KEGG   ORTHOLOGY: K14369Help
Entry
K14369                      KO                                     

Name
eryG
Definition
erythromycin 3''-O-methyltransferase [EC:2.1.1.254]
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K14369  eryG; erythromycin 3''-O-methyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14369  eryG; erythromycin 3''-O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.254  erythromycin 3''-O-methyltransferase
     K14369  eryG; erythromycin 3''-O-methyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Methylase
   K14369  eryG; erythromycin 3''-O-methyltransferase
BRITE hierarchy
Other DBs
RN: R05520 R05532
Genes
AER: AERYTH_15890
SEN: SACE_0728(eryG)
TaxonomyKoalaUniProt
Reference
PMID:1840640
  Authors
Haydock SF, Dowson JA, Dhillon N, Roberts GA, Cortes J, Leadlay PF
  Title
Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases.
  Journal
Mol Gen Genet 230:120-8 (1991)
DOI:10.1007/BF00290659
  Sequence
[sen:SACE_0728]

KEGG   ENZYME: 2.1.1.254Help
Entry
EC 2.1.1.254                Enzyme                                 

Name
erythromycin 3''-O-methyltransferase;
EryG
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + erythromycin C = S-adenosyl-L-homocysteine + erythromycin A [RN:R05520];
(2) S-adenosyl-L-methionine + erythromycin D = S-adenosyl-L-homocysteine + erythromycin B [RN:R05532]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
erythromycin C [CPD:C06616];
erythromycin D [CPD:C06633]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
erythromycin A [CPD:C01912];
erythromycin B [CPD:C06653]
Comment
The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC 1.14.13.154, erythromycin 12 hydroxylase.
History
EC 2.1.1.254 created 2012
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K14369  erythromycin 3''-O-methyltransferase
Genes
AER: AERYTH_15890
SEN: SACE_0728(eryG)
Taxonomy
Reference
1  [PMID:2185226]
  Authors
Paulus TJ, Tuan JS, Luebke VE, Maine GT, DeWitt JP, Katz L
  Title
Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli.
  Journal
J Bacteriol 172:2541-6 (1990)
DOI:10.1128/JB.172.5.2541-2546.1990
  Sequence
[sen:SACE_0728]
Reference
2  [PMID:9353926]
  Authors
Summers RG, Donadio S, Staver MJ, Wendt-Pienkowski E, Hutchinson CR, Katz L.
  Title
Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
  Journal
Microbiology 143 ( Pt 10):3251-62 (1997)
DOI:10.1099/00221287-143-10-3251
  Sequence
[sen:SACE_0728]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.254
IUBMB Enzyme Nomenclature: 2.1.1.254
ExPASy - ENZYME nomenclature database: 2.1.1.254
BRENDA, the Enzyme Database: 2.1.1.254

KEGG   REACTION: R05520Help
Entry
R05520                      Reaction                               

Name
S-adenosyl-L-methionine:erythromycin-C 3''-O-methyltransferase
Definition
S-Adenosyl-L-methionine + Erythromycin C <=> S-Adenosyl-L-homocysteine + Erythromycin
Equation
Comment
EryG: O-methyltransferase
Reaction class
RC00003  C00019_C00021
RC01324  C01912_C06616
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00774  Erythromycin biosynthesis, propanoyl-CoA + methylmalonyl-CoA => deoxyerythronolide B => erythromycin A/B
Orthology
K14369  erythromycin 3''-O-methyltransferase [EC:2.1.1.254]
Other DBs
RHEA: 32650

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