KEGG   ORTHOLOGY: K15760
Entry
K15760                      KO                                     

Name
tmoA, tbuA1, touA
Definition
toluene monooxygenase system protein A [EC:1.14.13.236 1.14.13.-]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15760  tmoA, tbuA1, touA; toluene monooxygenase system protein A
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.236  toluene 4-monooxygenase
     K15760  tmoA, tbuA1, touA; toluene monooxygenase system protein A
    1.14.13.-  
     K15760  tmoA, tbuA1, touA; toluene monooxygenase system protein A
Other DBs
RN: R02550 R03562 R05666
Genes
PSD: DSC_04405
MSR: AU15_20735
CZA: CYCME_2098
THIS: HZT40_03325
SINI: GT972_13790
TBN: TBH_C0915
VFF: VITFI_CDS2830
RPI: Rpic_4632
RPF: Rpic12D_3556
CNC: CNE_BB1p12360(tmoA)
RME: Rmet_1316(tmoA)
CTI: pRALTA_0461(tmoA)
BCON: NL30_24535
BUG: BC1001_PA6410(tmoA)
BPX: BUPH_08274(tmoA)
BHO: D560_1531(tmoA)
BHM: D558_1522(tmoA)
HPSE: HPF_01805(tmoA1)
MPT: Mpe_A0814(tbuA1)
DAR: Daro_3820
ACIB: ACBT_0273(tmoA)
BBT: BBta_1598
AOL: S58_10670
HMC: HYPMC_2245(tmoA)
BTS: Btus_2069
SAY: TPY_0635
MSHG: MSG_02600(mphN)
MPHL: MPHLCCUG_04541(tmoA)
MHAS: MHAS_04833(tmoA)
MTER: 4434518_03550(tmoA)
NFR: ERS450000_00312(tmoA)
TRO: trd_A0919
SSO: SSO1227(tmoA) SSO1228(tmoA)
SOL: Ssol_2196
SSOA: SULA_2229
SSOL: SULB_2230
SSOF: SULC_2227
POG: Pogu_1801
AG: AAA25999(tmoA)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992

KEGG   ORTHOLOGY: K15761
Entry
K15761                      KO                                     

Name
tmoB, tbuU, touB
Definition
toluene monooxygenase system protein B [EC:1.14.13.236 1.14.13.-]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15761  tmoB, tbuU, touB; toluene monooxygenase system protein B
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.236  toluene 4-monooxygenase
     K15761  tmoB, tbuU, touB; toluene monooxygenase system protein B
    1.14.13.-  
     K15761  tmoB, tbuU, touB; toluene monooxygenase system protein B
Other DBs
RN: R02550 R03562 R05666
Genes
PSD: DSC_04400
MSR: AU15_20740
CZA: CYCME_2097
THIS: HZT40_03320
SINI: GT972_13795
TBN: TBH_C0916
VFF: VITFI_CDS2831
RPI: Rpic_4633
RPF: Rpic12D_3557
CNC: CNE_BB1p12350(tmoB)
RME: Rmet_1315(tmoB)
CTI: pRALTA_0462(tmoB)
BCON: NL30_24540
BUG: BC1001_PA6409(tmoB)
BHO: D560_1530
BHM: D558_1521
HPSE: HPF_01800(tmoB)
MPT: Mpe_A0815(tbuU) Mpe_A2540(tbuU)
DAR: Daro_3819
ACIB: ACBT_0272(tmoB)
BBT: BBta_1597
AOL: S58_10660
BTS: Btus_2068
SAY: TPY_0636
MSHG: MSG_02601(tmoB)
MPHL: MPHLCCUG_04540(tmoB)
MHAS: MHAS_04834(tmoB)
MTER: 4434518_03549(tmoB)
NFR: ERS450000_00313(tmoB)
TRO: trd_A0920
AG: AAA26000(tmoB)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992

KEGG   ORTHOLOGY: K15762
Entry
K15762                      KO                                     

Name
tmoC, tbuB, touC
Definition
toluene monooxygenase system ferredoxin subunit
Pathway
ko00623  Toluene degradation
ko00920  Sulfur metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K15762  tmoC, tbuB, touC; toluene monooxygenase system ferredoxin subunit
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15762  tmoC, tbuB, touC; toluene monooxygenase system ferredoxin subunit
Other DBs
RN: R02550 R03562 R05666 R09513
Genes
PSD: DSC_04395
MCA: MCA0225 MCA0586
METU: GNH96_03875 GNH96_15290
MMAI: sS8_1908 sS8_4250 sS8_5417 sS8_5458
CZA: CYCME_2096
THIS: HZT40_03315
HAZ: A9404_01470
SINI: GT972_13800
GBI: PG2T_00355
TBN: TBH_C0917
RPI: Rpic_4634
CNC: CNE_BB1p12340(tbcC)
RME: Rmet_1314(tmoC)
BCON: NL30_24545
BUG: BC1001_PA6408(tmoC)
PPNO: DA70_22380
BPE: BP0065
BPC: BPTD_0063
BPER: BN118_3397
BPET: B1917_0068
BPEU: Q425_680
BPA: BPP0518
BBR: BB0523
BHO: D560_1527
BHM: D558_1518
BTRM: SAMEA390648701723(ndoA)
PNA: Pnap_2045
HPSE: HPF_01795(tmoC)
MPT: Mpe_A0816(tbuB) Mpe_A0922(msmC) Mpe_A2541(tbuB)
DAR: Daro_3818
AZO: azo2601
AOA: dqs_2762
ACIB: ACBT_0271(tmoC)
BRA: BRADO3598
MSL: Msil_0930
FIL: BN1229_v1_3547(msmC)
FIY: BN1229_v1_2376(msmC)
BTS: Btus_2067
SAY: TPY_0637
MSHG: MSG_02602
MPHL: MPHLCCUG_04539(tmoC)
MHAS: MHAS_04835(tmoC)
MTER: 4434518_03548(tmoC)
NFR: ERS450000_00317(tmoC)
TRO: trd_A0921
SSO: SSO1230(tmoC)
SOL: Ssol_2198
SSOA: SULA_2231
SSOL: SULB_2232
SSOF: SULC_2229
SID: M164_0422
SII: LD85_0413
SIH: SiH_0429
SIR: SiRe_0387
PAS: Pars_0544
AG: AAA26001(tmoC)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992

KEGG   ORTHOLOGY: K15763
Entry
K15763                      KO                                     

Name
tmoD, tbuV, touD
Definition
toluene monooxygenase system protein D [EC:1.14.13.236 1.14.13.-]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15763  tmoD, tbuV, touD; toluene monooxygenase system protein D
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.236  toluene 4-monooxygenase
     K15763  tmoD, tbuV, touD; toluene monooxygenase system protein D
    1.14.13.-  
     K15763  tmoD, tbuV, touD; toluene monooxygenase system protein D
Other DBs
RN: R02550 R03562 R05666
Genes
PSD: DSC_04390
MSR: AU15_20750
CZA: CYCME_2095
SINI: GT972_13805
TBN: TBH_C0918
VFF: VITFI_CDS2833
RPI: Rpic_4635
RPF: Rpic12D_3559
CNC: CNE_BB1p12330(tmoD)
REU: Reut_B5676
RME: Rmet_1313(tmoD)
CTI: pRALTA_0465(tmoD)
BCON: NL30_24550
BUG: BC1001_PA6407(tmoD)
BHO: D560_1529
BHM: D558_1520
HPSE: HPF_01790(tmoD)
MPT: Mpe_A0817(tbuV) Mpe_A2542(tbuV)
DAR: Daro_3817
ACIB: ACBT_0270(tmoD)
BBT: BBta_1595
AOL: S58_10640
HMC: HYPMC_2242(tmoD)
BTS: Btus_2066
SAY: TPY_0638
MSHG: MSG_02603(tmoD)
MPHL: MPHLCCUG_04538(tmoD)
MHAS: MHAS_04836(tmoD)
MTER: 4434518_03547(tmoD)
NFR: ERS450000_00314(tmoD)
TRO: trd_A0922
SSO: SSO1231(tmoD)
SOL: Ssol_2199
SSOA: SULA_2232
SSOL: SULB_2233
SSOF: SULC_2230
SID: M164_0423
SII: LD85_0414
SIH: SiH_0430
SIR: SiRe_0388
PAS: Pars_0543
AG: AAA26002(tmoD)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992

KEGG   ORTHOLOGY: K15764
Entry
K15764                      KO                                     

Name
tmoE, tbuA2, touE
Definition
toluene monooxygenase system protein E [EC:1.14.13.236 1.14.13.-]
Pathway
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15764  tmoE, tbuA2, touE; toluene monooxygenase system protein E
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.236  toluene 4-monooxygenase
     K15764  tmoE, tbuA2, touE; toluene monooxygenase system protein E
    1.14.13.-  
     K15764  tmoE, tbuA2, touE; toluene monooxygenase system protein E
Other DBs
RN: R02550 R03562 R05666
Genes
PSD: DSC_04385
MSR: AU15_20755
CZA: CYCME_2094
THIS: HZT40_03305
SINI: GT972_13810
TBN: TBH_C0919
VFF: VITFI_CDS2834
RPI: Rpic_4636
RPF: Rpic12D_3560
CNC: CNE_BB1p12320(tmoE)
RME: Rmet_1312(tmoE)
CTI: pRALTA_0466(tmoE)
BCON: NL30_24555
BUG: BC1001_PA6406(tmoE)
BHO: D560_1528(touE)
BHM: D558_1519(touE)
HPSE: HPF_01785(tmoE)
MPT: Mpe_A0818(tbuA2) Mpe_A2543(tbuA2)
DAR: Daro_3816
ACIB: ACBT_0269(tmoE)
BBT: BBta_1594
AOL: S58_10630
HMC: HYPMC_2241(tmoE)
BTS: Btus_2065
SAY: TPY_0639
MSHG: MSG_02604
MPHL: MPHLCCUG_04537(tmoE)
MHAS: MHAS_04837(tmoE)
MTER: 4434518_03546(tmoE)
NFR: ERS450000_00315(tmoE)
TRO: trd_A0923
SSO: SSO1233(tmoE)
SOL: Ssol_2200
SSOA: SULA_2233
SSOL: SULB_2234
SSOF: SULC_2231
SID: M164_0424
SII: LD85_0415
SIH: SiH_0431
SIR: SiRe_0389
SIC: SiL_0415
PAS: Pars_0542
AG: AAA26003(tmoE)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
  Sequence
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992

KEGG   ORTHOLOGY: K15765
Entry
K15765                      KO                                     

Name
tmoF, tbuC, touF
Definition
toluene monooxygenase electron transfer component [EC:1.18.1.3]
Pathway
ko00623  Toluene degradation
ko00920  Sulfur metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K15765  tmoF, tbuC, touF; toluene monooxygenase electron transfer component
Other DBs
RN: R02550 R03562 R05666 R09513
Genes
PSD: DSC_04380
PPSE: BN5_3604
MBS: MRBBS_1308(tmoF)
MSR: AU15_20760
CYQ: Q91_2220
CZA: CYCME_2538
CYY: CPC19_01700
THIS: HZT40_03300
APAC: S7S_13335
SINI: GT972_13815
TBN: TBH_C0920
RPI: Rpic_4637
CNC: CNE_BB1p12310(tmoF)
RME: Rmet_1311
BCT: GEM_5810
BCON: NL30_24560
BUG: BC1001_PA6405(tmoF)
PPNO: DA70_22375
BPE: BP0066
BPC: BPTD_0064
BPER: BN118_3398
BPET: B1917_0069
BPEU: Q425_690
BPA: BPP0517
BBR: BB0522
BTRM: SAMEA390648701722(dmpP_1)
PUT: PT7_2459
LIM: L103DPR2_00189(carAd)
HPSE: HPF_01780(tmoF)
MPT: Mpe_A0819(tbuC) Mpe_A0921(msmD)
DAR: Daro_3815
BBT: BBta_1593
AOL: S58_10620
OCA: OCAR_6789
FIL: BN1229_v1_3546(msmD)
FIY: BN1229_v1_2377(msmD)
HDI: HDIA_4247(carAd)
SGI: SGRAN_2803(thnA4)
BTS: Btus_2064
NFR: ERS450000_00316(tmoF)
TRO: trd_A0924
AG: AAA26004(tmoF)
 » show all
Reference
PMID:1885512
  Authors
Yen KM, Karl MR, Blatt LM, Simon MJ, Winter RB, Fausset PR, Lu HS, Harcourt AA, Chen KK
  Title
Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 173:5315-27 (1991)
DOI:10.1128/JB.173.17.5315-5327.1991
Reference
PMID:1429451
  Authors
Yen KM, Karl MR
  Title
Identification of a new gene, tmoF, in the Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase.
  Journal
J Bacteriol 174:7253-61 (1992)
DOI:10.1128/JB.174.22.7253-7261.1992
  Sequence

KEGG   ORTHOLOGY: K15757
Entry
K15757                      KO                                     

Name
xylM
Definition
toluene methyl-monooxygenase [EC:1.14.15.26]
Pathway
ko00622  Xylene degradation
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00537  Xylene degradation, xylene => methylbenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15757  xylM; toluene methyl-monooxygenase
   00622 Xylene degradation
    K15757  xylM; toluene methyl-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.15  With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.15.26  toluene methyl-monooxygenase
     K15757  xylM; toluene methyl-monooxygenase
Other DBs
RN: R02550 R05288 R05442 R05443
GO: 0018639
Genes
PSD: DSC_14810
NAR: Saro_3845
NPP: PP1Y_AT31329
SPHY: CHN51_07015
AG: AAA26026(xylM)
Reference
PMID:1999388
  Authors
Suzuki M, Hayakawa T, Shaw JP, Rekik M, Harayama S
  Title
Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system.
  Journal
J Bacteriol 173:1690-5 (1991)
DOI:10.1128/JB.173.5.1690-1695.1991
  Sequence

KEGG   ORTHOLOGY: K15758
Entry
K15758                      KO                                     

Name
xylA
Definition
toluene methyl-monooxygenase electron transfer component [EC:1.18.1.3]
Pathway
ko00622  Xylene degradation
ko00623  Toluene degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00537  Xylene degradation, xylene => methylbenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00623 Toluene degradation
    K15758  xylA; toluene methyl-monooxygenase electron transfer component
   00622 Xylene degradation
    K15758  xylA; toluene methyl-monooxygenase electron transfer component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K15758  xylA; toluene methyl-monooxygenase electron transfer component
Other DBs
RN: R02550 R05288 R05442 R05443
GO: 0018639
Genes
PSD: DSC_14805
NAR: Saro_3846
NPP: PP1Y_AT15752 PP1Y_AT31343
SPHY: CHN51_07010
AG: AAA26027(xylA)
Reference
PMID:1999388
  Authors
Suzuki M, Hayakawa T, Shaw JP, Rekik M, Harayama S
  Title
Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system.
  Journal
J Bacteriol 173:1690-5 (1991)
DOI:10.1128/JB.173.5.1690-1695.1991
  Sequence

KEGG   ENZYME: 1.14.15.26
Entry
EC 1.14.15.26               Enzyme                                 

Name
toluene methyl-monooxygenase;
xylM (gene names);
ntnM (gene names)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
methylbenzene,ferredoxin:oxygen oxidoreductase (methyl-hydroxylating)
Reaction(IUBMB)
(1) toluene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = benzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R02550];
(2) p-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = 4-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R05288];
(3) m-xylene + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ = 3-methylbenzyl alcohol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R05443]
Reaction(KEGG)
R02550 R05288 R05443;
(other) R05442
Substrate
toluene [CPD:C01455];
O2 [CPD:C00007];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
p-xylene [CPD:C06756];
m-xylene [CPD:C07208]
Product
benzyl alcohol [CPD:C00556];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
4-methylbenzyl alcohol [CPD:C06757];
3-methylbenzyl alcohol [CPD:C07216]
Comment
The enzyme, characterized from several Pseudomonas strains, catalyses the first step in the degradation of toluenes and xylenes. It has a broad substrate specificity and is also active with substituted compounds, such as chlorotoluenes. The electrons are provided by a reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase) that transfers electrons from NADH via FAD and an [2Fe-2S] cluster. The enzyme can also act on its products, producing gem-diols that spontaneously dehydrate to form aldehydes.
History
EC 1.14.15.26 created 2018
Pathway
ec00622  Xylene degradation
ec00623  Toluene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15757  toluene methyl-monooxygenase
Genes
PSD: DSC_14810
NAR: Saro_3845
NPP: PP1Y_AT31329
SPHY: CHN51_07015
Reference
1  [PMID:1999388]
  Authors
Suzuki M, Hayakawa T, Shaw JP, Rekik M, Harayama S
  Title
Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system.
  Journal
J Bacteriol 173:1690-5 (1991)
DOI:10.1128/JB.173.5.1690-1695.1991
Reference
2  [PMID:1327782]
  Authors
Shaw JP, Harayama S
  Title
Purification and characterisation of the NADH:acceptor reductase component of xylene monooxygenase encoded by the TOL plasmid pWW0 of Pseudomonas putida mt-2.
  Journal
Eur J Biochem 209:51-61 (1992)
DOI:10.1111/j.1432-1033.1992.tb17260.x
Reference
3  [PMID:1526468]
  Authors
Brinkmann U, Reineke W
  Title
Degradation of chlorotoluenes by in vivo constructed hybrid strains: problems of  enzyme specificity, induction and prevention of meta-pathway.
  Journal
FEMS Microbiol Lett 75:81-7 (1992)
DOI:10.1016/0378-1097(92)90460-6
Reference
4  [PMID:9555884]
  Authors
James KD, Williams PA
  Title
ntn genes determining the early steps in the divergent catabolism of 4-nitrotoluene and toluene in Pseudomonas sp. strain TW3.
  Journal
J Bacteriol 180:2043-9 (1998)
DOI:10.1128/JB.180.8.2043-2049.1998
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.26
IUBMB Enzyme Nomenclature: 1.14.15.26
ExPASy - ENZYME nomenclature database: 1.14.15.26
BRENDA, the Enzyme Database: 1.14.15.26

KEGG   REACTION: R02550
Entry
R02550                      Reaction                               

Name
toluene, ferredoxin:oxygen oxidoreductase (methyl-hydroxylating);
toluene/o-xylene monooxygenase
Definition
Benzyl alcohol + 2 Oxidized ferredoxin + H2O <=> Toluene + Oxygen + 2 Reduced ferredoxin + 2 H+
Equation
Comment
Oxidized ferredoxin is re-reduced by NADH (see R05875)
Reaction class
RC00269  C00556_C01455
Enzyme
1.14.13.-       1.14.15.26
Pathway
rn00623  Toluene degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00538  Toluene degradation, toluene => benzoate
Orthology
K15757  toluene methyl-monooxygenase [EC:1.14.15.26]
K15758  toluene methyl-monooxygenase electron transfer component [EC:1.18.1.3]
K15760  toluene monooxygenase system protein A [EC:1.14.13.236 1.14.13.-]
K15761  toluene monooxygenase system protein B [EC:1.14.13.236 1.14.13.-]
K15762  toluene monooxygenase system ferredoxin subunit
K15763  toluene monooxygenase system protein D [EC:1.14.13.236 1.14.13.-]
K15764  toluene monooxygenase system protein E [EC:1.14.13.236 1.14.13.-]
K15765  toluene monooxygenase electron transfer component [EC:1.18.1.3]
Other DBs
RHEA: 51595

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