KEGG   ORTHOLOGY: K15907Help
Entry
K15907                      KO                                     

Name
ptlI, CYP183A
Definition
pentalenene oxygenase [EC:1.14.15.32]
Pathway
ko00909  Sesquiterpenoid and triterpenoid biosynthesis
ko00999  Biosynthesis of various secondary metabolites - part 1
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K15907  ptlI, CYP183A; pentalenene oxygenase
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various secondary metabolites - part 1
    K15907  ptlI, CYP183A; pentalenene oxygenase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K15907  ptlI, CYP183A; pentalenene oxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.15  With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.15.32  pentalenene oxygenase
     K15907  ptlI, CYP183A; pentalenene oxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, bacteria type
  CYP183 family
   K15907  ptlI, CYP183A; pentalenene oxygenase
BRITE hierarchy
Other DBs
RN: R09814
COG: COG2124
Genes
SMA: SAVERM_2999(ptlI)
SCT: SCAT_p0090(ptlI)
SCY: SCATT_p16540
SBH: SBI_09680(cyp48)
SDV: BN159_1442(ptlI)
SCI: B446_29700
SCW: TU94_27410
SLE: sle_13150(sle_13150)
SCLF: BB341_00225
SSIA: A7J05_09235
SALF: SMD44_00690(ptlI)
SALJ: SMD11_0076(ptlI)
KSK: KSE_03600
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Quaderer R, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:13036-7 (2006)
DOI:10.1021/ja0639214
  Sequence

KEGG   ENZYME: 1.14.15.32Help
Entry
EC 1.14.15.32               Enzyme                                 

Name
pentalenene oxygenase;
PtlI
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
pentalenene,reduced ferredoxin:oxygen 13-oxidoreductase
Reaction(IUBMB)
pentalenene + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = pentalen-13-al + 4 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction) [RN:R09814];
(1a) pentalenene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-ol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R09812];
(1b) pentalen-13-ol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-al + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R09813]
Reaction(KEGG)
Substrate
pentalenene [CPD:C01841];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007];
pentalen-13-ol [CPD:C19938]
Product
pentalen-13-al [CPD:C19939];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
pentalen-13-ol [CPD:C19938]
Comment
A cytochrome P-450 (heme-thiolate) protein found in the bacterium Streptomyces avermitilis. The enzyme is involved in the biosynthesis of pentalenolactone and related antibiotics.
History
EC 1.14.15.32 created 2011 as EC 1.14.13.133, transferred 2018 to EC 1.14.15.32
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K15907  pentalenene oxygenase
Genes
SMA: SAVERM_2999(ptlI)
SCT: SCAT_p0090(ptlI)
SCY: SCATT_p16540
SBH: SBI_09680(cyp48)
SDV: BN159_1442(ptlI)
SCI: B446_29700
SCW: TU94_27410
SLE: sle_13150(sle_13150)
SCLF: BB341_00225
SSIA: A7J05_09235
SALF: SMD44_00690(ptlI)
SALJ: SMD11_0076(ptlI)
KSK: KSE_03600
 » show all
Taxonomy
Reference
1  [PMID:17017767]
  Authors
Quaderer R, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:13036-7 (2006)
DOI:10.1021/ja0639214
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.32
IUBMB Enzyme Nomenclature: 1.14.15.32
ExPASy - ENZYME nomenclature database: 1.14.15.32
BRENDA, the Enzyme Database: 1.14.15.32

KEGG   REACTION: R09814Help
Entry
R09814                      Reaction                               

Name
pentalenene,reduced ferredoxin:oxygen 13-oxidoreductase
Definition
Pentalenene + 4 Reduced ferredoxin + 4 H+ + 2 Oxygen <=> Pentalen-13-al + 4 Oxidized ferredoxin + 3 H2O
Equation
C01841 + 4 C00138 + 4 C00080 + 2 C00007 <=> C19939 + 4 C00139 + 3 C00001
Comment
two-step reaction (see R09812+R09813)
Reaction class
RC02663  C01841_C19939
Enzyme
Pathway
rn00909  Sesquiterpenoid and triterpenoid biosynthesis
rn00999  Biosynthesis of various secondary metabolites - part 1
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Orthology
K15907  pentalenene oxygenase [EC:1.14.15.32]
Other DBs
RHEA: 31702

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