C00013]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. The enzyme requires a divalent metal ion, optimally Mg2+, Ni2+ or Co2+.
History
EC 2.4.2.49 created 2013
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13554  neamine phosphoribosyltransferase
Genes
SRN: A4G23_05118(btrL)
Taxonomy
Reference
1  [PMID:17482823]
  Authors
Kudo F, Fujii T, Kinoshita S, Eguchi T.
  Title
Unique O-ribosylation in the biosynthesis of butirosin.
  Journal
Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.49
IUBMB Enzyme Nomenclature: 2.4.2.49
ExPASy - ENZYME nomenclature database: 2.4.2.49
BRENDA, the Enzyme Database: 2.4.2.49

KEGG   REACTION: R08898Help
Entry
R08898                      Reaction                               

Name
5-phospho-alpha-D-ribose 1-diphosphate:neamine phosphoribosyltransferase;
5''-phosphoribostamycin:diphosphate phospho-alpha-D-ribosyltransferase
Definition
5-Phospho-alpha-D-ribose 1-diphosphate + Neamine <=> Diphosphate + 5''-Phosphoribostamycin
Equation
Reaction class
RC00049  C01441_C18004
Enzyme
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K13554  neamine phosphoribosyltransferase [EC:2.4.2.49]
Other DBs
RHEA: 34046

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