KEGG   ORTHOLOGY: K15949Help
Entry
K15949                      KO                                     

Name
aknOx
Definition
aclacinomycin-N/aclacinomycin-A oxidase [EC:1.1.3.45 1.3.3.14]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15949  aknOx; aclacinomycin-N/aclacinomycin-A oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.3  With oxygen as acceptor
    1.1.3.45  aclacinomycin-N oxidase
     K15949  aknOx; aclacinomycin-N/aclacinomycin-A oxidase
  1.3  Acting on the CH-CH group of donors
   1.3.3  With oxygen as acceptor
    1.3.3.14  aclacinomycin-A oxidase
     K15949  aknOx; aclacinomycin-N/aclacinomycin-A oxidase
BRITE hierarchy
Other DBs
RN: R09334 R09335
Genes
AG: ABI15166(aknOx)
TaxonomyKoalaUniProt
Reference
  Authors
Alexeev I, Sultana A, Mantsala P, Niemi J, Schneider G
  Title
Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site.
  Journal
Proc Natl Acad Sci U S A 104:6170-5 (2007)
DOI:10.1073/pnas.0700579104
  Sequence

KEGG   ENZYME: 1.1.3.45Help
Entry
EC 1.1.3.45                 Enzyme                                 

Name
aclacinomycin-N oxidase;
AknOx (ambiguous);
aclacinomycin oxidoreductase (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
aclacinomycin-N:oxygen oxidoreductase
Reaction(IUBMB)
aclacinomycin N + O2 = aclacinomycin A + H2O2 [RN:R09334]
Reaction(KEGG)
Substrate
aclacinomycin N [CPD:C18637];
O2 [CPD:C00007]
Product
aclacinomycin A [CPD:C18638];
H2O2 [CPD:C00027]
Comment
A flavoprotein (FAD). This bifunctional enzyme is a secreted flavin-dependent enzyme that is involved in the modification of the terminal sugar residues in the biosynthesis of aclacinomycins. The enzyme utilizes the same active site to catalyse the oxidation of the rhodinose moiety of aclacinomycin N to the cinerulose A moiety of aclacinomycin A and the oxidation of the latter to the L-aculose moiety of aclacinomycin Y (cf. EC 1.3.3.14, aclacinomycin A oxidase).
History
EC 1.1.3.45 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01130  Biosynthesis of antibiotics
Orthology
K15949  aclacinomycin-N/aclacinomycin-A oxidase
Reference
1  [PMID:17395717]
  Authors
Alexeev I, Sultana A, Mantsala P, Niemi J, Schneider G.
  Title
Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site.
  Journal
Proc Natl Acad Sci U S A 104:6170-5 (2007)
DOI:10.1073/pnas.0700579104
  Sequence
Reference
2  [PMID:17242508]
  Authors
Sultana A, Alexeev I, Kursula I, Mantsala P, Niemi J, Schneider G
  Title
Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning.
  Journal
Acta Crystallogr D Biol Crystallogr 63:149-59 (2007)
DOI:10.1107/S0907444906044271
Other DBs
ExplorEnz - The Enzyme Database: 1.1.3.45
IUBMB Enzyme Nomenclature: 1.1.3.45
ExPASy - ENZYME nomenclature database: 1.1.3.45
BRENDA, the Enzyme Database: 1.1.3.45

KEGG   REACTION: R09334Help
Entry
R09334                      Reaction                               

Name
aclacinomycin N:oxygen oxidoreductase
Definition
Aclacinomycin N + Oxygen <=> Aclacinomycin A + Hydrogen peroxide
Equation
Reaction class
RC00154  C18637_C18638
RC02755  C00007_C00027
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01130  Biosynthesis of antibiotics
Orthology
K15949  aclacinomycin-N/aclacinomycin-A oxidase [EC:1.1.3.45 1.3.3.14]
Other DBs
RHEA: 37426

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