KEGG   ORTHOLOGY: K14028
Entry
K14028                      KO                                     

Name
mdh1, mxaF
Definition
methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
Pathway
ko00010  Glycolysis / Gluconeogenesis
ko00620  Pyruvate metabolism
ko00625  Chloroalkane and chloroalkene degradation
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
   00620 Pyruvate metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  09102 Energy metabolism
   00680 Methane metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  09111 Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
Other DBs
RN: R01146 R09127 R09128 R10713
GO: 0052930
Genes
DKO: I596_1067
MCA: MCA0779(mxaF)
METU: GNH96_04380
MMT: Metme_4317
MDN: JT25_020965
MDH: AYM39_15615
MKO: MKLM6_3210
METL: U737_12050
MAH: MEALZ_3448(mxaF)
MBUR: EQU24_18145
MMAI: sS8_1436
MEJ: Q7A_537
MEC: Q7C_2125
VEI: Veis_1829
MFA: Mfla_2044
MEP: MPQ_0771
RPC: RPC_1907
RPE: RPE_3139
SNO: Snov_4188
MEA: Mex_1p4538(mxaF)
MDI: METDI5145(mxaF)
MEX: Mext_4150
MCH: Mchl_4518
MPO: Mpop_4632
MNO: Mnod_8040
MOR: MOC_4862(mxaF)
META: Y590_20685
MAQU: Maq22A_1p33165(mxaF)
MSL: Msil_0471
MTUN: MTUNDRAET4_1612(mxaF)
HDN: Hden_1323
HMC: HYPMC_0485(mxaF)
FIL: BN1229_v1_2515(mxaF)
FIY: BN1229_v1_3401(mxaF)
MSC: BN69_2570(mxaF)
PLEO: OHA_1_04073(mxaF)
MCG: GL4_0421
PDE: Pden_2993
ALI: AZOLI_p10948(moxF1)
MOX: DAMO_0112(mxaF)
 » show all
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence

KEGG   ORTHOLOGY: K16254
Entry
K16254                      KO                                     

Name
mxaJ
Definition
mxaJ protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16254  mxaJ; mxaJ protein
Genes
LSOL: GOY17_09790
DTX: ATSB10_32670
DKO: I596_1066
HMI: soil367_04250
MCA: MCA0780
METU: GNH96_04385(moxJ)
MMT: Metme_4318
MDN: JT25_020970
MDH: AYM39_15610
MKO: MKLM6_3209
METL: U737_12045(moxJ)
MAH: MEALZ_3447(mxaJ)
MBUR: EQU24_18140(moxJ)
MMOB: F6R98_08160(moxJ)
MEJ: Q7A_536(moxJ)
MEC: Q7C_2124
AMIM: MIM_c14440
VEI: Veis_1828
MMB: Mmol_2047
MEP: MPQ_0772(hisJ) MPQ_2305
MBAT: BN1208_1207(xoxJ)
BRAD: BF49_5267
BRK: CWS35_16225(moxJ)
RPC: RPC_1908
RPE: RPE_3138
SNO: Snov_4189
ANC: GBB76_11855(moxJ)
APRA: G3A50_20135(moxJ)
MEA: Mex_1p4537(mxaJ)
MDI: METDI5143(mxaJ)
MCH: Mchl_4517
MPO: Mpop_4631
MOR: MOC_4861(moxJ)
META: Y590_20680
MAQU: Maq22A_1p33170(mxaJ) Maq22A_c05225(mxaJ)
METD: C0214_22755(moxJ)
METS: DK389_08305(moxJ)
METI: DK427_25665(moxJ)
MMES: MMSR116_00885(moxJ)
HDN: Hden_1322
FIL: BN1229_v1_2516(mxaJ)
FIY: BN1229_v1_3400(mxaJ)
MROS: EHO51_16430(moxJ)
MPAR: F7D14_04030(moxJ)
AALA: IGS74_17250(moxJ)
MCG: GL4_0422
PDE: Pden_2994
PYE: A6J80_13915(moxJ)
ALI: AZOLI_p10949(moxJ)
NAO: Y958_26260(moxJ)
LPIL: LIP_1127
MKC: kam1_1016
GBA: J421_1954
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K16255
Entry
K16255                      KO                                     

Name
mxaG
Definition
cytochrome c-L
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16255  mxaG; cytochrome c-L
Other DBs
COG: COG2010
Genes
DKO: I596_1065
MCA: MCA0781
METU: GNH96_04390(moxG)
MMT: Metme_4319
MDN: JT25_020975
MDH: AYM39_15605
MKO: MKLM6_3208
METL: U737_12040(moxG)
MAH: MEALZ_3446(mxaG)
MBUR: EQU24_18135(moxG)
MPSY: CEK71_12445(moxG)
MMAI: sS8_1434
MMOB: F6R98_08165(moxG)
MEJ: Q7A_535(moxG)
MEC: Q7C_2123
GAI: IMCC3135_09480(moxG)
VEI: Veis_1827
MFA: Mfla_2042
MMB: Mmol_2045
MBAT: BN1208_1206(xoxG)
BRK: CWS35_16230(moxG)
RPC: RPC_1909
RPE: RPE_3137
NHA: Nham_0990
SNO: Snov_4190
ANC: GBB76_11860(moxG)
APRA: G3A50_20140(moxG)
MEA: Mex_1p4536(mxaG)
MDI: METDI5142(mxaG)
MEX: Mext_4148
MCH: Mchl_4516
MPO: Mpop_4630
MNO: Mnod_8038
MOR: MOC_4860(moxG)
META: Y590_20675
MAQU: Maq22A_1p33175(mxaG)
METD: C0214_22750(moxG)
METS: DK389_08300(moxG)
METI: DK427_25660(moxG)
MMES: MMSR116_00890(moxG)
MSL: Msil_0473
MTUN: MTUNDRAET4_1610(mxaG)
MLG: CWB41_12375(moxG)
HMC: HYPMC_0487(mxaG)
FIL: BN1229_v1_2517(mxaG)
FIY: BN1229_v1_3399(mxaG)
MSC: BN69_2572
MROS: EHO51_16435(moxG)
MHEY: H2LOC_010110(moxG)
MPAR: F7D14_04025(moxG)
MTW: CQW49_14435(moxG)
PLEO: OHA_1_04075(mxaG)
AALA: IGS74_17255(moxG)
MCG: GL4_0423
PDE: Pden_2995
PYE: A6J80_13910(moxG)
ALI: AZOLI_p10950(moxG)
NAO: Y958_26255(moxG)
MOX: DAMO_0114(mxaG) DAMO_0138(mxaG)
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K14029
Entry
K14029                      KO                                     

Name
mdh2, mxaI
Definition
methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
Pathway
ko00010  Glycolysis / Gluconeogenesis
ko00620  Pyruvate metabolism
ko00625  Chloroalkane and chloroalkene degradation
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
   00620 Pyruvate metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  09102 Energy metabolism
   00680 Methane metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  09111 Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
Other DBs
RN: R01146 R09127 R09128 R10713
GO: 0052930
Genes
DKO: I596_1064
MCA: MCA0782(mxaI)
METU: GNH96_04395
MMT: Metme_4320
MDN: JT25_020980
MDH: AYM39_15600
MKO: MKLM6_3207
METL: U737_12035
MAH: MEALZ_3445(mxaI)
MBUR: EQU24_18130
MMAI: sS8_1433
MEJ: Q7A_534
MEC: Q7C_2122
VEI: Veis_1826
MFA: Mfla_2041
MEP: MPQ_0774
RPC: RPC_1910
RPE: RPE_3136
SNO: Snov_4191
MEA: Mex_1p4535(mxaI)
MDI: METDI5141(mxaI)
MEX: Mext_4147
MCH: Mchl_4515
MPO: Mpop_4629
MNO: Mnod_8037
MOR: MOC_4859
META: Y590_20670
MAQU: Maq22A_1p33180(mxaI)
MSL: Msil_0474
MTUN: MTUNDRAET4_1609(mxaI)
HDN: Hden_1320
HMC: HYPMC_0488(mxaI)
FIL: BN1229_v1_2518(mxaI)
FIY: BN1229_v1_3398(mxaI)
MSC: BN69_2573
PLEO: OHA_1_04076(mxaI)
MCG: GL4_0424
PDE: Pden_2996
ALI: AZOLI_p10951(moxI)
MOX: DAMO_0115(mxaI)
 » show all
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence

KEGG   ORTHOLOGY: K16256
Entry
K16256                      KO                                     

Name
mxaA
Definition
mxaA protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16256  mxaA; mxaA protein
Genes
DKO: I596_1061
MCA: MCA0785
METU: GNH96_07705
MMT: Metme_4325
MDN: JT25_021000
MDH: AYM39_15580
MKO: MKLM6_3203
METL: U737_12010
MAH: MEALZ_3441
MBUR: EQU24_18110 EQU24_18330
BXB: DR64_4748
BPH: Bphy_3803
MPT: Mpe_A3276
RGE: RGE_23320
MFA: Mfla_0689
MMB: Mmol_1105
MEH: M301_2231
MEP: MPQ_2632
MBAT: BN1208_0636(mxaA)
RPC: RPC_1913
RPE: RPE_3133
SNO: Snov_4194
MEA: Mex_1p4532(mxaA)
MDI: METDI5138(mxaA)
MEX: Mext_4144
MCH: Mchl_4512
MPO: Mpop_4626
MNO: Mnod_8034
MOR: MOC_4856(mxaA)
META: Y590_20655
MAQU: Maq22A_1p33195(mxaA)
MSL: Msil_0477
MTUN: MTUNDRAET4_1605(mxaA)
HDN: Hden_1317
FIL: BN1229_v1_2521(mxaA)
FIY: BN1229_v1_3395(mxaA)
MSC: BN69_2576
PLEO: OHA_1_04079(mxaA)
MCG: GL4_0427
PDE: Pden_2999
MOX: DAMO_0118
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K16257
Entry
K16257                      KO                                     

Name
mxaC
Definition
mxaC protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16257  mxaC; mxaC protein
Genes
DKO: I596_1060
MCA: MCA0786 MCA1528
METU: GNH96_04415 GNH96_07700
MMT: Metme_4326
MDN: JT25_021005
MDH: AYM39_15575
MKO: MKLM6_3202
METL: U737_12005
MAH: MEALZ_3440(mxaC) MEALZ_3488(mxaC)
MBUR: EQU24_18105 EQU24_18335
BXE: Bxe_B2418
BXB: DR64_4747
BPH: Bphy_3804
RGE: RGE_23310
MFA: Mfla_1898
MMB: Mmol_1544
MEH: M301_1090
MEP: MPQ_0904
MBAT: BN1208_0635(mxaC)
RPC: RPC_1914
RPE: RPE_3132
SNO: Snov_4195
MEA: Mex_1p4531(mxaC)
MDI: METDI5137(mxaC)
MEX: Mext_4143
MCH: Mchl_4511
MPO: Mpop_4625
MNO: Mnod_8033
MOR: MOC_4855
META: Y590_20650
MAQU: Maq22A_1p33205(mxaC)
MSL: Msil_0478
MTUN: MTUNDRAET4_1604(mxaC)
HDN: Hden_1316
HMC: HYPMC_0492(mxaC)
MSC: BN69_2577
PLEO: OHA_1_04080(mxaC)
MCG: GL4_0428
PDE: Pden_3000
MOX: DAMO_0119
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K16258
Entry
K16258                      KO                                     

Name
mxaK
Definition
mxaK protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16258  mxaK; mxaK protein
Genes
DKO: I596_1059
MCA: MCA0787 MCA1529
METU: GNH96_04420 GNH96_07695
MMT: Metme_4327
MDN: JT25_021010
MDH: AYM39_15570
MKO: MKLM6_3201
METL: U737_12000
MAH: MEALZ_0661 MEALZ_3439(mxaK)
MBUR: EQU24_03260 EQU24_18100
BYI: BYI23_C003020(mxaL)
BUE: BRPE67_CCDS09200(mxaL)
BXE: Bxe_B2417
BXB: DR64_4746
BPH: Bphy_3805
RGE: RGE_23300(mxaK)
MMB: Mmol_1103
MEH: M301_2229
RPC: RPC_1915
RPE: RPE_3131
SNO: Snov_4196
MEA: Mex_1p4530(mxaK)
MDI: METDI5136(mxaK)
MEX: Mext_4142
MCH: Mchl_4510
MPO: Mpop_4624
MNO: Mnod_8032
MOR: MOC_4854(mxaK)
META: Y590_20645
MSL: Msil_0479
MTUN: MTUNDRAET4_1603(mxaK)
HDN: Hden_1315
MSC: BN69_2578
PLEO: OHA_1_04081(mxaK)
MCG: GL4_0429
PDE: Pden_3001
MOX: DAMO_0120
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K16259
Entry
K16259                      KO                                     

Name
mxaL
Definition
mxaL protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16259  mxaL; mxaL protein
Genes
DKO: I596_1058
MCA: MCA0788 MCA1530
METU: GNH96_04425 GNH96_07690
MMT: Metme_4328
MDN: JT25_021015
MDH: AYM39_15565
MKO: MKLM6_3200
METL: U737_11995
MAH: MEALZ_0662 MEALZ_3438(mxaL)
MBUR: EQU24_03265 EQU24_18095
BYI: BYI23_C003010(mxaL)
BUE: BRPE67_CCDS09210(mxaL)
BXE: Bxe_B2416
BXB: DR64_4745
BPH: Bphy_3806
RGE: RGE_23290(mxaL)
MBAT: BN1208_0633(mxaL)
RPC: RPC_1916
RPE: RPE_3130
SNO: Snov_4197
MEA: Mex_1p4529(mxaL)
MDI: METDI5135(mxaL)
MEX: Mext_4141
MCH: Mchl_4509
MPO: Mpop_4623
MNO: Mnod_8031
MOR: MOC_4853
META: Y590_20640
MAQU: Maq22A_1p33210(mxaL)
MSL: Msil_0480
MTUN: MTUNDRAET4_1602(mxaL)
HDN: Hden_1314
HMC: HYPMC_0494(mxaL)
MSC: BN69_2579
PLEO: OHA_1_04082(mxaL)
MCG: GL4_0430
PDE: Pden_3002
MOX: DAMO_0121
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ORTHOLOGY: K16260
Entry
K16260                      KO                                     

Name
mxaD
Definition
mxaD protein
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16260  mxaD; mxaD protein
Genes
DKO: I596_1057
MCA: MCA0789
METU: GNH96_04430
MMT: Metme_2323 Metme_4314
MDN: JT25_020955
MDH: AYM39_05840
MKO: MKLM6_1186
METL: U737_12065
MAH: MEALZ_3453(mxaD)
MBUR: EQU24_18160
MMAI: sS8_1426
VEI: Veis_1819
MFA: Mfla_2034
MEH: M301_0495
RPC: RPC_1917
RPE: RPE_3129
SNO: Snov_4198
MEA: Mex_1p4528(mxaD)
MDI: METDI5134(mxaD)
MEX: Mext_4140
MCH: Mchl_4508
MPO: Mpop_4622
MNO: Mnod_8030
MOR: MOC_4852(mxaD)
META: Y590_20635
MAQU: Maq22A_1p33215(mxaD)
MSL: Msil_0481
MTUN: MTUNDRAET4_1601(mxaD)
HDN: Hden_1313
PLEO: OHA_1_04083(mxaD)
MCG: GL4_0431
PDE: Pden_3003
 » show all
Reference
PMID:8997703
  Authors
Amaratunga K, Goodwin PM, O'Connor CD, Anthony C
  Title
The methanol oxidation genes mxaFJGIR (S) ACKLD in Methylobacterium extorquens.
  Journal
FEMS Microbiol Lett 146:31-8 (1997)
DOI:10.1111/j.1574-6968.1997.tb10167.x
Reference
  Authors
Luesken FA, Wu ML, Op den Camp HJ, Keltjens JT, Stunnenberg H, Francoijs KJ, Strous M, Jetten MS
  Title
Effect of oxygen on the anaerobic methanotroph 'Candidatus Methylomirabilis oxyfera': kinetic and transcriptional analysis.
  Journal
Environ Microbiol 14:1024-34 (2012)
DOI:10.1111/j.1462-2920.2011.02682.x
Reference
  Authors
Kalyuzhnaya MG, Hristova KR, Lidstrom ME, Chistoserdova L
  Title
Characterization of a novel methanol dehydrogenase in representatives of Burkholderiales: implications for environmental detection of methylotrophy and evidence for convergent evolution.
  Journal
J Bacteriol 190:3817-23 (2008)
DOI:10.1128/JB.00180-08

KEGG   ENZYME: 1.1.2.7
Entry
EC 1.1.2.7                  Enzyme                                 

Name
methanol dehydrogenase (cytochrome c);
methanol dehydrogenase;
MDH (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor
Sysname
methanol:cytochrome c oxidoreductase
Reaction(IUBMB)
a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ [RN:R10713]
Reaction(KEGG)
Substrate
primary alcohol [CPD:C00226];
ferricytochrome cL [CPD:C18233]
Product
aldehyde [CPD:C00071];
ferrocytochrome cL [CPD:C18234];
H+ [CPD:C00080]
Comment
A periplasmic quinoprotein alcohol dehydrogenase that only occurs in methylotrophic bacteria. It uses the novel specific cytochrome cL as acceptor. Acts on a wide range of primary alcohols, including ethanol, duodecanol, chloroethanol, cinnamyl alcohol, and also formaldehyde. Activity is stimulated by ammonia or methylamine. It is usually assayed with phenazine methosulfate. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ. It differs from EC 1.1.2.8, alcohol dehydrogenase (cytochrome c), in having a high affinity for methanol and in having a second essential small subunit (no known function).
History
EC 1.1.2.7 created 1972 as EC 1.1.99.8, modified 1982, part transferred 2010 to EC 1.1.2.7
Pathway
ec00010  Glycolysis / Gluconeogenesis
ec00620  Pyruvate metabolism
ec00625  Chloroalkane and chloroalkene degradation
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
ec01120  Microbial metabolism in diverse environments
Orthology
K14028  methanol dehydrogenase (cytochrome c) subunit 1
K14029  methanol dehydrogenase (cytochrome c) subunit 2
Genes
DKO: I596_1064 I596_1067
MCA: MCA0779(mxaF) MCA0782(mxaI)
METU: GNH96_04380 GNH96_04395
MMT: Metme_4317 Metme_4320
MDN: JT25_020965 JT25_020980
MDH: AYM39_15600 AYM39_15615
MKO: MKLM6_3207 MKLM6_3210
METL: U737_12035 U737_12050
MAH: MEALZ_3445(mxaI) MEALZ_3448(mxaF)
MBUR: EQU24_18130 EQU24_18145
MEA: Mex_1p4535(mxaI) Mex_1p4538(mxaF)
MDI: METDI5141(mxaI) METDI5145(mxaF)
MOR: MOC_4859 MOC_4862(mxaF)
MAQU: Maq22A_1p33165(mxaF) Maq22A_1p33180(mxaI)
MTUN: MTUNDRAET4_1609(mxaI) MTUNDRAET4_1612(mxaF)
HMC: HYPMC_0485(mxaF) HYPMC_0488(mxaI)
FIL: BN1229_v1_2515(mxaF) BN1229_v1_2518(mxaI)
FIY: BN1229_v1_3398(mxaI) BN1229_v1_3401(mxaF)
MSC: BN69_2570(mxaF) BN69_2573
PLEO: OHA_1_04073(mxaF) OHA_1_04076(mxaI)
ALI: AZOLI_p10948(moxF1) AZOLI_p10951(moxI)
MOX: DAMO_0112(mxaF) DAMO_0115(mxaI)
 » show all
Reference
1  [PMID:4378696]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27.
  Journal
Biochem J 92:614-21 (1964)
DOI:10.1042/bj0920614
Reference
2  [PMID:6049934]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.
  Journal
Biochem J 104:960-9 (1967)
DOI:10.1042/bj1040960
Reference
3  [PMID:6250827]
  Authors
Duine JA, Frank J, Verwiel PE.
  Title
Structure and activity of the prosthetic group of methanol dehydrogenase.
  Journal
Eur J Biochem 108:187-92 (1980)
DOI:10.1111/j.1432-1033.1980.tb04711.x
Reference
4  [PMID:471057]
  Authors
Salisbury SA, Forrest HS, Cruse WB, Kennard O.
  Title
A novel coenzyme from bacterial primary alcohol dehydrogenases.
  Journal
Nature 280:843-4 (1979)
DOI:10.1038/280843a0
Reference
5  [PMID:1311606]
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
  Sequence
Reference
6  [PMID:7656012]
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence
Reference
7  [PMID:9930981]
  Authors
Xia ZX, He YN, Dai WW, White SA, Boyd GD, Mathews FS
  Title
Detailed active site configuration of a new crystal form of methanol dehydrogenase from Methylophilus W3A1 at 1.9 A resolution.
  Journal
Biochemistry 38:1214-20 (1999)
DOI:10.1021/bi9822574
Reference
8  [PMID:11502173]
  Authors
Afolabi PR, Mohammed F, Amaratunga K, Majekodunmi O, Dales SL, Gill R, Thompson D, Cooper JB, Wood SP, Goodwin PM, Anthony C
  Title
Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).
  Journal
Biochemistry 40:9799-809 (2001)
DOI:10.1021/bi002932l
  Sequence
Reference
9  [PMID:12686102]
  Authors
Anthony C, Williams P
  Title
The structure and mechanism of methanol dehydrogenase.
  Journal
Biochim Biophys Acta 1647:18-23 (2003)
DOI:10.1016/S1570-9639(03)00042-6
Reference
10 [PMID:15608378]
  Authors
Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB
  Title
The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.
  Journal
Acta Crystallogr D Biol Crystallogr 61:75-9 (2005)
DOI:10.1107/S0907444904026964
Other DBs
ExplorEnz - The Enzyme Database: 1.1.2.7
IUBMB Enzyme Nomenclature: 1.1.2.7
ExPASy - ENZYME nomenclature database: 1.1.2.7
UM-BBD (Biocatalysis/Biodegradation Database): 1.1.2.7
BRENDA, the Enzyme Database: 1.1.2.7
CAS: 37205-43-9

KEGG   REACTION: R01146
Entry
R01146                      Reaction                               

Name
methanol:ferricytochrome-c oxidoreductase;
methanol dehydrogenase
Definition
Methanol + 2 Ferricytochrome cL <=> Formaldehyde + 2 Ferrocytochrome cL + 2 H+
Equation
C00132 + 2 C18233 <=> C00067 + 2 C18234 + 2 C00080
Reaction class
RC00034  C00067_C00132
Enzyme
1.1.2.7         1.1.2.10
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Orthology
K14028  methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
K14029  methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
K23995  lanthanide-dependent methanol dehydrogenase [EC:1.1.2.10]
Other DBs
RHEA: 51011

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