KEGG   ORTHOLOGY: K16180
Entry
K16180                      KO                                     

Name
pylB
Definition
methylornithine synthase [EC:5.4.99.58]
Pathway
ko00300  Lysine biosynthesis
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K16180  pylB; methylornithine synthase
Enzymes [BR:ko01000]
 5. Isomerases
  5.4  Intramolecular transferases
   5.4.99  Transferring other groups
    5.4.99.58  methylornithine synthase
     K16180  pylB; methylornithine synthase
Other DBs
RN: R10010
COG: COG0502
Genes
DAT: HRM2_15240(bioB2)
DOV: DSCO28_05090
DSY: DSY4958
DHD: Dhaf_4857
DDH: Desde_0574
DAE: Dtox_0028
DGI: Desgi_3925
TJR: TherJR_0023
DOR: Desor_1148 Desor_1623 Desor_3055
DMI: Desmer_1592 Desmer_2996
AMIJ: EQM06_09285(pylB)
AMIC: Ami3637_11535(pylB)
ABUT: Ami103574_12095(pylB)
MANA: MAMMFC1_01955(bioB_2)
MMIL: sm9_0396(hcgA)
MEAR: Mpt1_c05690(pylB2)
MARC: AR505_1324
MBAR: MSBR2_2225
MBAK: MSBR3_2276
MAC: MA_0154(bioB)
MMA: MM_1444
MMAC: MSMAC_3242
METM: MSMTP_3120
MTHR: MSTHT_1933
MTHE: MSTHC_1350
MHOR: MSHOH_3966
MFZ: AOB57_008965(pylB)
MBU: Mbur_2085(pylB)
MMET: MCMEM_0284
MMH: Mmah_0282
MPY: Mpsy_1722(bioB)
MZI: HWN40_00970(pylB)
 » show all
Reference
  Authors
Gaston MA, Jiang R, Krzycki JA
  Title
Functional context, biosynthesis, and genetic encoding of pyrrolysine.
  Journal
Curr Opin Microbiol 14:342-9 (2011)
DOI:10.1016/j.mib.2011.04.001

KEGG   ENZYME: 5.4.99.58
Entry
EC 5.4.99.58                Enzyme                                 

Name
methylornithine synthase;
PylB
Class
Isomerases;
Intramolecular transferases;
Transferring other groups
Sysname
L-lysine carboxy-aminomethylmutase
Reaction(IUBMB)
L-lysine = (3R)-3-methyl-D-ornithine [RN:R10010]
Reaction(KEGG)
R10010
Substrate
L-lysine [CPD:C00047]
Product
(3R)-3-methyl-D-ornithine [CPD:C20277]
Comment
The enzyme is a member of the superfamily of S-adenosyl-L-methionine-dependent radical (radical AdoMet) enzymes. Binds a [4Fe-4S] cluster that is coordinated by 3 cysteines and an exchangeable S-adenosyl-L-methionine molecule. The reaction is part of the biosynthesis pathway of pyrrolysine, a naturally occurring amino acid found in some archaeal methyltransferases.
History
EC 5.4.99.58 created 2012
Pathway
ec00300  Lysine biosynthesis
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16180  methylornithine synthase
Genes
DAT: HRM2_15240(bioB2)
DOV: DSCO28_05090
DSY: DSY4958
DHD: Dhaf_4857
DDH: Desde_0574
DAE: Dtox_0028
DGI: Desgi_3925
TJR: TherJR_0023
DOR: Desor_1148 Desor_1623 Desor_3055
DMI: Desmer_1592 Desmer_2996
AMIJ: EQM06_09285(pylB)
AMIC: Ami3637_11535(pylB)
ABUT: Ami103574_12095(pylB)
MANA: MAMMFC1_01955(bioB_2)
MMIL: sm9_0396(hcgA)
MEAR: Mpt1_c05690(pylB2)
MARC: AR505_1324
MBAR: MSBR2_2225
MBAK: MSBR3_2276
MAC: MA_0154(bioB)
MMA: MM_1444
MMAC: MSMAC_3242
METM: MSMTP_3120
MTHR: MSTHT_1933
MTHE: MSTHC_1350
MHOR: MSHOH_3966
MFZ: AOB57_008965(pylB)
MBU: Mbur_2085(pylB)
MMET: MCMEM_0284
MMH: Mmah_0282
MPY: Mpsy_1722(bioB)
MZI: HWN40_00970(pylB)
 » show all
Reference
1  [PMID:21455182]
  Authors
Gaston MA, Zhang L, Green-Church KB, Krzycki JA
  Title
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from  lysine.
  Journal
Nature 471:647-50 (2011)
DOI:10.1038/nature09918
  Sequence
[mac:MA_0154]
Reference
2  [PMID:22095926]
  Authors
Quitterer F, List A, Eisenreich W, Bacher A, Groll M
  Title
Crystal structure of methylornithine synthase (PylB): insights into the pyrrolysine biosynthesis.
  Journal
Angew Chem Int Ed Engl 51:1339-42 (2012)
DOI:10.1002/anie.201106765
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.4.99.58
IUBMB Enzyme Nomenclature: 5.4.99.58
ExPASy - ENZYME nomenclature database: 5.4.99.58
BRENDA, the Enzyme Database: 5.4.99.58

KEGG   REACTION: R10010
Entry
R10010                      Reaction                               

Name
L-lysine carboxy-aminomethylmutase
Definition
L-Lysine <=> (2R,3R)-3-Methylornithine
Equation
Comment
radical SAM
Reaction class
RC02995  C00047_C20277
Enzyme
Pathway
rn00300  Lysine biosynthesis
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K16180  methylornithine synthase [EC:5.4.99.58]
Other DBs
RHEA: 32762

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