KEGG   ORTHOLOGY: K14082
Entry
K14082                      KO                                     

Name
mtbA
Definition
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K14082  mtbA; [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.247  [methyl-Co(III) methylamine-specific corrinoid protein]---coenzyme M methyltransferase
     K14082  mtbA; [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Other DBs
RN: R09124 R09998 R09999 R10000
COG: COG0407
Genes
MAH: MEALZ_1444
MBUR: EQU24_13400
CTHD: CDO33_16885
SGY: Sgly_0963 Sgly_2789
MAS: Mahau_2672
PUF: UFO1_2885
TTF: THTE_3705
ALUS: STSP2_03206(hemE_2)
VBL: L21SP4_02246
TAZ: TREAZ_1481 TREAZ_1866
CPOR: BED41_14920
MBA: Mbar_A0841
MBY: MSBRM_0152
MBW: MSBRW_0160
MBAR: MSBR2_2229
MBAK: MSBR3_2280
MAC: MA_0146(mtbA)
MMA: MM_1439(mtbA)
MMAZ: MmTuc01_1497
MMJ: MSMAS_3225
MMAC: MSMAC_3247
MVC: MSVAZ_3363
MEK: MSKOL_3324
MLS: MSLAZ_3206
METM: MSMTP_0576 MSMTP_1917 MSMTP_3048 MSMTP_3126
MEF: MSWH1_3073
MEQ: MSWHS_3284
MSJ: MSSAC_0128
MSZ: MSSIH_0114
MSW: MSSIT_0118
MTHR: MSTHT_1928
MTHE: MSTHC_1355
MHOR: MSHOH_3972
MFZ: AOB57_008940
MBU: Mbur_2082(mtbA)
MMET: MCMEM_0281
MMH: Mmah_0279
MHAZ: BHR79_02125
MEV: Metev_0310
MZH: Mzhil_1020
MPY: Mpsy_1673 Mpsy_1705
MZI: HWN40_00845 HWN40_00915 HWN40_05355
MHZ: Metho_0007 Metho_0037 Metho_0355
MER: MMINT_09350
 » show all
Reference
PMID:8654414
  Authors
Harms U, Thauer RK
  Title
Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.
  Journal
Eur J Biochem 235:653-9 (1996)
DOI:10.1111/j.1432-1033.1996.00653.x
  Sequence
Reference
  Authors
Pritchett MA, Metcalf WW
  Title
Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.
  Journal
Mol Microbiol 56:1183-94 (2005)
DOI:10.1111/j.1365-2958.2005.04616.x

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Ontology (3)   
   KEGG BRITE (2)   
   COG (1)   
Pathway (9)   
   KEGG PATHWAY (8)   
   KEGG MODULE (1)   
Chemical reaction (12)   
   KEGG ENZYME (1)   
   KEGG REACTION (4)   
   KEGG RCLASS (7)   
Gene (57)   
   KEGG GENES (54)   
   OC (3)   
Literature (2)   
   PubMed (2)   
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KEGG   ENZYME: 2.1.1.247
Entry
EC 2.1.1.247                Enzyme                                 

Name
[methyl-Co(III) methylamine-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
MT2 (ambiguous);
MT2-A;
mtbA (gene name);
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated monomethylamine-specific corrinoid protein:CoM methyltransferase
Reaction(IUBMB)
a [methyl-Co(III) methylamine-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylamine-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
R10000;
(other) R09124 R09998 R09999
Substrate
[methyl-Co(III) methylamine-specific corrinoid protein];
CoM [CPD:C03576]
Product
methyl-CoM [CPD:C03920];
[Co(I) methylamine-specific corrinoid protein]
Comment
Contains zinc [2]. The enzyme, which is involved in methanogenesis from mono-, di-, and trimethylamine, catalyses the transfer of a methyl group bound to the cobalt cofactor of several corrinoid proteins (mono-, di-, and trimethylamine-specific corrinoid proteins, cf. EC 2.1.1.248, methylamine---corrinoid protein Co-methyltransferase, EC 2.1.1.249, dimethylamine---corrinoid protein Co-methyltransferase, and EC 2.1.1.250, trimethylamine---corrinoid protein Co-methyltransferase) to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis.
History
EC 2.1.1.247 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
Genes
MAH: MEALZ_1444
MBUR: EQU24_13400
CTHD: CDO33_16885
SGY: Sgly_0963 Sgly_2789
MAS: Mahau_2672
PUF: UFO1_2885
TTF: THTE_3705
ALUS: STSP2_03206(hemE_2)
VBL: L21SP4_02246
TAZ: TREAZ_1481 TREAZ_1866
CPOR: BED41_14920
MBA: Mbar_A0841
MBY: MSBRM_0152
MBW: MSBRW_0160
MBAR: MSBR2_2229
MBAK: MSBR3_2280
MAC: MA_0146(mtbA)
MMA: MM_1439(mtbA)
MMAZ: MmTuc01_1497
MMJ: MSMAS_3225
MMAC: MSMAC_3247
MVC: MSVAZ_3363
MEK: MSKOL_3324
MLS: MSLAZ_3206
METM: MSMTP_0576 MSMTP_1917 MSMTP_3048 MSMTP_3126
MEF: MSWH1_3073
MEQ: MSWHS_3284
MSJ: MSSAC_0128
MSZ: MSSIH_0114
MSW: MSSIT_0118
MTHR: MSTHT_1928
MTHE: MSTHC_1355
MHOR: MSHOH_3972
MFZ: AOB57_008940
MBU: Mbur_2082(mtbA)
MMET: MCMEM_0281
MMH: Mmah_0279
MHAZ: BHR79_02125
MEV: Metev_0310
MZH: Mzhil_1020
MPY: Mpsy_1673 Mpsy_1705
MZI: HWN40_00845 HWN40_00915 HWN40_05355
MHZ: Metho_0007 Metho_0037 Metho_0355
MER: MMINT_09350
 » show all
Reference
1  [PMID:7635826]
  Authors
Burke SA, Krzycki JA
  Title
Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine.
  Journal
J Bacteriol 177:4410-6 (1995)
DOI:10.1128/JB.177.15.4410-4416.1995
  Sequence
Reference
2  [PMID:8702528]
  Authors
LeClerc GM, Grahame DA
  Title
Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.
  Journal
J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725
  Sequence
Reference
3  [PMID:9006042]
  Authors
Ferguson DJ Jr, Krzycki JA
  Title
Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri.
  Journal
J Bacteriol 179:846-52 (1997)
DOI:10.1128/JB.179.3.846-852.1997
  Sequence
Reference
4  [PMID:9642198]
  Authors
Burke SA, Lo SL, Krzycki JA
  Title
Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.
  Journal
J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998
  Sequence
Reference
5  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.247
IUBMB Enzyme Nomenclature: 2.1.1.247
ExPASy - ENZYME nomenclature database: 2.1.1.247
BRENDA, the Enzyme Database: 2.1.1.247

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Chemical substance (8)   
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Chemical reaction (11)   
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Gene (55)   
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Protein sequence (36)   
   UniProt (29)   
   SWISS-PROT (3)   
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DNA sequence (2)   
   GenBank (1)   
   EMBL (1)   
3D Structure (2)   
   PDB (2)   
Protein domain (3)   
   InterPro (3)   
All databases (122)   

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KEGG   REACTION: R09124
Entry
R09124                      Reaction                               

Name
trimethylamine:coenzyme M methyltransferase
Definition
Trimethylamine + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + Dimethylamine
Equation
Comment
methylcobamide:CoM methyltransferase: mtbA
trimethylamine methyltransferase: mttB
trimethylamine corrinoid protein: mttC
two-step reaction (see R10016+R10000)
Reaction class
RC00035  C03576_C03920
RC00732  C00543_C00565
RC02984  C00565_C03920
Enzyme
2.1.1.247       2.1.1.250
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
K14083  trimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.250]
K14084  trimethylamine corrinoid protein

  All links  
Ontology (1)   
   KEGG BRITE (1)   
Pathway (9)   
   KEGG PATHWAY (8)   
   KEGG MODULE (1)   
Chemical substance (4)   
   KEGG COMPOUND (4)   
Chemical reaction (5)   
   KEGG ENZYME (2)   
   KEGG RCLASS (3)   
Gene (1128)   
   KEGG ORTHOLOGY (3)   
   KEGG GENES (1125)   
All databases (1147)   

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