KEGG   ORTHOLOGY: K14630Help
Entry
K14630                      KO                                     

Name
actVA5
Definition
two-component flavin-dependent monooxygenase [EC:1.14.14.-]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K14630  actVA5; two-component flavin-dependent monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14630  actVA5; two-component flavin-dependent monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.-  
     K14630  actVA5; two-component flavin-dependent monooxygenase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Oxygenase
   K14630  actVA5; two-component flavin-dependent monooxygenase
BRITE hierarchy
Other DBs
RN: R09313 R09314
Genes
SCO: SCO5080(actVA5)
SDV: BN159_2499
SLV: SLIV_12985
SVT: SVTN_18835
TFU: Tfu_1234
FRA: Francci3_4102
FRI: FraEuI1c_1309
AMS: AMIS_50490
CAI: Caci_6489
TaxonomyKoalaUniProt
Reference
  Authors
Valton J, Filisetti L, Fontecave M, Niviere V
  Title
A two-component flavin-dependent monooxygenase involved in actinorhodin biosynthesis in Streptomyces coelicolor.
  Journal
J Biol Chem 279:44362-9 (2004)
DOI:10.1074/jbc.M407722200
Reference
  Authors
Valton J, Fontecave M, Douki T, Kendrew SG, Niviere V
  Title
An aromatic hydroxylation reaction catalyzed by a two-component FMN-dependent Monooxygenase. The ActVA-ActVB system from Streptomyces coelicolor.
  Journal
J Biol Chem 281:27-35 (2006)
DOI:10.1074/jbc.M506146200
Reference
  Authors
Ellis HR
  Title
The FMN-dependent two-component monooxygenase systems.
  Journal
Arch Biochem Biophys 497:1-12 (2010)
DOI:10.1016/j.abb.2010.02.007

KEGG   ORTHOLOGY: K14631Help
Entry
K14631                      KO                                     

Name
actVB
Definition
flavin reductase ActVB [EC:1.5.1.-]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K14631  actVB; flavin reductase ActVB
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.-  
     K14631  actVB; flavin reductase ActVB
BRITE hierarchy
Other DBs
RN: R05705
Genes
BUO: BRPE64_CCDS02020
BUE: BRPE67_CCDS03520
CID: P73_0185
PABY: Ga0080574_TMP226
NOV: TQ38_005245
NNO: NONO_c51280
NOZ: DMB37_03455
ROP: ROP_46610
RAV: AAT18_00630
SCO: SCO5092(actVB)
SHY: SHJG_7334
SLV: SLIV_12925(actVB)
SLE: sle_03080(sle_03080)
STRD: NI25_26845
SALF: SMD44_00656(actVB)
SALJ: SMD11_0681(actVB) SMD11_1393(actVB)
SLX: SLAV_40520(snaC2)
SFK: KY5_7707c
NDK: I601_2566(snaC)
ASE: ACPL_2178(ntaB) ACPL_6076
CAI: Caci_6495
TBI: Tbis_2517
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Valton J, Filisetti L, Fontecave M, Niviere V
  Title
A two-component flavin-dependent monooxygenase involved in actinorhodin biosynthesis in Streptomyces coelicolor.
  Journal
J Biol Chem 279:44362-9 (2004)
DOI:10.1074/jbc.M407722200
Reference
  Authors
Valton J, Fontecave M, Douki T, Kendrew SG, Niviere V
  Title
An aromatic hydroxylation reaction catalyzed by a two-component FMN-dependent Monooxygenase. The ActVA-ActVB system from Streptomyces coelicolor.
  Journal
J Biol Chem 281:27-35 (2006)
DOI:10.1074/jbc.M506146200
Reference
  Authors
Ellis HR
  Title
The FMN-dependent two-component monooxygenase systems.
  Journal
Arch Biochem Biophys 497:1-12 (2010)
DOI:10.1016/j.abb.2010.02.007
Reference
  Authors
Filisetti L, Fontecave M, Niviere V
  Title
Mechanism and substrate specificity of the flavin reductase ActVB from Streptomyces coelicolor.
  Journal
J Biol Chem 278:296-303 (2003)
DOI:10.1074/jbc.M209689200

KEGG   ORTHOLOGY: K14632Help
Entry
K14632                      KO                                     

Name
actVA6
Definition
C-6 monooxygenase
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K14632  actVA6; C-6 monooxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K14632  actVA6; C-6 monooxygenase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Oxygenase
   K14632  actVA6; C-6 monooxygenase
BRITE hierarchy
Other DBs
RN: R06696
Genes
SCO: SCO5081(actVA6)
SLV: SLIV_12980
SRO: Sros_4752
AOI: AORI_4199
AJA: AJAP_17915
CAI: Caci_6490
TaxonomyKoalaUniProt
Reference
PMID:9209048
  Authors
Kendrew SG, Hopwood DA, Marsh EN
  Title
Identification of a monooxygenase from Streptomyces coelicolor A3(2) involved in biosynthesis of actinorhodin: purification and characterization of the recombinant enzyme.
  Journal
J Bacteriol 179:4305-10 (1997)
DOI:10.1128/JB.179.13.4305-4310.1997
Reference
  Authors
Sciara G, Kendrew SG, Miele AE, Marsh NG, Federici L, Malatesta F, Schimperna G, Savino C, Vallone B
  Title
The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.
  Journal
EMBO J 22:205-15 (2003)
DOI:10.1093/emboj/cdg031

KEGG   REACTION: R06696Help
Entry
R06696                      Reaction                               

Definition
6-Deoxydihydrokalafungin + Oxygen <=> Dihydrokalafungin + H2O
Equation
Comment
cofactor-independent monooxygenase, actVA-orf6
Reaction class
RC01675  C12435_C12436
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Orthology
K14632  C-6 monooxygenase

KEGG   REACTION: R09314Help
Entry
R09314                      Reaction                               

Definition
6-Deoxydihydrokalafungin + Reduced FMN + Oxygen <=> Dihydrokalafungin + FMN + H2O
Equation
Comment
C6 oxygenation, FMN-dependent monooxygenase
Reaction class
RC00126  C00061_C01847
RC01675  C12435_C12436
Enzyme
1.14.14.-
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Orthology
K14630  two-component flavin-dependent monooxygenase [EC:1.14.14.-]

KEGG   REACTION: R05705Help
Entry
R05705                      Reaction                               

Name
FMNH2:NAD+ oxidoreductase
Definition
Reduced FMN + NAD+ <=> FMN + NADH + H+
Equation
Comment
see R05706 (NADPH)
Reaction class
RC00001  C00003_C00004
RC00126  C00061_C01847
Enzyme
1.5.1.36        1.5.1.39        1.5.1.41        1.5.1.42        
1.5.1.-
Pathway
rn00740  Riboflavin metabolism
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00779  Dihydrokalafungin biosynthesis, octaketide => dihydrokalafungin
Orthology
K00484  flavin reductase (NADH) [EC:1.5.1.36]
K05368  aquacobalamin reductase / NAD(P)H-flavin reductase [EC:1.16.1.3 1.5.1.41]
K13562  NAD(P)H:FMN oxidoreductase [EC:1.5.1.-]
K14631  flavin reductase ActVB [EC:1.5.1.-]
K19286  FMN reductase [NAD(P)H] [EC:1.5.1.39]
K20146  nitrilotriacetate monooxygenase reductase component [EC:1.5.1.42]
K22393  flavin reductase (NADH) subunit 1 [EC:1.5.1.36]
K22394  flavin reductase (NADH) subunit 2 [EC:1.5.1.36]
K23462  flavin-dependent trigonelline monooxygenase, reductase component [EC:1.5.1.36]
K23470  4-hydroxyphenylacetate 3-hydroxylase, reductase component [EC:1.5.1.36]
Other DBs
RHEA: 21623

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