KEGG   ORTHOLOGY: K15948Help
Entry
K15948                      KO                                     

Name
aknK
Definition
aclacinomycin-T 2-deoxy-L-fucose transferase [EC:2.4.1.327]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15948  aknK; aclacinomycin-T 2-deoxy-L-fucose transferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.327  aclacinomycin-T 2-deoxy-L-fucose transferase
     K15948  aknK; aclacinomycin-T 2-deoxy-L-fucose transferase
BRITE hierarchy
Other DBs
RN: R09332
COG: COG1819
CAZy: GT1
Genes
AG: AAF70102(aknK)
TaxonomyKoalaUniProt
Reference
  Authors
Lu W, Leimkuhler C, Oberthur M, Kahne D, Walsh CT
  Title
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A.
  Journal
Biochemistry 43:4548-58 (2004)
DOI:10.1021/bi035945i
  Sequence
Reference
  Authors
Leimkuhler C, Fridman M, Lupoli T, Walker S, Walsh CT, Kahne D
  Title
Characterization of rhodosaminyl transfer by the AknS/AknT glycosylation complex and its use in reconstituting the biosynthetic pathway of aclacinomycin A.
  Journal
J Am Chem Soc 129:10546-50 (2007)
DOI:10.1021/ja072909o

KEGG   ENZYME: 2.4.1.327Help
Entry
EC 2.4.1.327                Enzyme                                 

Name
aclacinomycin-T 2-deoxy-L-fucose transferase;
AknK
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dTDP-2-deoxy-beta-L-fucose:7-(alpha-L-rhodosaminyl)aklavinone 2-deoxy-alpha-L-fucosyltransferase
Reaction(IUBMB)
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T = dTDP + aclacinomycin S [RN:R09332]
Reaction(KEGG)
Substrate
dTDP-2-deoxy-beta-L-fucose [CPD:C18636];
aclacinomycin T [CPD:C18634]
Product
dTDP [CPD:C00363];
aclacinomycin S [CPD:C18635]
Comment
The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
History
EC 2.4.1.327 created 2014
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01130  Biosynthesis of antibiotics
Orthology
K15948  aclacinomycin-T 2-deoxy-L-fucose transferase
Reference
1  [PMID:15078101]
  Authors
Lu W, Leimkuhler C, Oberthur M, Kahne D, Walsh CT.
  Title
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A.
  Journal
Biochemistry 43:4548-58 (2004)
DOI:10.1021/bi035945i
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.327
IUBMB Enzyme Nomenclature: 2.4.1.327
ExPASy - ENZYME nomenclature database: 2.4.1.327
BRENDA, the Enzyme Database: 2.4.1.327

KEGG   REACTION: R09332Help
Entry
R09332                      Reaction                               

Name
dTDP-2-deoxy-beta-L-fucose:aclacinomycin-T 2-deoxy-alpha-L-fucosyltransferase
Definition
Aclacinomycin T + dTDP-2-deoxy-beta-L-fucose <=> Aclacinomycin S + dTDP
Equation
Reaction class
RC00005  C00363_C18636
RC00049  C18634_C18635
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01130  Biosynthesis of antibiotics
Orthology
K15948  aclacinomycin-T 2-deoxy-L-fucose transferase [EC:2.4.1.327]
Other DBs
RHEA: 41571

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