| Entry |
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| Name |
erythromycin 3''-O-methyltransferase;
EryG
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase
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| Reaction(IUBMB) |
(1) S-adenosyl-L-methionine + erythromycin C = S-adenosyl-L-homocysteine + erythromycin A [RN: R05520];
(2) S-adenosyl-L-methionine + erythromycin D = S-adenosyl-L-homocysteine + erythromycin B [RN: R05532]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
erythromycin C [CPD: C06616];
erythromycin D [CPD: C06633]
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
erythromycin A [CPD: C01912];
erythromycin B [CPD: C06653]
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| Comment |
The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC 1.14.13.154, erythromycin 12 hydroxylase.
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| History |
EC 2.1.1.254 created 2012
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| Pathway |
| ec00522 | Biosynthesis of 12-, 14- and 16-membered macrolides |
| ec01130 | Biosynthesis of antibiotics |
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| Orthology |
| K14369 | erythromycin 3''-O-methyltransferase |
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| Genes |
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| Reference |
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| Authors |
Paulus TJ, Tuan JS, Luebke VE, Maine GT, DeWitt JP, Katz L |
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| Title |
Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L. |
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| Title |
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExPASy - ENZYME nomenclature database: | 2.1.1.254 |
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