KEGG   ORTHOLOGY: K15877
Entry
K15877                      KO                                     

Name
CYP55
Definition
fungal nitric oxide reductase [EC:1.7.1.14]
Pathway
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00910 Nitrogen metabolism
    K15877  CYP55; fungal nitric oxide reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.7  Acting on other nitrogenous compounds as donors
   1.7.1  With NAD+ or NADP+ as acceptor
    1.7.1.14  nitric oxide reductase [NAD(P)+, nitrous oxide-forming]
     K15877  CYP55; fungal nitric oxide reductase
Other DBs
RN: R02492 R09446 R09808 R09809
GO: 0016966
Genes
CRE: CHLREDRAFT_196664(CYP55B1)
CVR: CHLNCDRAFT_51513
NCR: NCU06137
NTE: NEUTE1DRAFT117740(NEUTE1DRAFT_117740)
SMP: SMAC_06378
PAN: PODANSg1411
TTT: THITE_2117644
FGR: FGSG_11585
FVR: FVEG_10773
FOX: FOXG_12350
MBE: MBM_04680
ANG: ANI_1_2092064(An07g08930)
ABE: ARB_00546
TVE: TRV_00378
TASA: A1Q1_03733
AG: AAA33337(CYP55A1)
 » show all
Reference
  Authors
Zhang L, Shoun H
  Title
Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor.
  Journal
Methods Enzymol 437:117-33 (2008)
DOI:10.1016/S0076-6879(07)37007-9

KEGG   ENZYME: 1.7.1.14
Entry
EC 1.7.1.14                 Enzyme                                 

Name
nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
Sysname
nitrous oxide:NAD(P) oxidoreductase
Reaction(IUBMB)
N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:R02492 R09446]
Reaction(KEGG)
R02492 R09446;
(other) R09808 R09809
Substrate
N2O [CPD:C00887];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
NO [CPD:C00533];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.
History
EC 1.7.1.14 created 2011
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase
Genes
CRE: CHLREDRAFT_196664(CYP55B1)
CVR: CHLNCDRAFT_51513
NCR: NCU06137
NTE: NEUTE1DRAFT117740(NEUTE1DRAFT_117740)
SMP: SMAC_06378
PAN: PODANSg1411
TTT: THITE_2117644
FGR: FGSG_11585
FVR: FVEG_10773
FOX: FOXG_12350
MBE: MBM_04680
ANG: ANI_1_2092064(An07g08930)
ABE: ARB_00546
TVE: TRV_00378
TASA: A1Q1_03733
 » show all
Reference
1  [PMID:2040619]
  Authors
Shoun H, Tanimoto T
  Title
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
  Journal
J Biol Chem 266:11078-82 (1991)
  Sequence
Reference
2  [PMID:7829493]
  Authors
Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H
  Title
Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.
  Journal
J Biol Chem 270:1617-23 (1995)
DOI:10.1074/jbc.270.4.1617
  Sequence
Reference
3  [PMID:12105197]
  Authors
Zhang L, Kudo T, Takaya N, Shoun H
  Title
The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.
  Journal
J Biol Chem 277:33842-7 (2002)
DOI:10.1074/jbc.M203923200
  Sequence
Reference
4  [PMID:15313618]
  Authors
Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H
  Title
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.
  Journal
J Mol Biol 342:207-17 (2004)
DOI:10.1016/j.jmb.2004.07.009
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.14
IUBMB Enzyme Nomenclature: 1.7.1.14
ExPASy - ENZYME nomenclature database: 1.7.1.14
BRENDA, the Enzyme Database: 1.7.1.14

KEGG   REACTION: R02492
Entry
R02492                      Reaction                               

Name
nitrous-oxide:NAD+ oxidoreductase
Definition
2 Nitric oxide + NADH + H+ <=> Nitrous oxide + NAD+ + H2O
Equation
Comment
NADPH (see R09446)
P450nor
Reaction class
RC00001  C00003_C00004
RC02794  C00533_C00887
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase [EC:1.7.1.14]
Other DBs
RHEA: 29610

KEGG   REACTION: R09446
Entry
R09446                      Reaction                               

Name
nitrous-oxide:NADP+ oxidoreductase
Definition
2 Nitric oxide + NADPH + H+ <=> Nitrous oxide + NADP+ + H2O
Equation
Comment
NADH (see R02492)
P450nor
Reaction class
RC00001  C00005_C00006
RC02794  C00533_C00887
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase [EC:1.7.1.14]
Other DBs
RHEA: 29614

KEGG   REACTION: R09808
Entry
R09808                      Reaction                               

Definition
Nitric oxide + Azide <=> Nitrous oxide + Nitrogen
Equation
Comment
incomplete reaction
Reaction class
RC02794  C00533_C00887
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase [EC:1.7.1.14]

KEGG   REACTION: R09809
Entry
R09809                      Reaction                               

Definition
Nitric oxide + Ammonia <=> Nitrous oxide + H2O
Equation
Comment
incomplete reaction
Reaction class
RC02794  C00533_C00887
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase [EC:1.7.1.14]

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