KEGG   ORTHOLOGY: K13555Help
Entry
K13555                      KO                                     

Name
btrP, neoI
Definition
5''-phosphoribostamycin phosphatase [EC:3.1.3.88]
Pathway
ko00524  Neomycin, kanamycin and gentamicin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K13555  btrP, neoI; 5''-phosphoribostamycin phosphatase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.1  Acting on ester bonds
   3.1.3  Phosphoric-monoester hydrolases
    3.1.3.88  5''-phosphoribostamycin phosphatase
     K13555  btrP, neoI; 5''-phosphoribostamycin phosphatase
BRITE hierarchy
Other DBs
RN: R08899
Genes
SRN: A4G23_05122(btrP)
AG: BAD95826(neoI) BAE07077(btrP)
TaxonomyKoalaUniProt
Reference
PMID:16156513 (btrP)
  Authors
Kudo F, Numakura M, Tamegai H, Yamamoto H, Eguchi T, Kakinuma K
  Title
Extended sequence and functional analysis of the butirosin biosynthetic gene cluster in Bacillus circulans SANK 72073.
  Journal
J Antibiot (Tokyo) 58:373-9 (2005)
DOI:10.1038/ja.2005.47
  Sequence
Reference
PMID:16506694 (neoI)
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.
  Journal
Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5
Reference
  Authors
Kudo F, Fujii T, Kinoshita S, Eguchi T
  Title
Unique O-ribosylation in the biosynthesis of butirosin.
  Journal
Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040

KEGG   ENZYME: 3.1.3.88Help
Entry
EC 3.1.3.88                 Enzyme                                 

Name
5''-phosphoribostamycin phosphatase;
btrP (gene name);
neoI (gene name)
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
BRITE hierarchy
Sysname
5''-phosphoribostamycin phosphohydrolase
Reaction(IUBMB)
5''-phosphoribostamycin + H2O = ribostamycin + phosphate [RN:R08899]
Reaction(KEGG)
Substrate
5''-phosphoribostamycin [CPD:C18004];
H2O [CPD:C00001]
Product
ribostamycin [CPD:C01759];
phosphate [CPD:C00009]
Comment
Involved in the biosynthetic pathways of several clinically important aminocyclitol antibiotics, including ribostamycin, neomycin and butirosin. No metal is required for activity.
History
EC 3.1.3.88 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13555  5''-phosphoribostamycin phosphatase
Genes
SRN: A4G23_05122(btrP)
Taxonomy
Reference
1  [PMID:17482823]
  Authors
Kudo F, Fujii T, Kinoshita S, Eguchi T.
  Title
Unique O-ribosylation in the biosynthesis of butirosin.
  Journal
Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 3.1.3.88
IUBMB Enzyme Nomenclature: 3.1.3.88
ExPASy - ENZYME nomenclature database: 3.1.3.88
BRENDA, the Enzyme Database: 3.1.3.88

KEGG   REACTION: R08899Help
Entry
R08899                      Reaction                               

Name
5''-phosphoribostamycin phosphohydrolase
Definition
5''-Phosphoribostamycin + H2O <=> Ribostamycin + Orthophosphate
Equation
Reaction class
RC00017  C01759_C18004
Enzyme
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K13555  5''-phosphoribostamycin phosphatase [EC:3.1.3.88]
Reference
1  [PMID:17482823]
  Authors
Kudo F, Fujii T, Kinoshita S, Eguchi T.
  Title
Unique O-ribosylation in the biosynthesis of butirosin.
  Journal
Bioorg Med Chem 15:4360-8 (2007)
DOI:10.1016/j.bmc.2007.04.040
Other DBs
RHEA: 33974

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