KEGG   ORTHOLOGY: K15314
Entry
K15314                      KO                                     

Name
sgcE, mdpE, ncsE, kedE, calE8, pksE
Definition
enediyne polyketide synthase
Pathway
ko01059  Biosynthesis of enediyne antibiotics
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00824  9-membered enediyne core biosynthesis, malonyl-CoA => 3-hydroxyhexadeca-4,6,8,10,12,14-hexaenoyl-ACP => 9-membered enediyne core
M00825  10-membered enediyne core biosynthesis, malonyl-CoA => 3-hydroxyhexadeca-4,6,8,10,12,14-hexaenoyl-ACP => 10-membered enediyne core
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01059 Biosynthesis of enediyne antibiotics
    K15314  sgcE, mdpE, ncsE, kedE, calE8, pksE; enediyne polyketide synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15314  sgcE, mdpE, ncsE, kedE, calE8, pksE; enediyne polyketide synthase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide synthase (PKS)
  Iterative type I PKS
   Bacterial type
    K15314  sgcE, mdpE, ncsE, kedE, calE8, pksE; enediyne polyketide synthase
Other DBs
RN: R11435
COG: COG0331 COG3321 COG1028
Genes
MCA: MCA1238
METU: GNH96_09805
MAH: MEALZ_1605
MBUR: EQU24_12390
TIG: THII_0012
REV: HUE57_15315
POX: MB84_31255
DOV: DSCO28_52900
DWD: DSCW_50130
DALK: DSCA_31660
ADE: Adeh_3899
HOH: Hoch_0693
RER: RER_03770
REY: O5Y_01845
SGR: SGR_609(pksE)
SGB: WQO_33990
STRM: M444_38005
AMD: AMED_6864
AMN: RAM_35220
AMM: AMES_6759
AMZ: B737_6759
AOI: AORI_1840
AMYY: YIM_40785(ppsA6)
AORI: SD37_32010
AMI: Amir_4083
SESP: BN6_39290(pks6-2)
ACTI: UA75_19075
ACAD: UA74_18585
AHG: AHOG_16585(ppsA)
ALO: CRK57498
MIL: ML5_5849
ACTN: L083_3210(pks1C)
CAI: Caci_6351
MAR: MAE_12840
MPK: VL20_1920
HAU: Haur_0866
AG: AAM78012(ncsE) AAM94794(calE8) AAQ17110(mdpE) AFV52145(kedE)
 » show all
Reference
  Authors
Horsman GP, Chen Y, Thorson JS, Shen B
  Title
Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes.
  Journal
Proc Natl Acad Sci U S A 107:11331-5 (2010)
DOI:10.1073/pnas.1003442107
Reference
  Authors
Horsman GP, Van Lanen SG, Shen B
  Title
Iterative type I polyketide synthases for enediyne core biosynthesis.
  Journal
Methods Enzymol 459:97-112 (2009)
DOI:10.1016/S0076-6879(09)04605-9
Reference
PMID:12183628 (sgcE)
  Authors
Liu W, Christenson SD, Standage S, Shen B
  Title
Biosynthesis of the enediyne antitumor antibiotic C-1027.
  Journal
Science 297:1170-3 (2002)
DOI:10.1126/science.1072110
  Sequence
[sgb:WQO_33990]
Reference
PMID:17918933 (mdpE)
  Authors
Van Lanen SG, Oh TJ, Liu W, Wendt-Pienkowski E, Shen B
  Title
Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis.
  Journal
J Am Chem Soc 129:13082-94 (2007)
DOI:10.1021/ja073275o
  Sequence
Reference
PMID:15797213 (ncsE)
  Authors
Liu W, Nonaka K, Nie L, Zhang J, Christenson SD, Bae J, Van Lanen SG, Zazopoulos E, Farnet CM, Yang CF, Shen B
  Title
The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases.
  Journal
Chem Biol 12:293-302 (2005)
DOI:10.1016/j.chembiol.2004.12.013
  Sequence
Reference
PMID:23360970 (kedE)
  Authors
Lohman JR, Huang SX, Horsman GP, Dilfer PE, Huang T, Chen Y, Wendt-Pienkowski E, Shen B
  Title
Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics.
  Journal
Mol Biosyst 9:478-91 (2013)
DOI:10.1039/c3mb25523a
  Sequence
Reference
PMID:12183629 (calE8)
  Authors
Ahlert J, Shepard E, Lomovskaya N, Zazopoulos E, Staffa A, Bachmann BO, Huang K, Fonstein L, Czisny A, Whitwam RE, Farnet CM, Thorson JS
  Title
The calicheamicin gene cluster and its iterative type I enediyne PKS.
  Journal
Science 297:1173-6 (2002)
DOI:10.1126/science.1072105
  Sequence

KEGG   REACTION: R11435
Entry
R11435                      Reaction                               

Definition
8 Malonyl-CoA + 7 NADPH + 7 H+ + Acyl-carrier protein <=> 3-Hydroxyhexadeca-4,6,8,10,12,14-hexaenoyl-[acp] + 8 CoA + 8 CO2 + 6 H2O + 7 NADP+
Equation
8 C00083 + 7 C00005 + 7 C00080 + C00229 <=> C21357 + 8 C00010 + 8 C00011 + 6 C00001 + 7 C00006
Pathway
rn01059  Biosynthesis of enediyne antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K15314  enediyne polyketide synthase

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