KEGG   ORTHOLOGY: K15941Help
Entry
K15941                      KO                                     

Name
snoaB, dnrG, aknX
Definition
deoxynogalonate / 12-deoxyaklanonic acid monooxygenase [EC:1.13.12.22 1.13.12.-]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15941  snoaB, dnrG, aknX; deoxynogalonate / 12-deoxyaklanonic acid monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.13  Acting on single donors with incorporation of molecular oxygen (oxygenases)
   1.13.12  With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
    1.13.12.22  deoxynogalonate monooxygenase
     K15941  snoaB, dnrG, aknX; deoxynogalonate / 12-deoxyaklanonic acid monooxygenase
    1.13.12.-  
     K15941  snoaB, dnrG, aknX; deoxynogalonate / 12-deoxyaklanonic acid monooxygenase
BRITE hierarchy
Other DBs
RN: R06679 R09327
Genes
MAU: Micau_3995
MIL: ML5_4426
AG: AAA65205(dnrG) AAF70105(aknX) CAA12015(snoaB)
TaxonomyKoalaUniProt
Reference
PMID:8668120
  Authors
Ylihonko K, Tuikkanen J, Jussila S, Cong L, Mantsala P
  Title
A gene cluster involved in nogalamycin biosynthesis from Streptomyces nogalater: sequence analysis and complementation of early-block mutations in the anthracycline pathway.
  Journal
Mol Gen Genet 251:113-20 (1996)
DOI:10.1007/BF02172908
  Sequence
Reference
  Authors
Grocholski T, Koskiniemi H, Lindqvist Y, Mantsala P, Niemi J, Schneider G
  Title
Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism.
  Journal
Biochemistry 49:934-44 (2010)
DOI:10.1021/bi901985b
Reference
  Authors
Chung JY, Fujii I, Harada S, Sankawa U, Ebizuka Y
  Title
Expression, purification, and characterization of AknX anthrone oxygenase, which is involved in aklavinone biosynthesis in Streptomyces galilaeus.
  Journal
J Bacteriol 184:6115-22 (2002)
DOI:10.1128/JB.184.22.6115-6122.2002
  Sequence

KEGG   ENZYME: 1.13.12.22Help
Entry
EC 1.13.12.22               Enzyme                                 

Name
deoxynogalonate monooxygenase;
SnoaB (gene name);
12-deoxynogalonic acid oxidoreductase;
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate oxidase;
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate monooxygenase;
deoxynogalonate oxidoreductase
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of one atom of oxygen (internal monooxygenases or internal mixed-function oxidases)
BRITE hierarchy
Sysname
deoxynogalonate:oxygen oxidoreductase
Reaction(IUBMB)
deoxynogalonate + O2 = nogalonate + H2O [RN:R09327]
Reaction(KEGG)
Substrate
deoxynogalonate [CPD:C18629];
O2 [CPD:C00007]
Product
nogalonate [CPD:C12416];
H2O [CPD:C00001]
Comment
The enzyme, characterized from the bacterium Streptomyces nogalater, is involved in the biosynthesis of the aromatic polyketide nogalamycin.
History
EC 1.13.12.22 created 2015
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K15941  deoxynogalonate / 12-deoxyaklanonic acid monooxygenase
Genes
MAU: Micau_3995
MIL: ML5_4426
Taxonomy
Reference
1  [PMID:19255477]
  Authors
Koskiniemi H, Grocholski T, Schneider G, Niemi J.
  Title
Expression, purification and crystallization of the cofactor-independent monooxygenase SnoaB from the nogalamycin biosynthetic pathway.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:256-9 (2009)
DOI:10.1107/S1744309109001389
  Sequence
Reference
2  [PMID:20052967]
  Authors
Grocholski T, Koskiniemi H, Lindqvist Y, Mantsala P, Niemi J, Schneider G.
  Title
Crystal structure of the cofactor-independent monooxygenase SnoaB from Streptomyces nogalater: implications for the reaction mechanism.
  Journal
Biochemistry 49:934-44 (2010)
DOI:10.1021/bi901985b
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.12.22
IUBMB Enzyme Nomenclature: 1.13.12.22
ExPASy - ENZYME nomenclature database: 1.13.12.22
BRENDA, the Enzyme Database: 1.13.12.22

KEGG   REACTION: R09327Help
Entry
R09327                      Reaction                               

Name
deoxynogalonate:oxygen oxidoreductase
Definition
Deoxynogalonate + Oxygen <=> Nogalonate + H2O
Equation
Comment
cofactor-independent monooxygenase, snoaB
Reaction class
RC01658  C12416_C18629
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Orthology
K15941  deoxynogalonate / 12-deoxyaklanonic acid monooxygenase [EC:1.13.12.22 1.13.12.-]
Other DBs
RHEA: 45059

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