KEGG   ORTHOLOGY: K15316Help
Entry
K15316                      KO                                     

Name
PKSA
Definition
noranthrone synthase [EC:2.3.1.221]
Pathway
ko00254  Aflatoxin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00254 Aflatoxin biosynthesis
    K15316  PKSA; noranthrone synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15316  PKSA; noranthrone synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.221  noranthrone synthase
     K15316  PKSA; noranthrone synthase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide synthase (PKS)
  Iterative type I PKS
   Fungal nonreducing PKS (NR-PKS)
    K15316  PKSA; noranthrone synthase
BRITE hierarchy
Other DBs
RN: R10239
Genes
PAN: PODANSg8570
GLZ: GLAREA_01150
ANI: AN7825.2
AOR: AO090026000009
AFV: AFLA_139410
CIM: CIMG_08569
CPW: CPC735_062240
ABE: ARB_07994
TVE: TRV_04611
BCOM: BAUCODRAFT_62024
TaxonomyKoalaUniProt
Reference
PMID:7642507
  Authors
Yu JH, Leonard TJ
  Title
Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I polyketide synthase.
  Journal
J Bacteriol 177:4792-800 (1995)
DOI:10.1128/JB.177.16.4792-4800.1995
Reference
  Authors
Cox RJ
  Title
Polyketides, proteins and genes in fungi: programmed nano-machines begin to reveal their secrets.
  Journal
Org Biomol Chem 5:2010-26 (2007)
DOI:10.1039/b704420h

KEGG   ENZYME: 2.3.1.221Help
Entry
EC 2.3.1.221                Enzyme                                 

Name
noranthrone synthase;
polyketide synthase A (ambiguous);
PksA (ambiguous);
norsolorinic acid anthrone synthase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
malonyl-CoA:hexanoate malonyltransferase (norsolorinic acid anthrone-forming)
Reaction(IUBMB)
7 malonyl-CoA + hexanoyl-[acyl-carrier protein] = 7 CoA + norsolorinic acid anthrone + [acyl-carrier protein] + 7 CO2 + 2 H2O [RN:R10239]
Reaction(KEGG)
Substrate
malonyl-CoA [CPD:C00083];
hexanoyl-[acyl-carrier protein] [CPD:C05749]
Product
CoA [CPD:C00010];
norsolorinic acid anthrone [CPD:C20453];
[acyl-carrier protein] [CPD:C00229];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
A multi-domain polyketide synthase involved in the synthesis of aflatoxins in the fungus Aspergillus parasiticus. The hexanoyl starter unit is provided to the acyl-carrier protein (ACP) domain by a dedicated fungal fatty acid synthase [1].
History
EC 2.3.1.221 created 2013
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K15316  noranthrone synthase
Genes
PAN: PODANSg8570
GLZ: GLAREA_01150
ANI: AN7825.2
AOR: AO090026000009
AFV: AFLA_139410
CIM: CIMG_08569
CPW: CPC735_062240
ABE: ARB_07994
TVE: TRV_04611
BCOM: BAUCODRAFT_62024
Taxonomy
Reference
1  [PMID:18403714]
  Authors
Crawford JM, Thomas PM, Scheerer JR, Vagstad AL, Kelleher NL, Townsend CA
  Title
Deconstruction of iterative multidomain polyketide synthase function.
  Journal
Science 320:243-6 (2008)
DOI:10.1126/science.1154711
Reference
2  [PMID:19847268]
  Authors
Crawford JM, Korman TP, Labonte JW, Vagstad AL, Hill EA, Kamari-Bidkorpeh O, Tsai SC, Townsend CA
  Title
Structural basis for biosynthetic programming of fungal aromatic polyketide cyclization.
  Journal
Nature 461:1139-43 (2009)
DOI:10.1038/nature08475
Reference
3  [PMID:20332208]
  Authors
Korman TP, Crawford JM, Labonte JW, Newman AG, Wong J, Townsend CA, Tsai SC
  Title
Structure and function of an iterative polyketide synthase thioesterase domain catalyzing Claisen cyclization in aflatoxin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 107:6246-51 (2010)
DOI:10.1073/pnas.0913531107
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.221
IUBMB Enzyme Nomenclature: 2.3.1.221
ExPASy - ENZYME nomenclature database: 2.3.1.221
BRENDA, the Enzyme Database: 2.3.1.221

KEGG   REACTION: R10239Help
Entry
R10239                      Reaction                               

Name
malonyl-CoA:hexanoate malonyltransferase (norsolorinic acid anthrone-forming)
Definition
7 Malonyl-CoA + Hexanoyl-[acp] <=> 7 CoA + Norsolorinic acid anthrone + Acyl-carrier protein + 7 CO2 + 2 H2O
Equation
7 C00083 + C05749 <=> 7 C00010 + C20453 + C00229 + 7 C00011 + 2 C00001
Reaction class
RC00004  C00010_C00083
RC00039  C00229_C05749
Enzyme
Pathway
rn00254  Aflatoxin biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K15316  noranthrone synthase [EC:2.3.1.221]
Other DBs
RHEA: 35182

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