KEGG   ORTHOLOGY: K13556Help
Entry
K13556                      KO                                     

Name
neoK
Definition
UDP-N-acetyl-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase [EC:2.4.1.285]
Pathway
ko00524  Neomycin, kanamycin and gentamicin biosynthesis
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00524 Neomycin, kanamycin and gentamicin biosynthesis
    K13556  neoK; UDP-N-acetyl-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.285  UDP-GlcNAc:ribostamycin N-acetylglucosaminyltransferase
     K13556  neoK; UDP-N-acetyl-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase
BRITE hierarchy
Other DBs
RN: R08900
Genes
SRN: A4G23_05120(neoK)
AG: BAD95828(neoK)
TaxonomyKoalaUniProt
Reference
  Authors
Kudo F, Yamamoto Y, Yokoyama K, Eguchi T, Kakinuma K
  Title
Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773.
  Journal
J Antibiot (Tokyo) 58:766-74 (2005)
DOI:10.1038/ja.2005.104
  Sequence
Reference
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic enzymes for the aminoglycosides butirosin and neomycin.
  Journal
Methods Enzymol 459:493-519 (2009)
DOI:10.1016/S0076-6879(09)04620-5
Reference
  Authors
Kudo F, Eguchi T
  Title
Biosynthetic genes for aminoglycoside antibiotics.
  Journal
J Antibiot (Tokyo) 62:471-81 (2009)
DOI:10.1038/ja.2009.76

KEGG   ENZYME: 2.4.1.285Help
Entry
EC 2.4.1.285                Enzyme                                 

Name
UDP-GlcNAc:ribostamycin N-acetylglucosaminyltransferase;
neoK (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:ribostamycin N-acetylglucosaminyltransferase
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + ribostamycin = UDP + 2'''-acetyl-6'''-hydroxyneomycin C [RN:R08900]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
ribostamycin [CPD:C01759]
Product
UDP [CPD:C00015];
2'''-acetyl-6'''-hydroxyneomycin C
Comment
Involved in biosynthesis of the aminoglycoside antibiotic neomycin. Requires a divalent metal ion, optimally Mg2+, Mn2+ or Co2+.
History
EC 2.4.1.285 created 2012
Pathway
ec00524  Neomycin, kanamycin and gentamicin biosynthesis
ec01130  Biosynthesis of antibiotics
Orthology
K13556  UDP-N-acetyl-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase
Genes
SRN: A4G23_05120(neoK)
Taxonomy
Reference
1  [PMID:18311744]
  Authors
Yokoyama K, Yamamoto Y, Kudo F, Eguchi T.
  Title
Involvement of two distinct N-acetylglucosaminyltransferases and a dual-function  deacetylase in neomycin biosynthesis.
  Journal
Chembiochem 9:865-9 (2008)
DOI:10.1002/cbic.200700717
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.285
IUBMB Enzyme Nomenclature: 2.4.1.285
ExPASy - ENZYME nomenclature database: 2.4.1.285
BRENDA, the Enzyme Database: 2.4.1.285

KEGG   REACTION: R08900Help
Entry
R08900                      Reaction                               

Name
UDP-N-acetyl-alpha-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase
Definition
UDP-N-acetyl-alpha-D-glucosamine + Ribostamycin <=> UDP + 2'''-N-Acetyl-6'''-deamino-6'''-hydroxyneomycin C
Equation
Reaction class
RC00005  C00015_C00043
RC00049  C01759_C17587
Enzyme
Pathway
rn00524  Neomycin, kanamycin and gentamicin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K13556  UDP-N-acetyl-D-glucosamine:ribostamycin N-acetyl-D-glucosaminyltransferase [EC:2.4.1.285]
Other DBs
RHEA: 33954

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