KEGG   ORTHOLOGY: K16049
Entry
K16049                      KO                                     

Name
hsaC
Definition
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase [EC:1.13.11.25]
Pathway
ko00984  Steroid degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16049  hsaC; 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.13  Acting on single donors with incorporation of molecular oxygen (oxygenases)
   1.13.11  With incorporation of two atoms of oxygen
    1.13.11.25  3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
     K16049  hsaC; 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
Other DBs
RN: R04597
COG: COG0346
GO: 0047071
Genes
VAF: D1115_22660
PRE: PCA10_13380
PPUT: L483_14230
PPUN: PP4_26930
PVR: PverR02_11720
PPUU: PputUW4_01687
PKC: PKB_4267(hsaC)
PFZ: AV641_10715
PLQ: AA042_19960
PPSY: AOC04_08020
PSET: THL1_1410
PSIL: PMA3_11800
SPL: Spea_2153
SHL: Shal_2124
PHA: PSHAa0900(tesB)
PNG: PNIG_a1084(hsaC)
GPB: HDN1F_27360(bphC1)
RIN: ACS15_4384(hsaC)
REH: H16_B0654(bphC)
CNC: CNE_2c06010(bphC)
CTI: RALTA_B0496(bphC)
BCJ: BCAM1637
BCEN: DM39_6216(hsaC)
BCEO: I35_5498
BCT: GEM_4079
BCED: DM42_6321(hsaC)
BCON: NL30_03995
BTEI: WS51_05820
BPSL: WS57_05915
BMEC: WJ16_26910
BFN: OI25_4609
CABA: SBC2_48810(hsaC)
CTES: O987_07285
SPHK: SKP52_00920(bphC1)
SPHM: G432_04775
SSAN: NX02_14735
SPHB: EP837_03390(hsaC)
SPHT: K426_15855
SMIC: SmB9_10410(hsaC)
MTU: Rv3568c(hsaC)
MTC: MT3673
MRA: MRA_3607(bphC)
MTUR: CFBS_3785(bphC)
MTO: MTCTRI2_3633(bphC)
MTD: UDA_3568c(bphC)
MTN: ERDMAN_3915(bphC)
MTUC: J113_24990
MTUE: J114_19085
MTUL: TBHG_03509
MTUT: HKBT1_3770(bphC)
MTUU: HKBT2_3779(bphC)
MTQ: HKBS1_3782(bphC)
MBO: BQ2027_MB3599C(hsac)
MBB: BCG_3633c(bphC)
MBT: JTY_3634(bphC)
MBM: BCGMEX_3631c(bphC)
MBX: BCGT_3435
MAF: MAF_35810(bphC)
MMIC: RN08_3945
MCE: MCAN_35811(bphC)
MCQ: BN44_110063(hsaC)
MCV: BN43_90069(hsaC)
MCX: BN42_90066(hsaC)
MCZ: BN45_100082(hsaC)
MPA: MAP_0499
MAO: MAP4_3368
MAVI: RC58_16705
MAVU: RE97_16740
MAV: MAV_0593
MUL: MUL_4139(bphC)
MMI: MMAR_5063(bphC)
MMAE: MMARE11_48780(bphC)
MLI: MULP_05321(bphC)
MHAD: B586_04455
MSHG: MSG_02471 MSG_04561(hsaC)
MSG: MSMEI_2817(bphC) MSMEI_5875(bphC)
MPHL: MPHLCCUG_00493(hsaC_1) MPHLCCUG_02627(hsaC_2)
MTHN: 4412656_00959(bphC) 4412656_04092(hsaC)
MHAS: MHAS_02457(hsaC_1) MHAS_03777(hsaC_2)
MAB: MAB_0585
MSTE: MSTE_00542(hsaC)
MSAL: DSM43276_00503(hsaC)
MJD: JDM601_3853(bphC) JDM601_4250(bphC)
MTER: 4434518_03728(bphC)
ASD: AS9A_0926
NFR: ERS450000_03764(hsaC_1) ERS450000_05815(hsaC_2)
NNO: NONO_c05030(hsaC1) NONO_c44720 NONO_c58760(hsaC2)
NSR: NS506_01260(hsaC) NS506_06778(etbC)
RHA: RHA1_ro04541(hsaC)
RRT: 4535765_00584(hsaC_1) 4535765_00631(hsaC_2) 4535765_01490(hsaC_3) 4535765_04055(hsaC_4)
GBR: Gbro_0921
GOR: KTR9_0821(hsaC)
GRU: GCWB2_04180(hsaC)
GOM: D7316_02454(hsaC_1) D7316_02467(hsaC_2) D7316_02567(hsaC_3)
TPR: Tpau_3843
SALU: DC74_7447
SALL: SAZ_38440
SMAL: SMALA_8448
SLX: SLAV_27440(hsaC)
NCA: Noca_2774
NDK: I601_0578(hsaC_1) I601_3468(hsaC_2)
AMD: AMED_2524
AMN: RAM_12830
AMM: AMES_2496
AMZ: B737_2497
AOI: AORI_2486(bphC)
AJA: AJAP_26395(hsaC)
AMYY: YIM_14460(hsaC1) YIM_36675(hsaC2)
KAL: KALB_5122
SAQ: Sare_2877
 » show all
Reference
  Authors
Van der Geize R, Yam K, Heuser T, Wilbrink MH, Hara H, Anderton MC, Sim E, Dijkhuizen L, Davies JE, Mohn WW, Eltis LD
  Title
A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.
  Journal
Proc Natl Acad Sci U S A 104:1947-52 (2007)
DOI:10.1073/pnas.0605728104
Reference
  Authors
Ouellet H, Johnston JB, de Montellano PR
  Title
Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis.
  Journal
Trends Microbiol 19:530-9 (2011)
DOI:10.1016/j.tim.2011.07.009

KEGG   ENZYME: 1.13.11.25
Entry
EC 1.13.11.25               Enzyme                                 

Name
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase;
steroid 4,5-dioxygenase;
3-alkylcatechol 2,3-dioxygenase;
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (decyclizing)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
Sysname
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (ring-opening)
Reaction(IUBMB)
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + O2 = 3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [RN:R04597]
Reaction(KEGG)
R04597
Substrate
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
O2 [CPD:C00007]
Product
3-hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate [CPD:C04844]
Comment
Requires Fe2+. Also acts on 3-isopropylcatechol and 3-tert-butyl-5-methylcatechol.
History
EC 1.13.11.25 created 1972
Pathway
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16049  3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase
Genes
VAF: D1115_22660
PRE: PCA10_13380
PPUT: L483_14230
PPUN: PP4_26930
PVR: PverR02_11720
PPUU: PputUW4_01687
PKC: PKB_4267(hsaC)
PFZ: AV641_10715
PLQ: AA042_19960
PPSY: AOC04_08020
PSET: THL1_1410
PSIL: PMA3_11800
SPL: Spea_2153
SHL: Shal_2124
PHA: PSHAa0900(tesB)
PNG: PNIG_a1084(hsaC)
GPB: HDN1F_27360(bphC1)
RIN: ACS15_4384(hsaC)
REH: H16_B0654(bphC)
CNC: CNE_2c06010(bphC)
CTI: RALTA_B0496(bphC)
BCJ: BCAM1637
BCEN: DM39_6216(hsaC)
BCEO: I35_5498
BCT: GEM_4079
BCED: DM42_6321(hsaC)
BCON: NL30_03995
BTEI: WS51_05820
BPSL: WS57_05915
BMEC: WJ16_26910
BFN: OI25_4609
CABA: SBC2_48810(hsaC)
CTES: O987_07285
SPHK: SKP52_00920(bphC1)
SPHM: G432_04775
SSAN: NX02_14735
SPHB: EP837_03390(hsaC)
SPHT: K426_15855
SMIC: SmB9_10410(hsaC)
MTU: Rv3568c(hsaC)
MTC: MT3673
MRA: MRA_3607(bphC)
MTUR: CFBS_3785(bphC)
MTO: MTCTRI2_3633(bphC)
MTD: UDA_3568c(bphC)
MTN: ERDMAN_3915(bphC)
MTUC: J113_24990
MTUE: J114_19085
MTUL: TBHG_03509
MTUT: HKBT1_3770(bphC)
MTUU: HKBT2_3779(bphC)
MTQ: HKBS1_3782(bphC)
MBO: BQ2027_MB3599C(hsac)
MBB: BCG_3633c(bphC)
MBT: JTY_3634(bphC)
MBM: BCGMEX_3631c(bphC)
MBX: BCGT_3435
MAF: MAF_35810(bphC)
MMIC: RN08_3945
MCE: MCAN_35811(bphC)
MCQ: BN44_110063(hsaC)
MCV: BN43_90069(hsaC)
MCX: BN42_90066(hsaC)
MCZ: BN45_100082(hsaC)
MPA: MAP_0499
MAO: MAP4_3368
MAVI: RC58_16705
MAVU: RE97_16740
MAV: MAV_0593
MUL: MUL_4139(bphC)
MMI: MMAR_5063(bphC)
MMAE: MMARE11_48780(bphC)
MLI: MULP_05321(bphC)
MHAD: B586_04455
MSHG: MSG_02471 MSG_04561(hsaC)
MSG: MSMEI_2817(bphC) MSMEI_5875(bphC)
MPHL: MPHLCCUG_00493(hsaC_1) MPHLCCUG_02627(hsaC_2)
MTHN: 4412656_00959(bphC) 4412656_04092(hsaC)
MHAS: MHAS_02457(hsaC_1) MHAS_03777(hsaC_2)
MAB: MAB_0585
MSTE: MSTE_00542(hsaC)
MSAL: DSM43276_00503(hsaC)
MJD: JDM601_3853(bphC) JDM601_4250(bphC)
MTER: 4434518_03728(bphC)
ASD: AS9A_0926
NFR: ERS450000_03764(hsaC_1) ERS450000_05815(hsaC_2)
NNO: NONO_c05030(hsaC1) NONO_c44720 NONO_c58760(hsaC2)
NSR: NS506_01260(hsaC) NS506_06778(etbC)
RHA: RHA1_ro04541(hsaC)
RRT: 4535765_00584(hsaC_1) 4535765_00631(hsaC_2) 4535765_01490(hsaC_3) 4535765_04055(hsaC_4)
GBR: Gbro_0921
GOR: KTR9_0821(hsaC)
GRU: GCWB2_04180(hsaC)
GOM: D7316_02454(hsaC_1) D7316_02467(hsaC_2) D7316_02567(hsaC_3)
TPR: Tpau_3843
SALU: DC74_7447
SALL: SAZ_38440
SMAL: SMALA_8448
SLX: SLAV_27440(hsaC)
NCA: Noca_2774
NDK: I601_0578(hsaC_1) I601_3468(hsaC_2)
AMD: AMED_2524
AMN: RAM_12830
AMM: AMES_2496
AMZ: B737_2497
AOI: AORI_2486(bphC)
AJA: AJAP_26395(hsaC)
AMYY: YIM_14460(hsaC1) YIM_36675(hsaC2)
KAL: KALB_5122
SAQ: Sare_2877
 » show all
Reference
1  [PMID:5908121]
  Authors
Gibson DT, Wang KC, Sih CJ, Whitlock H Jr.
  Title
Mechanisms of steroid oxidation by microorganisms. IX. On the mechanism of ring A cleavage in the degradation of 9,10-seco steroids by microorganisms.
  Journal
J Biol Chem 241:551-9 (1966)
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.25
IUBMB Enzyme Nomenclature: 1.13.11.25
ExPASy - ENZYME nomenclature database: 1.13.11.25
UM-BBD (Biocatalysis/Biodegradation Database): 1.13.11.25
BRENDA, the Enzyme Database: 1.13.11.25
CAS: 37256-63-6

KEGG   REACTION: R04597
Entry
R04597                      Reaction                               

Name
3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione:oxygen 4,5-oxidoreductase (decyclizing)
Definition
3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + Oxygen <=> 3-Hydroxy-5,9,17-trioxo-4,5:9,10-disecoandrosta-1(10),2-dien-4-oate
Equation
Reaction class
RC01610  C04793_C04844
Enzyme
Pathway
rn00984  Steroid degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Orthology
K16049  3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase [EC:1.13.11.25]
Other DBs
RHEA: 43899

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