KEGG   ORTHOLOGY: K16004Help
Entry
K16004                      KO                                     

Name
desVII
Definition
narbonolide/10-deoxymethynolide desosaminyltransferase [EC:2.4.1.277]
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01052  Type I polyketide structures
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00776  Pikromycin/methymycin biosynthesis, methylmalonyl-CoA + malonyl-CoA => narbonolide/10-deoxymethynolide => pikromycin/methymycin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K16004  desVII; narbonolide/10-deoxymethynolide desosaminyltransferase
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K16004  desVII; narbonolide/10-deoxymethynolide desosaminyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.4  Glycosyltransferases
   2.4.1  Hexosyltransferases
    2.4.1.277  10-deoxymethynolide desosaminyltransferase
     K16004  desVII; narbonolide/10-deoxymethynolide desosaminyltransferase
BRITE hierarchy
Other DBs
RN: R06460 R06461
COG: COG1819
CAZy: GT1
Genes
AG: AAC68677(desVII)
TaxonomyKoalaUniProt
Reference
PMID:9770448
  Authors
Xue Y, Zhao L, Liu HW, Sherman DH
  Title
A gene cluster for macrolide antibiotic biosynthesis in Streptomyces venezuelae: architecture of metabolic diversity.
  Journal
Proc Natl Acad Sci U S A 95:12111-6 (1998)
DOI:10.1073/pnas.95.21.12111
  Sequence
Reference
  Authors
Borisova SA, Liu HW
  Title
Characterization of glycosyltransferase DesVII and its auxiliary partner protein DesVIII in the methymycin/picromycin biosynthetic pathway.
  Journal
Biochemistry 49:8071-84 (2010)
DOI:10.1021/bi1007657
  Sequence

KEGG   ORTHOLOGY: K16005Help
Entry
K16005                      KO                                     

Name
desVIII
Definition
narbonolide/10-deoxymethynolide desosaminyltransferase auxiliary protein
Pathway
ko00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ko01052  Type I polyketide structures
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00776  Pikromycin/methymycin biosynthesis, methylmalonyl-CoA + malonyl-CoA => narbonolide/10-deoxymethynolide => pikromycin/methymycin
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01052 Type I polyketide structures
    K16005  desVIII; narbonolide/10-deoxymethynolide desosaminyltransferase auxiliary protein
   00522 Biosynthesis of 12-, 14- and 16-membered macrolides
    K16005  desVIII; narbonolide/10-deoxymethynolide desosaminyltransferase auxiliary protein
BRITE hierarchy
Other DBs
RN: R06460 R06461
Genes
AG: AAC68676(desVIII)
TaxonomyKoalaUniProt
Reference
  Authors
Borisova SA, Liu HW
  Title
Characterization of glycosyltransferase DesVII and its auxiliary partner protein DesVIII in the methymycin/picromycin biosynthetic pathway.
  Journal
Biochemistry 49:8071-84 (2010)
DOI:10.1021/bi1007657
  Sequence
Reference
  Authors
Hong JS, Park SJ, Parajuli N, Park SR, Koh HS, Jung WS, Choi CY, Yoon YJ
  Title
Functional analysis of desVIII homologues involved in glycosylation of macrolide antibiotics by interspecies complementation.
  Journal
Gene 386:123-30 (2007)
DOI:10.1016/j.gene.2006.08.021
  Sequence

KEGG   ENZYME: 2.4.1.277Help
Entry
EC 2.4.1.277                Enzyme                                 

Name
10-deoxymethynolide desosaminyltransferase;
glycosyltransferase DesVII;
DesVII
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose:10-deoxymethynolide 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase
Reaction(IUBMB)
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose + 10-deoxymethynolide = dTDP + 10-deoxymethymycin [RN:R06460]
Reaction(KEGG)
R06460;
(other) R06461
Show
Substrate
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose [CPD:C11911];
10-deoxymethynolide [CPD:C11993]
Product
dTDP [CPD:C00363];
10-deoxymethymycin [CPD:C11994]
Comment
DesVII is the glycosyltransferase responsible for the attachment of dTDP-D-desosamine to 10-deoxymethynolide or narbonolide during the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in the bacterium Streptomyces venezuelae. Activity requires an additional protein partner, DesVIII.
History
EC 2.4.1.277 created 2011, modified 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K16004  narbonolide/10-deoxymethynolide desosaminyltransferase
Reference
1  [PMID:20695498]
  Authors
Borisova SA, Liu HW
  Title
Characterization of glycosyltransferase DesVII and its auxiliary partner protein  DesVIII in the methymycin/picromycin biosynthetic pathway.
  Journal
Biochemistry 49:8071-84 (2010)
DOI:10.1021/bi1007657
  Sequence
Reference
2  [PMID:18548476]
  Authors
Borisova SA, Kim HJ, Pu X, Liu HW
  Title
Glycosylation of acyclic and cyclic aglycone substrates by macrolide glycosyltransferase DesVII/DesVIII: analysis and implications.
  Journal
Chembiochem 9:1554-8 (2008)
DOI:10.1002/cbic.200800155
Reference
3  [PMID:17049185]
  Authors
Hong JS, Park SJ, Parajuli N, Park SR, Koh HS, Jung WS, Choi CY, Yoon YJ
  Title
Functional analysis of desVIII homologues involved in glycosylation of macrolide  antibiotics by interspecies complementation.
  Journal
Gene 386:123-30 (2007)
DOI:10.1016/j.gene.2006.08.021
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.277
IUBMB Enzyme Nomenclature: 2.4.1.277
ExPASy - ENZYME nomenclature database: 2.4.1.277
BRENDA, the Enzyme Database: 2.4.1.277

KEGG   REACTION: R06461Help
Entry
R06461                      Reaction                               

Name
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose:narbonolide 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase
Definition
Narbonolide + dTDP-D-desosamine <=> Narbomycin + dTDP
Equation
Comment
glycosyl transferase,DesVII
Reaction class
RC00005  C00363_C11911
RC00049  C11997_C11998
Enzyme
Pathway
rn00522  Biosynthesis of 12-, 14- and 16-membered macrolides
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00776  Pikromycin/methymycin biosynthesis, methylmalonyl-CoA + malonyl-CoA => narbonolide/10-deoxymethynolide => pikromycin/methymycin
Orthology
K16004  narbonolide/10-deoxymethynolide desosaminyltransferase [EC:2.4.1.277]
K16005  narbonolide/10-deoxymethynolide desosaminyltransferase auxiliary protein

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