KEGG   ORTHOLOGY: K16047
Entry
K16047                      KO                                     

Name
hsaA
Definition
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase [EC:1.14.14.12]
Pathway
ko00984  Steroid degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16047  hsaA; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.12  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
     K16047  hsaA; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Other DBs
RN: R09819
COG: COG1960
Genes
RGL: CS053_01745
VAF: D1115_22760
PRE: PCA10_43690
PCQ: PcP3B5_25640(hsaA_1) PcP3B5_37050(hsaA_3) PcP3B5_37450(hsaA_4)
PPUT: L483_14090
PPUN: PP4_27210
PFE: PSF113_3461 PSF113_3462
PVR: PverR02_11725
PBA: PSEBR_a976
PPUU: PputUW4_01682
PKC: PKB_4323
PSET: THL1_4411
SPL: Spea_2129
SHL: Shal_2101
PHA: PSHAa0877
PTN: PTRA_a1004(hsaA)
PNG: PNIG_a1056(hsaA)
RSY: RSUY_11610(hsaA)
REH: H16_B0638(h16_B0638)
CNC: CNE_2c05830(hsaA)
CGD: CR3_0799(hsaA)
BCJ: BCAM1647
BCEN: DM39_6202
BCEO: I35_5507
BAM: Bamb_5026
BCT: GEM_4008
BCED: DM42_6311
BCON: NL30_03950
BTEI: WS51_05860
BPSL: WS57_05845
BMEC: WJ16_26975
BGO: BM43_6910
BFN: OI25_4616
CABA: SBC2_48740(hsaA_1)
BAV: BAV0595
CTES: O987_07755
DAR: Daro_0902
BJA: bll6096
BRS: S23_21790
BRAD: BF49_5537
NWI: Nwi_0937
NHA: Nham_3406
XAU: Xaut_0933
SNO: Snov_0210
CSE: Cseg_1728
SPHM: G432_01990
STAX: MC45_16520
SPHI: TS85_13285
SSAN: NX02_12720
SSY: SLG_18380
SPHB: EP837_03391(hsaA)
SMIC: SmB9_33370
MTU: Rv3570c(hsaA)
MTC: MT3675
MRA: MRA_3609
MTUR: CFBS_3787
MTD: UDA_3570c
MTUC: J113_25000
MTUE: J114_19095
MTUH: I917_25085
MTUL: TBHG_03511
MTUT: HKBT1_3772
MTUU: HKBT2_3781
MBO: BQ2027_MB3601C(hsaa)
MBB: BCG_3635c
MBT: JTY_3636
MBX: BCGT_3437
MAF: MAF_35830
MMIC: RN08_3947
MCQ: BN44_110065(hsaA)
MCV: BN43_90071(hsaA)
MCX: BN42_90068(hsaA)
MCZ: BN45_100084(hsaA)
MLP: MLM_0735
MHAD: B586_04445
MSHG: MSG_00802(hsaA_1) MSG_02472 MSG_04563(hsaA_2)
MPHL: MPHLCCUG_00491(hsaA_1) MPHLCCUG_01764(hsaA_2) MPHLCCUG_02628(hsaA_4) MPHLCCUG_03656(hsaA_5) MPHLCCUG_03657(hsaA_6)
MTHN: 4412656_00960(hsaA_1) 4412656_01361(hsaA_3) 4412656_04094(hsaA_5)
MHAS: MHAS_00635(hsaA_1) MHAS_02456(hsaA_2) MHAS_03779(hsaA_3)
MAB: MAB_0584
MSAL: DSM43276_00265(hsaA_1) DSM43276_00502(hsaA_2)
MTER: 4434518_02237(hsaA_2) 4434518_03729(hsaA_3) 4434518_04155(hsaA_4)
ASD: AS9A_0924
NFR: ERS450000_00305(hsaA_1) ERS450000_00680(hsaA_2) ERS450000_00694(hsaA_3) ERS450000_01850(hsaA_6) ERS450000_03762(hsaA_8)
RHA: RHA1_ro04539(hsaA)
RHB: NY08_3127
RHU: A3Q40_00865(hsaA)
RRT: 4535765_00582(hsaA_1) 4535765_00630(hsaA_2) 4535765_00632(hsaA_3) 4535765_01483(hsaA_4) 4535765_04054(hsaA_6) 4535765_04572(hsaA_7) 4535765_04586(hsaA_8)
GBR: Gbro_0920
GOR: KTR9_0820(hsaA)
GRU: GCWB2_04175(hsaA1) GCWB2_08185(hsaA2)
GOM: D7316_02455(hsaA_1) D7316_02566(hsaA_2)
TPR: Tpau_3845
SRT: Srot_0299
SHY: SHJG_0396
SALS: SLNWT_2215
SFI: SFUL_4664
SALU: DC74_7445
SALL: SAZ_38430
STRE: GZL_08548
STRM: M444_37895
SMAL: SMALA_8231
SALJ: SMD11_0294
SLX: SLAV_27430(hsaA1)
SGE: DWG14_00038(hsaA_1) DWG14_00204(hsaA_2) DWG14_08423(hsaA_3)
NDK: I601_0579(hsaA_1) I601_3470(hsaA_2)
SRO: Sros_6977
AMD: AMED_2522
AJA: AJAP_26405(hsaA)
AMYY: YIM_14450(hsaA1) YIM_23025(hsaA2) YIM_36670(hsaA6) YIM_40880(hsaA8)
PAUT: Pdca_45790
KAL: KALB_5124
AHG: AHOG_16455(hsaA1)
SAQ: Sare_2879
TBI: Tbis_1633
CWO: Cwoe_2133
TRO: trd_A0928
 » show all
Reference
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J Biol Chem 285:22264-75 (2010)
DOI:10.1074/jbc.M109.099028
  Sequence
[mtu:Rv3570c]
Reference
  Authors
Ouellet H, Johnston JB, de Montellano PR
  Title
Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis.
  Journal
Trends Microbiol 19:530-9 (2011)
DOI:10.1016/j.tim.2011.07.009

KEGG   ORTHOLOGY: K16048
Entry
K16048                      KO                                     

Name
hsaB
Definition
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase reductase component [EC:1.5.1.-]
Pathway
ko00984  Steroid degradation
ko01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16048  hsaB; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.-  
     K16048  hsaB; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase reductase component
Other DBs
RN: R09819
COG: COG1853
Genes
THEH: G7079_10275
RGL: CS053_01750
VAF: D1115_22455
PMY: Pmen_3440 Pmen_3451
PRE: PCA10_14160 PCA10_14530
PCQ: PcP3B5_03810(hsaB_1) PcP3B5_03920(hsaB_2) PcP3B5_37060(nphA2_1) PcP3B5_37440(nphA2_2)
PPF: Pput_2083 Pput_2088 Pput_3055
PPG: PputGB1_2261 PputGB1_2266 PputGB1_3091
PPW: PputW619_2256 PputW619_2261
PPT: PPS_3137 PPS_3142
PFL: PFL_3512
PPRC: PFLCHA0_c35530(hsaB)
PPRO: PPC_3666
SPL: Spea_2119
SHL: Shal_2091
PHA: PSHAa2140(tesA)
PTN: PTRA_a2593(hsaB)
PNG: PNIG_a2787(hsaB)
PLG: NCTC10937_03408(hsaB_1) NCTC10937_03417(hsaB_2)
BAV: BAV1593
AMIM: MIM_c33700
CTES: O987_07750
CFU: CFU_2819(tesA)
SCL: sce8995
PLA: Plav_0994
AVI: Avi_5077
MDI: METDI5651
MEX: Mext_4599
MCH: Mchl_5060
RBM: TEF_19910
CID: P73_2005
HNE: HNE_1255
SGI: SGRAN_3245(hsaB)
SPHU: SPPYR_0643
STAX: MC45_16625
SSY: SLG_03810
SPMI: K663_16395
SPHB: EP837_01489(hsaB)
SPHR: BSY17_1464
MAG: amb2577
MAGN: WV31_06375
MTU: Rv3567c(hsaB)
MTC: MT3672
MRA: MRA_3606
MTUR: CFBS_3784
MTD: UDA_3567c
MTUB: MT7199_3630(hsaB)
MTUC: J113_24985
MTUE: J114_19080
MTUL: TBHG_03508
MTUT: HKBT1_3769
MTUU: HKBT2_3778
MBO: BQ2027_MB3598C(hsab)
MBB: BCG_3632c
MBT: JTY_3633
MBX: BCGT_3434
MAF: MAF_35800
MMIC: RN08_3944
MPA: MAP_0500
MAO: MAP4_3367
MAVI: RC58_16700
MAVU: RE97_16735
MAV: MAV_0594
MIT: OCO_05030
MIA: OCU_05070
MID: MIP_00945
MYO: OEM_05090
MIR: OCQ_05180
MLP: MLM_0738
MUL: MUL_4138
MMC: Mmcs_4706
MKM: Mkms_4792
MJL: Mjls_5091
MMI: MMAR_5062
MMM: W7S_02455
MHAD: B586_04460
MSHG: MSG_04560(hsaB)
MPHL: MPHLCCUG_00494(hsaB_1)
MVQ: MYVA_5215
MTHN: 4412656_04091(hsaB_2)
MHAS: MHAS_03776(hsaB_1)
MAB: MAB_0586
MABB: MASS_0555
MCHE: BB28_02795
MSTE: MSTE_00543
MSAL: DSM43276_00504(hsaB_1)
MTER: 4434518_03727(hsaB_2)
ASD: AS9A_0927
COA: DR71_752(hsaB)
NFR: ERS450000_03765(hsaB_2) ERS450000_05818(hsaB_3)
RHA: RHA1_ro04542(hsaB)
RRT: 4535765_00585(hsaB_1) 4535765_00634(hsaB_2) 4535765_04041(hsaB_5)
GOR: KTR9_0822(hsaB) KTR9_4330
GRU: GCWB2_04185(hsaB1) GCWB2_08360(hsaB2)
GOM: D7316_02453(hsaB_1) D7316_02571(hsaB_2)
SGR: SGR_3042
SGB: WQO_20525
SCT: SCAT_2368
SBH: SBI_06291
SHY: SHJG_3986
SVE: SVEN_4243
SALS: SLNWT_0921
STRE: GZL_05149
SLD: T261_4762
STRM: M444_15715
SPRI: SPRI_4256
SRW: TUE45_00680(hsaB_2) TUE45_04284(hsaB_4)
SLE: sle_01310(sle_01310) sle_62720(sle_62720)
STRD: NI25_18930
SLAU: SLA_4342
SALJ: SMD11_3928
SLX: SLAV_16820(hsaB1) SLAV_27190(hsaB2) SLAV_27445(hsaB3)
SGE: DWG14_00207(hsaB_1) DWG14_04767(hsaB_4)
SRJ: SRO_4177
ACH: Achl_1781
MPH: MLP_43280
NCA: Noca_2773
NDK: I601_0582(hsaB_1) I601_3467(hsaB_2)
NAL: B005_2517
SRO: Sros_2092
FAL: FRAAL1664
AMD: AMED_2525
AOI: AORI_2487
AJA: AJAP_26390(hsaB)
AMYY: YIM_14465(hsaB1)
PSEA: WY02_09245
PSEH: XF36_14940
AMI: Amir_4448
KAL: KALB_5121
MIL: ML5_6168
ACTN: L083_3831
CAI: Caci_8752
TBI: Tbis_1628
CWO: Cwoe_2144
RCA: Rcas_4187
LIE: LIF_A2361
LIC: LIC_11147(ntaB)
LIS: LIL_12482
LBJ: LBJ_0960
LBL: LBL_2073
LST: LSS_02834
MTP: Mthe_0466
HHI: HAH_5125
 » show all
Reference
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J Biol Chem 285:22264-75 (2010)
DOI:10.1074/jbc.M109.099028
  Sequence
[mtu:Rv3567c]
Reference
  Authors
Ouellet H, Johnston JB, de Montellano PR
  Title
Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis.
  Journal
Trends Microbiol 19:530-9 (2011)
DOI:10.1016/j.tim.2011.07.009

KEGG   ENZYME: 1.14.14.12
Entry
EC 1.14.14.12               Enzyme                                 

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase;
HsaA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O [RN:R09819]
Reaction(KEGG)
R09819
Substrate
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C19944];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. It can use either FADH or FMNH2 as flavin cofactor. The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
History
EC 1.14.14.12 created 2011
Pathway
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16047  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Genes
RGL: CS053_01745
VAF: D1115_22760
PRE: PCA10_43690
PCQ: PcP3B5_25640(hsaA_1) PcP3B5_37050(hsaA_3) PcP3B5_37450(hsaA_4)
PPUT: L483_14090
PPUN: PP4_27210
PFE: PSF113_3461 PSF113_3462
PVR: PverR02_11725
PBA: PSEBR_a976
PPUU: PputUW4_01682
PKC: PKB_4323
PSET: THL1_4411
SPL: Spea_2129
SHL: Shal_2101
PHA: PSHAa0877
PTN: PTRA_a1004(hsaA)
PNG: PNIG_a1056(hsaA)
RSY: RSUY_11610(hsaA)
REH: H16_B0638(h16_B0638)
CNC: CNE_2c05830(hsaA)
CGD: CR3_0799(hsaA)
BCJ: BCAM1647
BCEN: DM39_6202
BCEO: I35_5507
BAM: Bamb_5026
BCT: GEM_4008
BCED: DM42_6311
BCON: NL30_03950
BTEI: WS51_05860
BPSL: WS57_05845
BMEC: WJ16_26975
BGO: BM43_6910
BFN: OI25_4616
CABA: SBC2_48740(hsaA_1)
BAV: BAV0595
CTES: O987_07755
DAR: Daro_0902
BJA: bll6096
BRS: S23_21790
BRAD: BF49_5537
NWI: Nwi_0937
NHA: Nham_3406
XAU: Xaut_0933
SNO: Snov_0210
CSE: Cseg_1728
SPHM: G432_01990
STAX: MC45_16520
SPHI: TS85_13285
SSAN: NX02_12720
SSY: SLG_18380
SPHB: EP837_03391(hsaA)
SMIC: SmB9_33370
MTU: Rv3570c(hsaA)
MTC: MT3675
MRA: MRA_3609
MTUR: CFBS_3787
MTD: UDA_3570c
MTUC: J113_25000
MTUE: J114_19095
MTUH: I917_25085
MTUL: TBHG_03511
MTUT: HKBT1_3772
MTUU: HKBT2_3781
MBO: BQ2027_MB3601C(hsaa)
MBB: BCG_3635c
MBT: JTY_3636
MBX: BCGT_3437
MAF: MAF_35830
MMIC: RN08_3947
MCQ: BN44_110065(hsaA)
MCV: BN43_90071(hsaA)
MCX: BN42_90068(hsaA)
MCZ: BN45_100084(hsaA)
MLP: MLM_0735
MHAD: B586_04445
MSHG: MSG_00802(hsaA_1) MSG_02472 MSG_04563(hsaA_2)
MPHL: MPHLCCUG_00491(hsaA_1) MPHLCCUG_01764(hsaA_2) MPHLCCUG_02628(hsaA_4) MPHLCCUG_03656(hsaA_5) MPHLCCUG_03657(hsaA_6)
MTHN: 4412656_00960(hsaA_1) 4412656_01361(hsaA_3) 4412656_04094(hsaA_5)
MHAS: MHAS_00635(hsaA_1) MHAS_02456(hsaA_2) MHAS_03779(hsaA_3)
MAB: MAB_0584
MSAL: DSM43276_00265(hsaA_1) DSM43276_00502(hsaA_2)
MTER: 4434518_02237(hsaA_2) 4434518_03729(hsaA_3) 4434518_04155(hsaA_4)
ASD: AS9A_0924
NFR: ERS450000_00305(hsaA_1) ERS450000_00680(hsaA_2) ERS450000_00694(hsaA_3) ERS450000_01850(hsaA_6) ERS450000_03762(hsaA_8)
RHA: RHA1_ro04539(hsaA)
RHB: NY08_3127
RHU: A3Q40_00865(hsaA)
RRT: 4535765_00582(hsaA_1) 4535765_00630(hsaA_2) 4535765_00632(hsaA_3) 4535765_01483(hsaA_4) 4535765_04054(hsaA_6) 4535765_04572(hsaA_7) 4535765_04586(hsaA_8)
GBR: Gbro_0920
GOR: KTR9_0820(hsaA)
GRU: GCWB2_04175(hsaA1) GCWB2_08185(hsaA2)
GOM: D7316_02455(hsaA_1) D7316_02566(hsaA_2)
TPR: Tpau_3845
SRT: Srot_0299
SHY: SHJG_0396
SALS: SLNWT_2215
SFI: SFUL_4664
SALU: DC74_7445
SALL: SAZ_38430
STRE: GZL_08548
STRM: M444_37895
SMAL: SMALA_8231
SALJ: SMD11_0294
SLX: SLAV_27430(hsaA1)
SGE: DWG14_00038(hsaA_1) DWG14_00204(hsaA_2) DWG14_08423(hsaA_3)
NDK: I601_0579(hsaA_1) I601_3470(hsaA_2)
SRO: Sros_6977
AMD: AMED_2522
AJA: AJAP_26405(hsaA)
AMYY: YIM_14450(hsaA1) YIM_23025(hsaA2) YIM_36670(hsaA6) YIM_40880(hsaA8)
PAUT: Pdca_45790
KAL: KALB_5124
AHG: AHOG_16455(hsaA1)
SAQ: Sare_2879
TBI: Tbis_1633
CWO: Cwoe_2133
TRO: trd_A0928
 » show all
Reference
1  [PMID:20448045]
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J Biol Chem 285:22264-75 (2010)
DOI:10.1074/jbc.M109.099028
  Sequence
[mtu:Rv3570c]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.12
IUBMB Enzyme Nomenclature: 1.14.14.12
ExPASy - ENZYME nomenclature database: 1.14.14.12
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.14.12
BRENDA, the Enzyme Database: 1.14.14.12

KEGG   REACTION: R09819
Entry
R09819                      Reaction                               

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Definition
3-Hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + Reduced FMN + Oxygen <=> 3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O
Equation
Comment
FMN is re-reduced by NADH (see R05705)
Reaction class
RC00126  C00061_C01847
RC00236  C04793_C19944
Enzyme
Pathway
rn00984  Steroid degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K16047  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase [EC:1.14.14.12]
K16048  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase reductase component [EC:1.5.1.-]
Other DBs
RHEA: 31734

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