KEGG   ORTHOLOGY: K14082
Entry
K14082                      KO                                     

Name
mtbA
Definition
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K14082  mtbA; [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.247  [methyl-Co(III) methylamine-specific corrinoid protein]---coenzyme M methyltransferase
     K14082  mtbA; [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Other DBs
RN: R09124 R09998 R09999 R10000
COG: COG0407
Genes
MAH: MEALZ_1444
MBUR: EQU24_13400
CTHD: CDO33_16885
SGY: Sgly_0963 Sgly_2789
MAS: Mahau_2672
PUF: UFO1_2885
TTF: THTE_3705
ALUS: STSP2_03206(hemE_2)
VBL: L21SP4_02246
TAZ: TREAZ_1481 TREAZ_1866
MBAR: MSBR2_2229
MBAK: MSBR3_2280
MAC: MA_0146(mtbA)
MMA: MM_1439(mtbA)
MMAC: MSMAC_3247
MTHR: MSTHT_1928
MTHE: MSTHC_1355
MHOR: MSHOH_3972
MBU: Mbur_2082(mtbA)
MMET: MCMEM_0281
MMH: Mmah_0279
 » show all
Reference
PMID:8654414
  Authors
Harms U, Thauer RK
  Title
Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.
  Journal
Eur J Biochem 235:653-9 (1996)
DOI:10.1111/j.1432-1033.1996.00653.x
  Sequence
Reference
  Authors
Pritchett MA, Metcalf WW
  Title
Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.
  Journal
Mol Microbiol 56:1183-94 (2005)
DOI:10.1111/j.1365-2958.2005.04616.x

KEGG   ENZYME: 2.1.1.247
Entry
EC 2.1.1.247                Enzyme                                 

Name
[methyl-Co(III) methylamine-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
MT2 (ambiguous);
MT2-A;
mtbA (gene name);
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated monomethylamine-specific corrinoid protein:CoM methyltransferase
Reaction(IUBMB)
a [methyl-Co(III) methylamine-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylamine-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
R10000;
(other) R09124 R09998 R09999
Substrate
[methyl-Co(III) methylamine-specific corrinoid protein];
CoM [CPD:C03576]
Product
methyl-CoM [CPD:C03920];
[Co(I) methylamine-specific corrinoid protein]
Comment
Contains zinc [2]. The enzyme, which is involved in methanogenesis from mono-, di-, and trimethylamine, catalyses the transfer of a methyl group bound to the cobalt cofactor of several corrinoid proteins (mono-, di-, and trimethylamine-specific corrinoid proteins, cf. EC 2.1.1.248, methylamine---corrinoid protein Co-methyltransferase, EC 2.1.1.249, dimethylamine---corrinoid protein Co-methyltransferase, and EC 2.1.1.250, trimethylamine---corrinoid protein Co-methyltransferase) to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis.
History
EC 2.1.1.247 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Genes
MAH: MEALZ_1444
MBUR: EQU24_13400
CTHD: CDO33_16885
SGY: Sgly_0963 Sgly_2789
MAS: Mahau_2672
PUF: UFO1_2885
TTF: THTE_3705
ALUS: STSP2_03206(hemE_2)
VBL: L21SP4_02246
TAZ: TREAZ_1481 TREAZ_1866
MBAR: MSBR2_2229
MBAK: MSBR3_2280
MAC: MA_0146(mtbA)
MMA: MM_1439(mtbA)
MMAC: MSMAC_3247
MTHR: MSTHT_1928
MTHE: MSTHC_1355
MHOR: MSHOH_3972
MBU: Mbur_2082(mtbA)
MMET: MCMEM_0281
MMH: Mmah_0279
 » show all
Reference
1  [PMID:7635826]
  Authors
Burke SA, Krzycki JA
  Title
Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine.
  Journal
J Bacteriol 177:4410-6 (1995)
DOI:10.1128/JB.177.15.4410-4416.1995
  Sequence
Reference
2  [PMID:8702528]
  Authors
LeClerc GM, Grahame DA
  Title
Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.
  Journal
J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725
  Sequence
Reference
3  [PMID:9006042]
  Authors
Ferguson DJ Jr, Krzycki JA
  Title
Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri.
  Journal
J Bacteriol 179:846-52 (1997)
DOI:10.1128/JB.179.3.846-852.1997
  Sequence
Reference
4  [PMID:9642198]
  Authors
Burke SA, Lo SL, Krzycki JA
  Title
Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.
  Journal
J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998
  Sequence
Reference
5  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.247
IUBMB Enzyme Nomenclature: 2.1.1.247
ExPASy - ENZYME nomenclature database: 2.1.1.247
BRENDA, the Enzyme Database: 2.1.1.247

KEGG   REACTION: R09124
Entry
R09124                      Reaction                               

Name
trimethylamine:coenzyme M methyltransferase
Definition
Trimethylamine + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + Dimethylamine
Equation
Comment
methylcobamide:CoM methyltransferase: mtbA
trimethylamine methyltransferase: mttB
trimethylamine corrinoid protein: mttC
two-step reaction (see R10016+R10000)
Reaction class
RC00035  C03576_C03920
RC00732  C00543_C00565
RC02984  C00565_C03920
Enzyme
2.1.1.247       2.1.1.250
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
K14083  trimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.250]
K14084  trimethylamine corrinoid protein

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