KEGG   ORTHOLOGY: K16178
Entry
K16178                      KO                                     

Name
mtbB
Definition
dimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.249]
Pathway
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16178  mtbB; dimethylamine---corrinoid protein Co-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.249  dimethylamine---corrinoid protein Co-methyltransferase
     K16178  mtbB; dimethylamine---corrinoid protein Co-methyltransferase
Other DBs
RN: R09999 R10015
Genes
AMIC: Ami3637_11500
TPZ: Tph_c27720(mtbB2)
AAR: Acear_1273
MBY: MSBRM_0457 MSBRM_0926 MSBRM_0957
MBW: MSBRW_1129 MSBRW_2236 MSBRW_3223
MBAR: MSBR2_1469 MSBR2_1924
MBAK: MSBR3_0479 MSBR3_0618 MSBR3_1955
MAC: MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)
MMA: MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)
MMJ: MSMAS_1746 MSMAS_2635 MSMAS_2980
MPY: Mpsy_1680(mtbB) Mpsy_1702(mtbB3)
MARC: AR505_1332
AG: AAD14633(mtbB1)
 » show all
Reference
  Authors
Soares JA, Zhang L, Pitsch RL, Kleinholz NM, Jones RB, Wolff JJ, Amster J, Green-Church KB, Krzycki JA
  Title
The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases.
  Journal
J Biol Chem 280:36962-9 (2005)
DOI:10.1074/jbc.M506402200

KEGG   ENZYME: 2.1.1.249
Entry
EC 2.1.1.249                Enzyme                                 

Name
dimethylamine---corrinoid protein Co-methyltransferase;
mtbB (gene name);
dimethylamine methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
Reaction(IUBMB)
dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] = a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine [RN:R10015]
Reaction(KEGG)
R10015;
(other) R09999
Substrate
dimethylamine [CPD:C00543];
[Co(I) dimethylamine-specific corrinoid protein]
Product
[methyl-Co(III) dimethylamine-specific corrinoid protein];
methylamine [CPD:C00218]
Comment
The enzyme, which catalyses the transfer of a methyl group from dimethylamine to a dimethylamine-specific corrinoid protein (MtbC), is involved in methanogenesis from dimethylamine. The enzyme contains the unusual amino acid pyrrolysine [3]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
History
EC 2.1.1.249 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K16178  dimethylamine---corrinoid protein Co-methyltransferase
Genes
AMIC: Ami3637_11500
TPZ: Tph_c27720(mtbB2)
AAR: Acear_1273
MBY: MSBRM_0457 MSBRM_0926 MSBRM_0957
MBW: MSBRW_1129 MSBRW_2236 MSBRW_3223
MBAR: MSBR2_1469 MSBR2_1924
MBAK: MSBR3_0479 MSBR3_0618 MSBR3_1955
MAC: MA_0532(mtbB) MA_0933(mtbB) MA_2425(mtbB)
MMA: MM_1693(mtbB) MM_2051(mtbB) MM_2962(mtbB)
MMJ: MSMAS_1746 MSMAS_2635 MSMAS_2980
MPY: Mpsy_1680(mtbB) Mpsy_1702(mtbB3)
MARC: AR505_1332
 » show all
Reference
1  [PMID:9654067]
  Authors
Wassenaar RW, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and characterization of dimethylamine:5-hydroxybenzimidazolyl-cobamide methyltransferase from Methanosarcina barkeri Fusaro.
  Journal
Eur J Biochem 253:692-7 (1998)
DOI:10.1046/j.1432-1327.1998.2530692.x
Reference
2  [PMID:10852929]
  Authors
Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA
  Title
Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.
  Journal
J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199
  Sequence
Reference
3  [PMID:15450490]
  Authors
Krzycki JA
  Title
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.
  Journal
Curr Opin Chem Biol 8:484-91 (2004)
DOI:10.1016/j.cbpa.2004.08.012
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.249
IUBMB Enzyme Nomenclature: 2.1.1.249
ExPASy - ENZYME nomenclature database: 2.1.1.249
BRENDA, the Enzyme Database: 2.1.1.249

KEGG   REACTION: R09999
Entry
R09999                      Reaction                               

Name
dimethylamine:coenzyme M methyltransferase
Definition
Dimethylamine + Coenzyme M <=> 2-(Methylthio)ethanesulfonate + Methylamine
Equation
Comment
two-step reaction (see R10015+R10000)
Reaction class
RC00035  C03576_C03920
RC00556  C00218_C00543
RC02986  C00543_C03920
Enzyme
2.1.1.247       2.1.1.249
Pathway
rn00680  Methane metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00563  Methanogenesis, methylamine/dimethylamine/trimethylamine => methane
Orthology
K14082  [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase [EC:2.1.1.247]
K16178  dimethylamine---corrinoid protein Co-methyltransferase [EC:2.1.1.249]
K16179  dimethylamine corrinoid protein

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