KEGG   ORTHOLOGY: K16431
Entry
K16431                      KO                                     

Name
cepH, sgcC3, mdpC3, kedY3
Definition
FAD-dependent halogenase [EC:1.14.19.-]
Pathway
ko01055  Biosynthesis of vancomycin group antibiotics
ko01059  Biosynthesis of enediyne antibiotics
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00827  C-1027 beta-amino acid moiety biosynthesis, tyrosine => 3-chloro-4,5-dihydroxy-beta-phenylalanyl-PCP
M00832  Kedarcidin 2-aza-3-chloro-beta-tyrosine moiety biosynthesis, azatyrosine => 2-aza-3-chloro-beta-tyrosyl-PCP
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01059 Biosynthesis of enediyne antibiotics
    K16431  cepH, sgcC3, mdpC3, kedY3; FAD-dependent halogenase
   01055 Biosynthesis of vancomycin group antibiotics
    K16431  cepH, sgcC3, mdpC3, kedY3; FAD-dependent halogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.-  
     K16431  cepH, sgcC3, mdpC3, kedY3; FAD-dependent halogenase
Other DBs
RN: R11368 R11393
COG: COG0644
Genes
NTP: CRH09_30915
GPO: GPOL_c23630
GOM: D7316_00962(lodB)
SGB: WQO_33760
SBH: SBI_00676
SRC: M271_00350 M271_09130
STRE: GZL_07290
SPAV: Spa2297_32975
STRT: A8713_00440
SLS: SLINC_7264
SFK: KY5_1050c
STRR: EKD16_14345(lodB)
AMYY: YIM_16050(lodB)
ACTI: UA75_18105
ACAD: UA74_17580
SAQ: Sare_2119
AG: ABY66006(mdpC3) AFV52210(kedY3)
 » show all
Reference
  Authors
Xu L, Huang H, Wei W, Zhong Y, Tang B, Yuan H, Zhu L, Huang W, Ge M, Yang S, Zheng H, Jiang W, Chen D, Zhao GP, Zhao W
  Title
Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis.
  Journal
BMC Genomics 15:363 (2014)
DOI:10.1186/1471-2164-15-363
  Sequence
[aoi:AORI_1485]
Reference
  Authors
Lin S, Van Lanen SG, Shen B
  Title
Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027.
  Journal
J Am Chem Soc 129:12432-8 (2007)
DOI:10.1021/ja072311g
Reference
PMID:12183628 (sgcC3)
  Authors
Liu W, Christenson SD, Standage S, Shen B
  Title
Biosynthesis of the enediyne antitumor antibiotic C-1027.
  Journal
Science 297:1170-3 (2002)
DOI:10.1126/science.1072110
  Sequence
[sgb:WQO_33760]

KEGG   ORTHOLOGY: K21185
Entry
K21185                      KO                                     

Name
sgcE6, mdpE6, kedE6
Definition
flavin reductase
Pathway
ko01059  Biosynthesis of enediyne antibiotics
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00827  C-1027 beta-amino acid moiety biosynthesis, tyrosine => 3-chloro-4,5-dihydroxy-beta-phenylalanyl-PCP
M00832  Kedarcidin 2-aza-3-chloro-beta-tyrosine moiety biosynthesis, azatyrosine => 2-aza-3-chloro-beta-tyrosyl-PCP
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   01059 Biosynthesis of enediyne antibiotics
    K21185  sgcE6, mdpE6, kedE6; flavin reductase
Other DBs
RN: R11368 R11393
COG: COG1853
Genes
BGF: BC1003_1446
BXB: DR64_207(pheA2)
BGE: BC1002_1302
BPY: Bphyt_1723
PSPW: BJG93_06360
PARB: CJU94_16560
PCJ: CUJ87_06945
PMEG: FNZ07_21825
RHF: EUB48_08835
BRS: S23_66080
BRAD: BF49_3457
TMR: Tmar_0112
SGB: WQO_33985
SFI: SFUL_4665
STRM: M444_37995
SRJ: SRO_1836
ART: Arth_2570
LMOI: VV02_20445
KFL: Kfla_5529
NDA: Ndas_1186
SRO: Sros_6997
FAL: FRAAL6129(bfl)
NML: Namu_5120
AMD: AMED_6862
AMN: RAM_35210
AMM: AMES_6757
AMZ: B737_6757
AOI: AORI_1842
AMYY: YIM_40775(hsaB2)
AORI: SD37_32000
PDX: Psed_3092
ACTI: UA75_19065
ACAD: UA74_18575
AHG: AHOG_16575(nphA2)
ALO: CRK57496
SAQ: Sare_0553
CAI: Caci_6349
RXY: Rxyl_0953
AG: AAQ17112(mdpE6) AFV52147(kedE6)
 » show all
Reference
PMID:12183628 (sgcE6)
  Authors
Liu W, Christenson SD, Standage S, Shen B
  Title
Biosynthesis of the enediyne antitumor antibiotic C-1027.
  Journal
Science 297:1170-3 (2002)
DOI:10.1126/science.1072110
  Sequence
[sgb:WQO_33985]
Reference
  Authors
Van Lanen SG, Lin S, Horsman GP, Shen B
  Title
Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027.
  Journal
FEMS Microbiol Lett 300:237-41 (2009)
DOI:10.1111/j.1574-6968.2009.01802.x
Reference
  Authors
Chang CY, Lohman JR, Cao H, Tan K, Rudolf JD, Ma M, Xu W, Bingman CA, Yennamalli RM, Bigelow L, Babnigg G, Yan X, Joachimiak A, Phillips GN Jr, Shen B
  Title
Crystal Structures of SgcE6 and SgcC, the Two-Component Monooxygenase That Catalyzes Hydroxylation of a Carrier Protein-Tethered Substrate during the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus.
  Journal
Biochemistry 55:5142-54 (2016)
DOI:10.1021/acs.biochem.6b00713

KEGG   REACTION: R11393
Entry
R11393                      Reaction                               

Name
(R)-2-aza-beta-tyrosyl-[pcp]:FADH2 oxidoreductase
Definition
(R)-2-Aza-beta-tyrosyl-[pcp] + FADH2 + Cl- + Oxygen + H+ <=> (R)-2-Aza-3-chloro-beta-tyrosyl-[pcp] + FAD + 2 H2O
Equation
Reaction class
RC03431  C21327_C21328
Enzyme
1.14.19.-
Pathway
rn01059  Biosynthesis of enediyne antibiotics
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00832  Kedarcidin 2-aza-3-chloro-beta-tyrosine moiety biosynthesis, azatyrosine => 2-aza-3-chloro-beta-tyrosyl-PCP
Orthology
K16431  FAD-dependent halogenase [EC:1.14.19.-]
K21185  flavin reductase

DBGET integrated database retrieval system