KEGG   ORTHOLOGY: K14367Help
Entry
K14367                      KO                                     

Name
eryBV
Definition
erythronolide mycarosyltransferase [EC:2.4.1.328]
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01052  Type I polyketide structures
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K14367  eryBV; erythronolide mycarosyltransferase
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K14367  eryBV; erythronolide mycarosyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14367  eryBV; erythronolide mycarosyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.328  erythronolide mycarosyltransferase
     K14367  eryBV; erythronolide mycarosyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Glycosyltransferase
   K14367  eryBV; erythronolide mycarosyltransferase
BRITE hierarchy
Other DBs
RN: R05530
CAZy: GT1
Genes
SEN: SACE_0719(eryBV)
AEY: CDG81_13195
AG: AAG13915(megBV)
TaxonomyKoalaUniProt
Reference
PMID:9353926
  Authors
Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L.
  Title
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
  Journal
Microbiology 143:3251-62 (1997)
DOI:10.1099/00221287-143-10-3251
  Sequence
[sen:SACE_0719]
Reference
PMID:9393448
  Authors
Gaisser S, Bohm GA, Cortes J, Leadlay PF
  Title
Analysis of seven genes from the eryAI-eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea.
  Journal
Mol Gen Genet 256:239-51 (1997)
DOI:10.1007/s004380050566

KEGG   ENZYME: 2.4.1.328Help
Entry
EC 2.4.1.328                Enzyme                                 

Name
erythronolide mycarosyltransferase;
EryBV
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dTDP-beta-L-mycarose:erythronolide B L-mycarosyltransferase
Reaction(IUBMB)
dTDP-beta-L-mycarose + erythronolide B = dTDP + 3-alpha-L-mycarosylerythronolide B [RN:R05530]
Reaction(KEGG)
Substrate
dTDP-beta-L-mycarose [CPD:C11916];
erythronolide B [CPD:C06635]
Product
dTDP [CPD:C00363];
3-alpha-L-mycarosylerythronolide B [CPD:C06630]
Comment
Isolated from the bacterium Saccharopolyspora erythraea. The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
History
EC 2.4.1.328 created 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K14367  erythronolide mycarosyltransferase
Genes
SEN: SACE_0719(eryBV)
AEY: CDG81_13195
Taxonomy
Reference
1  [PMID:17262863]
  Authors
Zhang C, Fu Q, Albermann C, Li L, Thorson JS
  Title
The in vitro characterization of the erythronolide mycarosyltransferase EryBV and its utility in macrolide diversification.
  Journal
Chembiochem 8:385-90 (2007)
DOI:10.1002/cbic.200600509
  Sequence
[sen:SACE_0719]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.328
IUBMB Enzyme Nomenclature: 2.4.1.328
ExPASy - ENZYME nomenclature database: 2.4.1.328
BRENDA, the Enzyme Database: 2.4.1.328

KEGG   REACTION: R05530Help
Entry
R05530                      Reaction                               

Name
dTDP-L-mycarose:erythronolide-B 3-O-alpha-mycarosyltransferase
Definition
Erythronolide B + dTDP-L-mycarose <=> 3-O-alpha-Mycarosylerythronolide B + dTDP
Equation
Reaction class
RC00005  C00363_C11916
RC00049  C06630_C06635
RC03284  C06630_C11916
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Orthology
K14367  erythronolide mycarosyltransferase [EC:2.4.1.328]
Other DBs
RHEA: 41579

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