KEGG   ORTHOLOGY: K15467Help
Entry
K15467                      KO                                     

Name
rif14
Definition
27-O-demethylrifamycin SV methyltransferase [EC:2.1.1.315]
Pathway
ko01051  Biosynthesis of ansamycins
ko01052  Type I polyketide structures
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K15467  rif14; 27-O-demethylrifamycin SV methyltransferase
   01051 Biosynthesis of ansamycins
    K15467  rif14; 27-O-demethylrifamycin SV methyltransferase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01008 Polyketide biosynthesis proteins
    K15467  rif14; 27-O-demethylrifamycin SV methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.315  27-O-demethylrifamycin SV methyltransferase
     K15467  rif14; 27-O-demethylrifamycin SV methyltransferase
Polyketide biosynthesis proteins [BR:ko01008]
 Polyketide tailoring proteins
  Methylase
   K15467  rif14; 27-O-demethylrifamycin SV methyltransferase
BRITE hierarchy
Other DBs
RN: R06998
Genes
AMD: AMED_0650
AMN: RAM_03320
AMM: AMES_0648
AMZ: B737_0649
SAQ: Sare_1271
TaxonomyKoalaUniProt
Reference
PMID:9512878
  Authors
August PR, Tang L, Yoon YJ, Ning S, Muller R, Yu TW, Taylor M, Hoffmann D, Kim CG, Zhang X, Hutchinson CR, Floss HG
  Title
Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699.
  Journal
Chem Biol 5:69-79 (1998)
DOI:10.1016/S1074-5521(98)90141-7
  Sequence
[amn:RAM_03320]
Reference
  Authors
Xu J, Mahmud T, Floss HG
  Title
Isolation and characterization of 27-O-demethylrifamycin SV methyltransferase provides new insights into the post-PKS modification steps during the biosynthesis of the antitubercular drug rifamycin B by Amycolatopsis mediterranei S699.
  Journal
Arch Biochem Biophys 411:277-88 (2003)
DOI:10.1016/S0003-9861(03)00004-3

KEGG   ENZYME: 2.1.1.315Help
Entry
EC 2.1.1.315                Enzyme                                 

Name
27-O-demethylrifamycin SV methyltransferase;
AdoMet:27-O-demethylrifamycin SV methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:27-O-demethylrifamycin-SV 27-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 27-O-demethylrifamycin SV = S-adenosyl-L-homocysteine + rifamycin SV [RN:R06998]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
27-O-demethylrifamycin SV [CPD:C14727]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
rifamycin SV [CPD:C12044]
Comment
The enzyme, characterized from the bacterium Amycolatopsis mediterranei, is involved in biosynthesis of the antitubercular drug rifamycin B.
History
EC 2.1.1.315 created 2015
Pathway
ec01051  Biosynthesis of ansamycins
ec01130  Biosynthesis of antibiotics
Orthology
K15467  27-O-demethylrifamycin SV methyltransferase
Genes
AMD: AMED_0650
AMN: RAM_03320
AMM: AMES_0648
AMZ: B737_0649
SAQ: Sare_1271
Taxonomy
Reference
1  [PMID:12623077]
  Authors
Xu J, Mahmud T, Floss HG.
  Title
Isolation and characterization of 27-O-demethylrifamycin SV methyltransferase provides new insights into the post-PKS modification steps during the biosynthesis of the antitubercular drug rifamycin B by Amycolatopsis mediterranei S699.
  Journal
Arch Biochem Biophys 411:277-88 (2003)
DOI:10.1016/S0003-9861(03)00004-3
  Sequence
[amn:RAM_03320]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.315
IUBMB Enzyme Nomenclature: 2.1.1.315
ExPASy - ENZYME nomenclature database: 2.1.1.315
BRENDA, the Enzyme Database: 2.1.1.315

KEGG   REACTION: R06998Help
Entry
R06998                      Reaction                               

Name
S-adenosyl-L-methionine:27-O-demethylrifamycin-SV 27-O-methyltransferase
Definition
S-Adenosyl-L-methionine + 27-O-Demethylrifamycin SV <=> S-Adenosyl-L-homocysteine + Rifamycin
Equation
Comment
Rif-orf14
Reaction class
RC00003  C00019_C00021
RC01746  C12044_C14727
Enzyme
Pathway
rn01051  Biosynthesis of ansamycins
rn01130  Biosynthesis of antibiotics
Orthology
K15467  27-O-demethylrifamycin SV methyltransferase [EC:2.1.1.315]
Other DBs
RHEA: 44743

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