KEGG   ORTHOLOGY: K14083
Entry
K14083                      KO                                     

Name
mttB
Definition
trimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.250]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K14083  mttB; trimethylamine---corrinoid protein Co-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.250  trimethylamine---corrinoid protein Co-methyltransferase
     K14083  mttB; trimethylamine---corrinoid protein Co-methyltransferase
Other DBs
RN: R09124 R10016
COG: COG5598
Genes
GAI: IMCC3135_27710
TSN: W908_00365 W908_00465 W908_00520 W908_00960 W908_08075
THIN: CRN91_00505 CRN91_00870 CRN91_00905 CRN91_02065
AZA: AZKH_p0144
AZD: CDA09_10875 CDA09_11025
DAK: DaAHT2_2485 DaAHT2_2487 DaAHT2_2574
DOL: Dole_1106 Dole_1109 Dole_1111
DML: Dmul_15240(mttB1) Dmul_16770(mttB2)
DAL: Dalk_4373
DAT: HRM2_00490(mttB1) HRM2_01250(mttB2) HRM2_07480(mttB3) HRM2_09120(mttB4) HRM2_15910(mttB5) HRM2_15930(mttB6) HRM2_44090(mttB7) HRM2_44130(mttB8) HRM2_44190(mttB9) HRM2_44210(mttB10) HRM2_45990(mttB711)
SFD: USDA257_c05310(mttB1) USDA257_c42530(mttB2) USDA257_c45720(mttB3)
NEN: NCHU2750_15830(mttB) NCHU2750_25080(mttB)
RSP: RSP_2813(mttB) RSP_4008
PAMN: pAMV3p0238
DSH: Dshi_1586(mttb) Dshi_2817
PGA: PGA1_c08570 PGA1_c14630(mttB1) PGA1_c16500(mttB2) PGA1_c25550(mttB3)
PGL: PGA2_c08310 PGA2_c14540(mttB1) PGA2_c16330(mttB2)
OAT: OAN307_c14400(mttB1) OAN307_c22520(mttB3) OAN307_c25200(mttB2)
RSU: NHU_02107
RHC: RGUI_0134
PALW: PSAL_010110(mtgB_1) PSAL_013210(mtgB_2)
CACE: CACET_c34470(mttB)
AWO: Awo_c05430(mttB3) Awo_c05440(mttB4) Awo_c05470(mttB5) Awo_c07540(mttB10) Awo_c27930(mttB21) Awo_c27940(mttB22)
STED: SPTER_03940(mtgB_1) SPTER_03990(mtgB_2) SPTER_06970(mtgB_3) SPTER_09870(mtgB_4) SPTER_39430(mtgB_5)
MBAR: MSBR2_1920
MBAK: MSBR3_1951
MAC: MA_0528(mttB) MA_0932(mttB)
MMA: MM_1688(mttB) MM_1689(mttB) MM_2048(mttB) MM_2049(mttB)
MHOR: MSHOH_3689
MBU: Mbur_2308 Mbur_2313(mttB)
MMET: MCMEM_1860
MPY: Mpsy_1688(mttB2)
HHSR: HSR6_1744 HSR6_1804(mttB)
HDF: AArcSl_0468(mttB) AArcSl_1175(mttB2)
MARC: AR505_0772
TPE: Tpen_1467
LOKI: Lokiarch_24540(mttB)
AG: AAD14630(mttB)
 » show all
Reference
  Authors
Pritchett MA, Metcalf WW
  Title
Genetic, physiological and biochemical characterization of multiple methanol methyltransferase isozymes in Methanosarcina acetivorans C2A.
  Journal
Mol Microbiol 56:1183-94 (2005)
DOI:10.1111/j.1365-2958.2005.04616.x

KEGG   ENZYME: 2.1.1.250
Entry
EC 2.1.1.250                Enzyme                                 

Name
trimethylamine---corrinoid protein Co-methyltransferase;
mttB (gene name);
trimethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
trimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] = a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine [RN:R10016]
Reaction(KEGG)
R10016;
(other) R09124
Substrate
trimethylamine [CPD:C00565];
[Co(I) trimethylamine-specific corrinoid protein]
Product
[methyl-Co(III) trimethylamine-specific corrinoid protein];
dimethylamine [CPD:C00543]
Comment
The enzyme, which catalyses the transfer of a methyl group from trimethylamine to a trimethylamine-specific corrinoid protein (MttC), is involved in methanogenesis from trimethylamine. The enzyme contains the unusual amino acid pyrrolysine [2]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.250 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14083  trimethylamine---corrinoid protein Co-methyltransferase
Genes
GAI: IMCC3135_27710
TSN: W908_00365 W908_00465 W908_00520 W908_00960 W908_08075
THIN: CRN91_00505 CRN91_00870 CRN91_00905 CRN91_02065
AZA: AZKH_p0144
AZD: CDA09_10875 CDA09_11025
DAK: DaAHT2_2485 DaAHT2_2487 DaAHT2_2574
DOL: Dole_1106 Dole_1109 Dole_1111
DML: Dmul_15240(mttB1) Dmul_16770(mttB2)
DAL: Dalk_4373
DAT: HRM2_00490(mttB1) HRM2_01250(mttB2) HRM2_07480(mttB3) HRM2_09120(mttB4) HRM2_15910(mttB5) HRM2_15930(mttB6) HRM2_44090(mttB7) HRM2_44130(mttB8) HRM2_44190(mttB9) HRM2_44210(mttB10) HRM2_45990(mttB711)
SFD: USDA257_c05310(mttB1) USDA257_c42530(mttB2) USDA257_c45720(mttB3)
NEN: NCHU2750_15830(mttB) NCHU2750_25080(mttB)
RSP: RSP_2813(mttB) RSP_4008
PAMN: pAMV3p0238
DSH: Dshi_1586(mttb) Dshi_2817
PGA: PGA1_c08570 PGA1_c14630(mttB1) PGA1_c16500(mttB2) PGA1_c25550(mttB3)
PGL: PGA2_c08310 PGA2_c14540(mttB1) PGA2_c16330(mttB2)
OAT: OAN307_c14400(mttB1) OAN307_c22520(mttB3) OAN307_c25200(mttB2)
RSU: NHU_02107
RHC: RGUI_0134
PALW: PSAL_010110(mtgB_1) PSAL_013210(mtgB_2)
CACE: CACET_c34470(mttB)
AWO: Awo_c05430(mttB3) Awo_c05440(mttB4) Awo_c05470(mttB5) Awo_c07540(mttB10) Awo_c27930(mttB21) Awo_c27940(mttB22)
STED: SPTER_03940(mtgB_1) SPTER_03990(mtgB_2) SPTER_06970(mtgB_3) SPTER_09870(mtgB_4) SPTER_39430(mtgB_5)
MBAR: MSBR2_1920
MBAK: MSBR3_1951
MAC: MA_0528(mttB) MA_0932(mttB)
MMA: MM_1688(mttB) MM_1689(mttB) MM_2048(mttB) MM_2049(mttB)
MHOR: MSHOH_3689
MBU: Mbur_2308 Mbur_2313(mttB)
MMET: MCMEM_1860
MPY: Mpsy_1688(mttB2)
HHSR: HSR6_1744 HSR6_1804(mttB)
HDF: AArcSl_0468(mttB) AArcSl_1175(mttB2)
MARC: AR505_0772
TPE: Tpen_1467
LOKI: Lokiarch_24540(mttB)
 » show all
Reference
1  [PMID:9006042]
  Authors
Ferguson DJ Jr, Krzycki JA
  Title
Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri.
  Journal
J Bacteriol 179:846-52 (1997)
DOI:10.1128/JB.179.3.846-852.1997
  Sequence
Reference
2  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.250
IUBMB Enzyme Nomenclature: 2.1.1.250
ExPASy - ENZYME nomenclature database: 2.1.1.250
BRENDA, the Enzyme Database: 2.1.1.250

KEGG   REACTION: R09124
Entry
R09124                      Reaction                               

Name
trimethylamine:coenzyme M methyltransferase
Definition
Trimethylamine + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + Dimethylamine
Equation
Comment
methylcobamide:CoM methyltransferase: mtbA
trimethylamine methyltransferase: mttB
trimethylamine corrinoid protein: mttC
two-step reaction (see R10016+R10000)
Reaction class
RC00035  C03576_C03920
RC00732  C00543_C00565
RC02984  C00565_C03920
Enzyme
2.1.1.247       2.1.1.250
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
K14083  trimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.250]
K14084  trimethylamine corrinoid protein

DBGET integrated database retrieval system