KEGG   ORTHOLOGY: K15950Help
Entry
K15950                      KO                                     

Name
dnrF, rdmE
Definition
aklavinone 12-hydroxylase [EC:1.14.13.180]
Pathway
ko01057  Biosynthesis of type II polyketide products
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01057 Biosynthesis of type II polyketide products
    K15950  dnrF, rdmE; aklavinone 12-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.180  aklavinone 12-hydroxylase
     K15950  dnrF, rdmE; aklavinone 12-hydroxylase
BRITE hierarchy
Other DBs
RN: R06683
Genes
AG: AAA83424(rdmE) AAC43342(dnrF)
TaxonomyKoalaUniProt
Reference
  Authors
Niemi J, Wang Y, Airas K, Ylihonko K, Hakala J, Mantsala P
  Title
Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens.
  Journal
Biochim Biophys Acta 1430:57-64 (1999)
DOI:10.1016/S0167-4838(98)00265-9
  Sequence
Reference
PMID:7773378
  Authors
Filippini S, Solinas MM, Breme U, Schluter MB, Gabellini D, Biamonti G, Colombo AL, Garofano L
  Title
Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression.
  Journal
Microbiology 141 ( Pt 4):1007-16 (1995)
DOI:10.1099/13500872-141-4-1007
  Sequence

KEGG   ENZYME: 1.14.13.180Help
Entry
EC 1.14.13.180              Enzyme                                 

Name
aklavinone 12-hydroxylase;
DnrF;
RdmE;
aklavinone 11-hydroxylase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
aklavinone,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
aklavinone + NADPH + H+ + O2 = epsilon-rhodomycinone + NADP+ + H2O [RN:R06683]
Reaction(KEGG)
Substrate
aklavinone [CPD:C12424];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
epsilon-rhodomycinone [CPD:C12425];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzymes from the Gram-positive bacteria Streptomyces peucetius and Streptomyces purpurascens participate in the biosynthesis of daunorubicin, doxorubicin and rhodomycins. The enzyme from Streptomyces purpurascens is an FAD monooxygenase.
History
EC 1.14.13.180 created 2013
Pathway
ec01057  Biosynthesis of type II polyketide products
ec01130  Biosynthesis of antibiotics
Orthology
K15950  aklavinone 12-hydroxylase
Reference
1  [PMID:7773378]
  Authors
Filippini S, Solinas MM, Breme U, Schluter MB, Gabellini D, Biamonti G, Colombo AL, Garofano L
  Title
Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression.
  Journal
Microbiology 141 ( Pt 4):1007-16 (1995)
DOI:10.1099/13500872-141-4-1007
  Sequence
Reference
2  [PMID:10082933]
  Authors
Niemi J, Wang Y, Airas K, Ylihonko K, Hakala J, Mantsala P
  Title
Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens.
  Journal
Biochim Biophys Acta 1430:57-64 (1999)
DOI:10.1016/S0167-4838(98)00265-9
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.180
IUBMB Enzyme Nomenclature: 1.14.13.180
ExPASy - ENZYME nomenclature database: 1.14.13.180
BRENDA, the Enzyme Database: 1.14.13.180

KEGG   REACTION: R06683Help
Entry
R06683                      Reaction                               

Name
aklavinone,NADPH:oxygen oxidoreductase (12-hydroxylating)
Definition
Aklavinone + NADPH + H+ + Oxygen <=> epsilon-Rhodomycinone + NADP+ + H2O
Equation
Reaction class
RC00001  C00005_C00006
RC00236  C12424_C12425
Enzyme
Pathway
rn01057  Biosynthesis of type II polyketide products
rn01130  Biosynthesis of antibiotics
Orthology
K15950  aklavinone 12-hydroxylase [EC:1.14.13.180]
Other DBs
RHEA: 37838

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