KEGG   ORTHOLOGY: K16157
Entry
K16157                      KO                                     

Name
mmoX
Definition
methane monooxygenase component A alpha chain [EC:1.14.13.25]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16157  mmoX; methane monooxygenase component A alpha chain
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.25  methane monooxygenase (soluble)
     K16157  mmoX; methane monooxygenase component A alpha chain
Other DBs
RN: R01142
GO: 0015049
Genes
MCA: MCA1194(mmoX)
MMT: Metme_1649
MSL: Msil_1262
MBRY: B1812_14140
MMI: MMAR_0131
MCB: Mycch_5395 Mycch_5875 Mycch_5901
MRH: MycrhN_3049
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ORTHOLOGY: K16158
Entry
K16158                      KO                                     

Name
mmoY
Definition
methane monooxygenase component A beta chain [EC:1.14.13.25]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16158  mmoY; methane monooxygenase component A beta chain
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.25  methane monooxygenase (soluble)
     K16158  mmoY; methane monooxygenase component A beta chain
Other DBs
RN: R01142
GO: 0015049
Genes
MCA: MCA1195(mmoY)
METU: GNH96_11975
MMT: Metme_1650
MSL: Msil_1263
MBRY: B1812_14135
MMI: MMAR_0130
MCB: Mycch_5900
MRH: MycrhN_3050
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ORTHOLOGY: K16159
Entry
K16159                      KO                                     

Name
mmoZ
Definition
methane monooxygenase component A gamma chain [EC:1.14.13.25]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16159  mmoZ; methane monooxygenase component A gamma chain
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.25  methane monooxygenase (soluble)
     K16159  mmoZ; methane monooxygenase component A gamma chain
Other DBs
RN: R01142
GO: 0015049
Genes
MCA: MCA1198(mmoZ)
METU: GNH96_11965
MMT: Metme_1652
MSL: Msil_1265
MBRY: B1812_14125
MCB: Mycch_5897
MRH: MycrhN_3054
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ORTHOLOGY: K16160
Entry
K16160                      KO                                     

Name
mmoB
Definition
methane monooxygenase regulatory protein B
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16160  mmoB; methane monooxygenase regulatory protein B
Other DBs
RN: R01142
Genes
MCA: MCA1196(mmoB)
METU: GNH96_11970
MMT: Metme_1651
MSL: Msil_1264
MBRY: B1812_14130
MCB: Mycch_5899
MRH: MycrhN_3051
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ORTHOLOGY: K16161
Entry
K16161                      KO                                     

Name
mmoC
Definition
methane monooxygenase component C [EC:1.14.13.25]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16161  mmoC; methane monooxygenase component C
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.25  methane monooxygenase (soluble)
     K16161  mmoC; methane monooxygenase component C
Other DBs
RN: R01142
COG: COG0543 COG0633
GO: 0015049
Genes
MCA: MCA1200(mmoC)
MMT: Metme_1654
MSL: Msil_1267
MBRY: B1812_14115
MCB: Mycch_5898
MRH: MycrhN_3053
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ORTHOLOGY: K16162
Entry
K16162                      KO                                     

Name
mmoD
Definition
methane monooxygenase component D
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
ko01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16162  mmoD; methane monooxygenase component D
Other DBs
RN: R01142
Genes
MCA: MCA1199(mmoD)
METU: GNH96_11960(mmoD)
MMT: Metme_1653
MSL: Msil_1266
MBRY: B1812_14120
MRH: MycrhN_3052
Reference
  Authors
Murrell JC, Gilbert B, McDonald IR
  Title
Molecular biology and regulation of methane monooxygenase.
  Journal
Arch Microbiol 173:325-32 (2000)
DOI:10.1007/s002030000158

KEGG   ENZYME: 1.14.13.25
Entry
EC 1.14.13.25               Enzyme                                 

Name
methane monooxygenase (soluble);
methane hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
methane,NAD(P)H:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
methane + NAD(P)H + H+ + O2 = methanol + NAD(P)+ + H2O [RN:R01142 R01143]
Reaction(KEGG)
Substrate
methane [CPD:C01438];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
methanol [CPD:C00132];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
The enzyme is soluble, in contrast to the particulate enzyme, EC 1.14.18.3. Broad specificity; many alkanes can be hydroxylated, and alkenes are converted into the corresponding epoxides; CO is oxidized to CO2, ammonia is oxidized to hydroxylamine, and some aromatic compounds and cyclic alkanes can also be hydroxylated, but more slowly.
History
EC 1.14.13.25 created 1984, modified 2011
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16157  methane monooxygenase component A alpha chain
K16158  methane monooxygenase component A beta chain
K16159  methane monooxygenase component A gamma chain
K16161  methane monooxygenase component C
Genes
MCA: MCA1194(mmoX) MCA1195(mmoY) MCA1198(mmoZ) MCA1200(mmoC)
METU: GNH96_11965 GNH96_11975
MMT: Metme_1649 Metme_1650 Metme_1652 Metme_1654
MSL: Msil_1262 Msil_1263 Msil_1265 Msil_1267
MBRY: B1812_14115 B1812_14125 B1812_14135 B1812_14140
MMI: MMAR_0130 MMAR_0131
MCB: Mycch_5395 Mycch_5875 Mycch_5897 Mycch_5898 Mycch_5900 Mycch_5901
MRH: MycrhN_3049 MycrhN_3050 MycrhN_3053 MycrhN_3054
Reference
1  [PMID:411486]
  Authors
Colby J, Stirling DI, Dalton H.
  Title
The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds.
  Journal
Biochem J 165:395-402 (1977)
DOI:10.1042/bj1650395
Reference
2  [PMID:6870854]
  Authors
Hyman MR, Wood PM.
  Title
Methane oxidation by Nitrosomonas europaea.
  Journal
Biochem J 212:31-7 (1983)
DOI:10.1042/bj2120031
Reference
3  [PMID:572296]
  Authors
Stirling DI, Dalton H.
  Title
Properties of the methane mono-oxygenase from extracts of Methylosinus trichosporium OB3b and evidence for its similarity to the enzyme from Methylococcus capsulatus (Bath).
  Journal
Eur J Biochem 96:205-12 (1979)
DOI:10.1111/j.1432-1033.1979.tb13030.x
Reference
4  [PMID:15544]
  Authors
Tonge GM, Harrison DE, Higgins IJ.
  Title
Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b.
  Journal
Biochem J 161:333-44 (1977)
DOI:10.1042/bj1610333
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.25
IUBMB Enzyme Nomenclature: 1.14.13.25
ExPASy - ENZYME nomenclature database: 1.14.13.25
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.13.25
BRENDA, the Enzyme Database: 1.14.13.25
CAS: 51961-97-8

KEGG   REACTION: R01142
Entry
R01142                      Reaction                               

Name
Methane,NADH:oxygen oxidoreductase (hydroxylating)
Definition
Methane + Oxygen + NADH + H+ <=> Methanol + NAD+ + H2O
Equation
Comment
NADPH (see R01143)
Reaction class
RC00001  C00003_C00004
RC00173  C00132_C01438
Enzyme
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
rn01220  Degradation of aromatic compounds
Module
M00174  Methane oxidation, methanotroph, methane => formaldehyde
Orthology
K16157  methane monooxygenase component A alpha chain [EC:1.14.13.25]
K16158  methane monooxygenase component A beta chain [EC:1.14.13.25]
K16159  methane monooxygenase component A gamma chain [EC:1.14.13.25]
K16160  methane monooxygenase regulatory protein B
K16161  methane monooxygenase component C [EC:1.14.13.25]
K16162  methane monooxygenase component D
Other DBs
RHEA: 13640

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