KEGG   ORTHOLOGY: K15944
Entry
K15944                      KO                                     

Name
snoaF, dnrE, dauE, aknU
Definition
nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.362  aklaviketone reductase
     K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
    1.1.1.-  
     K15944  snoaF, dnrE, dauE, aknU; nogalaviketone/aklaviketone reductase
Other DBs
RN: R06677 R06682 R09329
Genes
MAU: Micau_4000
MIL: ML5_4421
AG: AAA65202(dnrE) AAB08021(dauE) AAF01806(snoaF) AAF73457(aknU)
Reference
  Authors
Torkkell S, Kunnari T, Palmu K, Mantsala P, Hakala J, Ylihonko K
  Title
The entire nogalamycin biosynthetic gene cluster of Streptomyces nogalater: characterization of a 20-kb DNA region and generation of hybrid structures.
  Journal
Mol Genet Genomics 266:276-88 (2001)
DOI:10.1007/s004380100554
  Sequence
Reference
PMID:8655529
  Authors
Dickens ML, Ye J, Strohl WR
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
Reference
  Authors
Raty K, Kunnari T, Hakala J, Mantsala P, Ylihonko K
  Title
A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin.
  Journal
Mol Gen Genet 264:164-72 (2000)
DOI:10.1007/s004380000306
  Sequence

KEGG   ENZYME: 1.1.1.362
Entry
EC 1.1.1.362                Enzyme                                 

Name
aklaviketone reductase;
dauE (gene name);
aknU (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
aklavinone:NADP+ oxidoreductase
Reaction(IUBMB)
aklavinone + NADP+ = aklaviketone + NADPH + H+ [RN:R06682]
Reaction(KEGG)
R06682;
(other) R06677
Substrate
aklavinone [CPD:C12424];
NADP+ [CPD:C00006]
Product
aklaviketone [CPD:C12423];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme is involved in the synthesis of the aklavinone aglycone, a common precursor for several anthracycline antibiotics including aclacinomycins, daunorubicin and doxorubicin. The enzyme from the Gram-negative bacterium Streptomyces sp. C5 produces daunomycin.
History
EC 1.1.1.362 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15944  nogalaviketone/aklaviketone reductase
Genes
MAU: Micau_4000
MIL: ML5_4421
Reference
1  [PMID:8655529]
  Authors
Dickens ML, Ye J, Strohl WR.
  Title
Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5.
  Journal
J Bacteriol 178:3384-8 (1996)
DOI:10.1128/JB.178.11.3384-3388.1996
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.362
IUBMB Enzyme Nomenclature: 1.1.1.362
ExPASy - ENZYME nomenclature database: 1.1.1.362
BRENDA, the Enzyme Database: 1.1.1.362

KEGG   REACTION: R06682
Entry
R06682                      Reaction                               

Name
aklavinone:NADP+ 7-oxidoreductase
Definition
Aklaviketone + NADPH + H+ <=> Aklavinone + NADP+
Equation
Reaction class
RC00001  C00005_C00006
RC00234  C12423_C12424
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00781  Nogalavinone/aklavinone biosynthesis, deoxynogalonate/deoxyaklanonate => nogalavinone/aklavinone
Orthology
K15944  nogalaviketone/aklaviketone reductase [EC:1.1.1.- 1.1.1.362]
Other DBs
RHEA: 37414

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