KEGG   ORTHOLOGY: K14252
Entry
K14252                      KO                                     

Name
oxyL
Definition
6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase [EC:1.14.13.232 1.14.13.233]
Pathway
ko00253  Tetracycline biosynthesis
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00780  Tetracycline/oxytetracycline biosynthesis, pretetramide => tetracycline/oxytetracycline
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K14252  oxyL; 6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
   00253 Tetracycline biosynthesis
    K14252  oxyL; 6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.232  6-methylpretetramide 4-monooxygenase
     K14252  oxyL; 6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
    1.14.13.233  4-hydroxy-6-methylpretetramide 12a-monooxygenase
     K14252  oxyL; 6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
Other DBs
RN: R05462 R09190
COG: COG0654
Genes
NAH: F5544_10695
SBH: SBI_06866
SHY: SHJG_1193
SHO: SHJGH_1028
SCI: B446_13470
SPRI: SPRI_0201 SPRI_7152
SPAV: Spa2297_32760
SGS: AVL59_46185
KAU: B6264_18580
AORI: SD37_34185
KAL: KALB_7834
AG: AAZ78335(oxyL)
 » show all
Reference
  Authors
Zhang W, Ames BD, Tsai SC, Tang Y
  Title
Engineered biosynthesis of a novel amidated polyketide, using the malonamyl-specific initiation module from the oxytetracycline polyketide synthase.
  Journal
Appl Environ Microbiol 72:2573-80 (2006)
DOI:10.1128/AEM.72.4.2573-2580.2006
  Sequence
Reference
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Reference
  Authors
Wang P, Zhang W, Zhan J, Tang Y
  Title
Identification of OxyE as an ancillary oxygenase during tetracycline biosynthesis.
  Journal
Chembiochem 10:1544-50 (2009)
DOI:10.1002/cbic.200900122

KEGG   ENZYME: 1.14.13.233
Entry
EC 1.14.13.233              Enzyme                                 

Name
4-hydroxy-6-methylpretetramide 12a-monooxygenase;
oxyL (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
4-hydroxy-6-methylpretetramide,NADPH:oxygen oxidoreductase (12a-hydroxylating)
Reaction(IUBMB)
4-hydroxy-6-methylpretetramide + NADPH + H+ + O2 = 4-de(dimethylamino)-4-oxoanhydrotetracycline + NADP+ + H2O [RN:R05462]
Reaction(KEGG)
R05462;
(other) R09190
Substrate
4-hydroxy-6-methylpretetramide [CPD:C06628];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-de(dimethylamino)-4-oxoanhydrotetracycline [CPD:C06627];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Contains FAD. The enzyme, characterized from the bacterium Streptomyces rimosus, participates in the biosynthesis of tetracycline antibiotics. The enzyme is bifunctional, and can also catalyse EC 1.14.13.232, 6-methylpretetramide 4-monooxygenase.
History
EC 1.14.13.233 created 2016
Pathway
ec00253  Tetracycline biosynthesis
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14252  6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase
Genes
NAH: F5544_10695
SBH: SBI_06866
SHY: SHJG_1193
SHO: SHJGH_1028
SCI: B446_13470
SPRI: SPRI_0201 SPRI_7152
SPAV: Spa2297_32760
SGS: AVL59_46185
KAU: B6264_18580
AORI: SD37_34185
KAL: KALB_7834
 » show all
Reference
1  [PMID:18422316]
  Authors
Zhang W, Watanabe K, Cai X, Jung ME, Tang Y, Zhan J.
  Title
Identifying the minimal enzymes required for anhydrotetracycline biosynthesis.
  Journal
J Am Chem Soc 130:6068-9 (2008)
DOI:10.1021/ja800951e
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.233
IUBMB Enzyme Nomenclature: 1.14.13.233
ExPASy - ENZYME nomenclature database: 1.14.13.233
BRENDA, the Enzyme Database: 1.14.13.233

KEGG   REACTION: R05462
Entry
R05462                      Reaction                               

Name
4-hydroxy-6-methylpretetramide,NADPH:oxygen oxidoreductase (12a-hydroxylating)
Definition
4-Hydroxy-6-methylpretetramide + NADPH + Oxygen + H+ <=> 4-Keto-anhydrotetracycline + NADP+ + H2O
Equation
Comment
following after R05463
Reaction class
RC00001  C00005_C00006
RC01388  C06627_C06628
Enzyme
Pathway
rn00253  Tetracycline biosynthesis
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00780  Tetracycline/oxytetracycline biosynthesis, pretetramide => tetracycline/oxytetracycline
M00823  Chlortetracycline biosynthesis, pretetramide => chlortetracycline
Orthology
K14252  6-methylpretetramide 4-monooxygenase / 4-hydroxy-6-methylpretetramide 12a-monooxygenase [EC:1.14.13.232 1.14.13.233]
Other DBs
RHEA: 50359

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