KEGG   ORTHOLOGY: K15886Help
Entry
K15886                      KO                                     

Name
tcmN
Definition
multifunctional cyclase / dehydratase / O-methyltransferase [EC:2.3.1.235]
Pathway
ko01056  Biosynthesis of type II polyketide backbone
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00778  Type II polyketide backbone biosynthesis, acyl-CoA + malonyl-CoA => polyketide
M00783  Tetracenomycin C/8-demethyltetracenomycin C biosynthesis, tetracenomycin F2 => tetracenomycin C/8-demethyltetracenomycin C
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01056 Biosynthesis of type II polyketide backbone
    K15886  tcmN; multifunctional cyclase / dehydratase / O-methyltransferase
   01057 Biosynthesis of type II polyketide products
    K15886  tcmN; multifunctional cyclase / dehydratase / O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.1  Transferring groups other than aminoacyl groups
    2.3.1.235  tetracenomycin F2 synthase
     K15886  tcmN; multifunctional cyclase / dehydratase / O-methyltransferase
BRITE hierarchy
Other DBs
RN: R06701 R09269 R10960
Genes
SGU: SGLAU_26370(tcmN)
TaxonomyKoalaUniProt
Reference
PMID:1548230
  Authors
Summers RG, Wendt-Pienkowski E, Motamedi H, Hutchinson CR
  Title
Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase.
  Journal
J Bacteriol 174:1810-20 (1992)
DOI:10.1128/JB.174.6.1810-1820.1992
  Sequence
Reference
  Authors
Ames BD, Korman TP, Zhang W, Smith P, Vu T, Tang Y, Tsai SC
  Title
Crystal structure and functional analysis of tetracenomycin ARO/CYC: implications for cyclization specificity of aromatic polyketides.
  Journal
Proc Natl Acad Sci U S A 105:5349-54 (2008)
DOI:10.1073/pnas.0709223105

KEGG   ENZYME: 2.3.1.235Help
Entry
EC 2.3.1.235                Enzyme                                 

Name
tetracenomycin F2 synthase;
TCM PKS
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
malonyl-CoA:acetate malonyltransferase (tetracenomycin F2 forming)
Reaction(IUBMB)
10 malonyl-CoA = tetracenomycin F2 + 10 CoA + 10 CO2 + 2 H2O [RN:R10960]
Reaction(KEGG)
R10960;
(other) R06643 R09269
Show
Substrate
malonyl-CoA [CPD:C00083]
Product
tetracenomycin F2 [CPD:C12366];
CoA [CPD:C00010];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
A multi-domain polyketide synthase involved in the synthesis of tetracenomycin in the bacterium Streptomyces glaucescens. It involves a ketosynthase complex (TcmKL), an acyl carrier protein (TcmM), a malonyl CoA:ACP acyltransferase (MAT), and a cyclase (TcmN). A malonyl-CoA molecule is initially bound to the acyl carrier protein and decarboxylated to form an acetyl starter unit. Additional two-carbon units are added from nine more malonyl-CoA molecules.
History
EC 2.3.1.235 created 2014
Pathway
ec01056  Biosynthesis of type II polyketide backbone
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K05551  minimal PKS ketosynthase (KS/KS alpha)
K05552  minimal PKS chain-length factor (CLF/KS beta)
K15886  multifunctional cyclase / dehydratase / O-methyltransferase
Genes
MBD: MEBOL_003040 MEBOL_003041
ABF: AMK58_25510
SPAE: E2C16_10950
MSAO: MYCSP_12695 MYCSP_12700
MSAL: DSM43276_02542 DSM43276_02543
NBR: O3I_007445 O3I_007450 O3I_015110 O3I_015115
NNO: NONO_c10800 NONO_c10810
NTP: CRH09_08670 CRH09_08675
NOZ: DMB37_03465 DMB37_03470
SCO: SCO5087(actIORF1) SCO5088(actIORF2)
SMA: SAVERM_2375(pks9-3) SAVERM_2376(pks9-4)
STRP: F750_1305(whiE-KS) F750_1306(whiE-CLF)
STRM: M444_36340
SALJ: SMD11_0675(actI1) SMD11_0676(actI2) SMD11_1144(actI2) SMD11_6044(actI1) SMD11_6045(actI2)
AMD: AMED_5434(fabF) AMED_5435(fabF)
AMM: AMES_5369(fabF) AMES_5370(fabF)
AMZ: B737_5369(fabF) B737_5370(fabF)
 » show all
Taxonomy
Reference
1  [PMID:9609708]
  Authors
Bao W, Wendt-Pienkowski E, Hutchinson CR
  Title
Reconstitution of the iterative type II polyketide synthase for tetracenomycin F2 biosynthesis.
  Journal
Biochemistry 37:8132-8 (1998)
DOI:10.1021/bi980466i
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.235
IUBMB Enzyme Nomenclature: 2.3.1.235
ExPASy - ENZYME nomenclature database: 2.3.1.235
BRENDA, the Enzyme Database: 2.3.1.235

KEGG   REACTION: R06701Help
Entry
R06701                      Reaction                               

Definition
Tetracenomycin D3 <=> Tetracenomycin B3
Equation
Comment
Multifunctional cyclase-dehydratase-3-O-methyl transferase, tcmN
Reaction class
RC00392  C12368_C12369
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00783  Tetracenomycin C/8-demethyltetracenomycin C biosynthesis, tetracenomycin F2 => tetracenomycin C/8-demethyltetracenomycin C
Orthology
K15886  multifunctional cyclase / dehydratase / O-methyltransferase [EC:2.3.1.235]

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