KEGG   ORTHOLOGY: K16176
Entry
K16176                      KO                                     

Name
mtmB
Definition
methylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.248]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16176  mtmB; methylamine---corrinoid protein Co-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.248  methylamine---corrinoid protein Co-methyltransferase
     K16176  mtmB; methylamine---corrinoid protein Co-methyltransferase
Other DBs
RN: R09998 R10014
Genes
SDF: ACG33_12615
DOE: DENOEST_1155 DENOEST_1170
DEO: CAY53_02475
DML: Dmul_02380(mtmB)
CTHD: CDO33_18585
DAE: Dtox_0025
ELIM: B2M23_05155 B2M23_05160
AAR: Acear_1719
SRI: SELR_27700(mtmB)
MANA: MAMMFC1_01966 MAMMFC1_01967
MAC: MA_0144(mtmB1) MA_2972(mtmB2)
MMA: MM_1436(mtmB) MM_1437(mtmB) MM_3335(mtmB) MM_3336(mtmB)
MTHR: MSTHT_1926
MTHE: MSTHC_1357
MMH: Mmah_1399
MPY: Mpsy_1674 Mpsy_1675(mtmB1) Mpsy_1677(mtmB2) Mpsy_1711(mtmB3)
MEAR: Mpt1_c05730(mtmB11) Mpt1_c05760(mtmB13)
TPE: Tpen_1211
 » show all
Reference
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Reference
PMID:9642198
  Authors
Burke SA, Lo SL, Krzycki JA
  Title
Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.
  Journal
J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998
  Sequence

KEGG   ENZYME: 2.1.1.248
Entry
EC 2.1.1.248                Enzyme                                 

Name
methylamine---corrinoid protein Co-methyltransferase;
mtmB (gene name);
monomethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
monomethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
methylamine + a [Co(I) methylamine-specific corrinoid protein] = a [methyl-Co(III) methylamine-specific corrinoid protein] + NH3 [RN:R10014]
Reaction(KEGG)
R10014;
(other) R09998
Substrate
methylamine [CPD:C00218];
[Co(I) methylamine-specific corrinoid protein]
Product
[methyl-Co(III) methylamine-specific corrinoid protein];
NH3 [CPD:C00014]
Comment
The enzyme, which catalyses the transfer of a methyl group from methylamine to a methylamine-specific corrinoid protein (MtmC), is involved in methanogenesis from methylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.248 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K16176  methylamine---corrinoid protein Co-methyltransferase
Genes
SDF: ACG33_12615
DOE: DENOEST_1155 DENOEST_1170
DEO: CAY53_02475
DML: Dmul_02380(mtmB)
CTHD: CDO33_18585
DAE: Dtox_0025
ELIM: B2M23_05155 B2M23_05160
AAR: Acear_1719
SRI: SELR_27700(mtmB)
MANA: MAMMFC1_01966 MAMMFC1_01967
MAC: MA_0144(mtmB1) MA_2972(mtmB2)
MMA: MM_1436(mtmB) MM_1437(mtmB) MM_3335(mtmB) MM_3336(mtmB)
MTHR: MSTHT_1926
MTHE: MSTHC_1357
MMH: Mmah_1399
MPY: Mpsy_1674 Mpsy_1675(mtmB1) Mpsy_1677(mtmB2) Mpsy_1711(mtmB3)
MEAR: Mpt1_c05730(mtmB11) Mpt1_c05760(mtmB13)
TPE: Tpen_1211
 » show all
Reference
1  [PMID:9195968]
  Authors
Burke SA, Krzycki JA
  Title
Reconstitution of Monomethylamine:Coenzyme M methyl transfer with a corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 272:16570-7 (1997)
DOI:10.1074/jbc.272.26.16570
  Sequence
Reference
2  [PMID:9642198]
  Authors
Burke SA, Lo SL, Krzycki JA
  Title
Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.
  Journal
J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998
  Sequence
Reference
3  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.248
IUBMB Enzyme Nomenclature: 2.1.1.248
ExPASy - ENZYME nomenclature database: 2.1.1.248
BRENDA, the Enzyme Database: 2.1.1.248

KEGG   REACTION: R09998
Entry
R09998                      Reaction                               

Name
methylamine:coenzyme M methyltransferase
Definition
Methylamine + Coenzyme M <=> Ammonia + 2-(Methylthio)ethanesulfonate
Equation
Comment
two-step reaction (see R10014+R10000)
Reaction class
RC00035  C03576_C03920
RC02985  C00218_C03920
Enzyme
2.1.1.247       2.1.1.248
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
K16176  methylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.248]
K16177  monomethylamine corrinoid protein

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