KEGG   ORTHOLOGY: K15359Help
Entry
K15359                      KO                                     

Name
hspA
Definition
6-hydroxy-3-succinoylpyridine 3-monooxygenase [EC:1.14.13.163]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00811  Nicotine degradation, pyrrolidine pathway, nicotine => succinate semialdehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K15359  hspA; 6-hydroxy-3-succinoylpyridine 3-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.163  6-hydroxy-3-succinoylpyridine 3-monooxygenase
     K15359  hspA; 6-hydroxy-3-succinoylpyridine 3-monooxygenase
BRITE hierarchy
Other DBs
RN: R09472
GO: 0034910
Genes
PPT: PPS_0380
PPUH: B479_02405
PPUD: DW66_0394
PMON: X969_00225
PMOT: X970_00225
TaxonomyKoalaUniProt
Reference
  Authors
Tang H, Wang S, Ma L, Meng X, Deng Z, Zhang D, Ma C, Xu P
  Title
A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16.
  Journal
Appl Environ Microbiol 74:1567-74 (2008)
DOI:10.1128/AEM.02529-07
  Sequence
[ppt:PPS_0380]

KEGG   ORTHOLOGY: K18276Help
Entry
K18276                      KO                                     

Name
hspB
Definition
6-hydroxy-3-succinoylpyridine 3-monooxygenase [EC:1.14.13.163]
Pathway
ko00760  Nicotinate and nicotinamide metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00811  Nicotine degradation, pyrrolidine pathway, nicotine => succinate semialdehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09108 Metabolism of cofactors and vitamins
   00760 Nicotinate and nicotinamide metabolism
    K18276  hspB; 6-hydroxy-3-succinoylpyridine 3-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.163  6-hydroxy-3-succinoylpyridine 3-monooxygenase
     K18276  hspB; 6-hydroxy-3-succinoylpyridine 3-monooxygenase
BRITE hierarchy
Other DBs
RN: R09472
GO: 0034910
Genes
PPT: PPS_4061
PMON: X969_19895
PMOT: X970_19530
TaxonomyKoalaUniProt
Reference
  Authors
Tang H, Yao Y, Zhang D, Meng X, Wang L, Yu H, Ma L, Xu P
  Title
A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida.
  Journal
J Biol Chem 286:39179-87 (2011)
DOI:10.1074/jbc.M111.283929
  Sequence
[ppt:PPS_4061]

KEGG   ENZYME: 1.14.13.163Help
Entry
EC 1.14.13.163              Enzyme                                 

Name
6-hydroxy-3-succinoylpyridine 3-monooxygenase;
6-hydroxy-3-succinoylpyridine hydroxylase;
hspA (gene name);
hspB (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate,NADH:oxygen oxidoreductase (3-hydroxylating, succinate semialdehyde releasing)
Reaction(IUBMB)
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 = 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O [RN:R09472]
Reaction(KEGG)
Substrate
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate [CPD:C19631];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,5-dihydroxypyridine [CPD:C01059];
succinate semialdehyde [CPD:C00232];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
The enzyme catalyses a reaction in the nicotine degradation pathway of Pseudomonas species. One of the enzymes from the soil bacterium Pseudomonas putida S16 contains an FAD cofactor [2].
History
EC 1.14.13.163 created 2012
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15359  6-hydroxy-3-succinoylpyridine 3-monooxygenase
K18276  6-hydroxy-3-succinoylpyridine 3-monooxygenase
Genes
PPT: PPS_0380 PPS_4061
PPUH: B479_02405
PPUD: DW66_0394
PMON: X969_00225 X969_19895
PMOT: X970_00225 X970_19530
Taxonomy
Reference
1  [PMID:18203859]
  Authors
Tang H, Wang S, Ma L, Meng X, Deng Z, Zhang D, Ma C, Xu P
  Title
A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16.
  Journal
Appl Environ Microbiol 74:1567-74 (2008)
DOI:10.1128/AEM.02529-07
  Sequence
[ppt:PPS_0380]
Reference
2  [PMID:21949128]
  Authors
Tang H, Yao Y, Zhang D, Meng X, Wang L, Yu H, Ma L, Xu P
  Title
A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida.
  Journal
J Biol Chem 286:39179-87 (2011)
DOI:10.1074/jbc.M111.283929
  Sequence
[ppt:PPS_4061]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.163
IUBMB Enzyme Nomenclature: 1.14.13.163
ExPASy - ENZYME nomenclature database: 1.14.13.163
BRENDA, the Enzyme Database: 1.14.13.163

KEGG   REACTION: R09472Help
Entry
R09472                      Reaction                               

Name
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate,NADH:oxygen oxidoreductase (3-hydroxylating, succinate semialdehyde releasing)
Definition
6-Hydroxy-3-succinoylpyridine + 2 NADH + 2 H+ + Oxygen <=> 2,5-Dihydroxypyridine + Succinate semialdehyde + 2 NAD+ + H2O
Equation
Reaction class
RC00001  C00003_C00004
RC03005  C01059_C19631
RC03006  C00232_C19631
Enzyme
Pathway
rn00760  Nicotinate and nicotinamide metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00811  Nicotine degradation, pyrrolidine pathway, nicotine => succinate semialdehyde
Orthology
K15359  6-hydroxy-3-succinoylpyridine 3-monooxygenase [EC:1.14.13.163]
K18276  6-hydroxy-3-succinoylpyridine 3-monooxygenase [EC:1.14.13.163]
Other DBs
RHEA: 33930

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