KEGG   ORTHOLOGY: K15989
Entry
K15989            Tight     KO                                     

Name
tylMII
Definition
tylactone mycaminosyltransferase [EC:2.4.1.316]
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01052  Type I polyketide structures
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K15989  tylMII; tylactone mycaminosyltransferase
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K15989  tylMII; tylactone mycaminosyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.316  tylactone mycaminosyltransferase
     K15989  tylMII; tylactone mycaminosyltransferase
Other DBs
RN: R06450
CAZy: GT1
Genes
AG: CAA57472(tylMII)
Reference
  Authors
Rawlings BJ.
  Title
Type I polyketide biosynthesis in bacteria (part B).
  Journal
Nat Prod Rep 18:231-81 (2001)
DOI:10.1039/B100191O
Reference
PMID:9031628
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence
Reference
  Authors
Melancon CE 3rd, Takahashi H, Liu HW
  Title
Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis.
  Journal
J Am Chem Soc 126:16726-7 (2004)
DOI:10.1021/ja043900e
  Sequence

KEGG   ORTHOLOGY: K15990
Entry
K15990            Tight     KO                                     

Name
tylMIII
Definition
tylactone mycaminosyltransferase auxiliary protein
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01052  Type I polyketide structures
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K15990  tylMIII; tylactone mycaminosyltransferase auxiliary protein
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K15990  tylMIII; tylactone mycaminosyltransferase auxiliary protein
Other DBs
RN: R06450
Genes
AG: CAA57471(tylMIII)
Reference
PMID:9031628
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence
Reference
  Authors
Melancon CE 3rd, Takahashi H, Liu HW
  Title
Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis.
  Journal
J Am Chem Soc 126:16726-7 (2004)
DOI:10.1021/ja043900e
  Sequence

KEGG   ENZYME: 2.4.1.316
Entry
EC 2.4.1.316                Enzyme                                 

Name
tylactone mycaminosyltransferase;
tylM2 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
dTDP-alpha-D-mycaminose:tylactone 5-O-beta-D-mycaminosyltransferase
Reaction(IUBMB)
tylactone + dTDP-alpha-D-mycaminose = dTDP + 5-O-beta-D-mycaminosyltylactone [RN:R06450]
Reaction(KEGG)
R06450
Substrate
tylactone [CPD:C12000];
dTDP-alpha-D-mycaminose [CPD:C11933]
Product
dTDP [CPD:C00363];
5-O-beta-D-mycaminosyltylactone [CPD:C12001]
Comment
The enzyme participates in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria. Activity is significantly enhanced by the presence of an accessory protein encoded by the tylM3 gene.
History
EC 2.4.1.316 created 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15989  tylactone mycaminosyltransferase
Reference
1  [PMID:9031628]
  Authors
Gandecha AR, Large SL, Cundliffe E
  Title
Analysis of four tylosin biosynthetic genes from the tylLM region of the Streptomyces fradiae genome.
  Journal
Gene 184:197-203 (1997)
DOI:10.1016/S0378-1119(96)00595-1
  Sequence
Reference
2  [PMID:15612702]
  Authors
Melancon CE 3rd, Takahashi H, Liu HW
  Title
Characterization of tylM3/tylM2 and mydC/mycB pairs required for efficient glycosyltransfer in macrolide antibiotic biosynthesis.
  Journal
J Am Chem Soc 126:16726-7 (2004)
DOI:10.1021/ja043900e
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.316
IUBMB Enzyme Nomenclature: 2.4.1.316
ExPASy - ENZYME nomenclature database: 2.4.1.316
BRENDA, the Enzyme Database: 2.4.1.316

KEGG   REACTION: R06450
Entry
R06450                      Reaction                               

Name
dTDP-alpha-D-mycaminose:tylactone 5-O-beta-D-mycaminosyltransferase
Definition
Tylactone + dTDP-D-mycaminose <=> 5-O-beta-D-Mycaminosyltylactone + dTDP
Equation
Reaction class
RC00005  C00363_C11933
RC00049  C12000_C12001
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00773  Tylosin biosynthesis, methylmalonyl-CoA + malonyl-CoA => tylactone => tylosin
Orthology
K15989  tylactone mycaminosyltransferase [EC:2.4.1.316]
K15990  tylactone mycaminosyltransferase auxiliary protein
Other DBs
RHEA: 21471

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