KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
09108 Metabolism of cofactors and vitamins
00780 Biotin metabolism
K16593 bioI, CYP107H; pimeloyl-[acyl-carrier protein] synthase
09180 Brite Hierarchies
09181 Protein families: metabolism
00199 Cytochrome P450
K16593 bioI, CYP107H; pimeloyl-[acyl-carrier protein] synthase
Enzymes [BR:ko01000]
1. Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
1.14.14.46 pimeloyl-[acyl-carrier protein] synthase
K16593 bioI, CYP107H; pimeloyl-[acyl-carrier protein] synthase
Cytochrome P450 [BR:ko00199]
Cytochrome P450, bacteria type
CYP107 family
K16593 bioI, CYP107H; pimeloyl-[acyl-carrier protein] synthase
Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis.
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
A cytochrome P-450 (heme-thiolate) protein. The enzyme catalyses an oxidative C-C bond cleavage of long-chain acyl-[acyl-carrier protein]s of various lengths to generate pimeloyl-[acyl-carrier protein], an intermediate in the biosynthesis of biotin. The preferred substrate of the enzyme from the bacterium Bacillus subtilis is palmitoyl-[acyl-carrier protein] which then gives heptanal as the alkanal. The mechanism is similar to EC 1.14.15.6, cholesterol monooxygenase (side-chain-cleaving), followed by a hydroxylation step, which may occur spontaneously [2].
History
EC 1.14.14.46 created 2013 as EC 1.14.15.12, transferred 2017 to EC 1.14.14.46
Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis.
Enzymatic reactions [BR:br08201]
1. Oxidoreductase reactions
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
1.14.14.46
R10123 Long-chain acyl-[acyl-carrier protein] + 2 Reduced flavodoxin + 3 Oxygen <=> Pimeloyl-[acyl-carrier protein] + n-Alkanal + 2 Oxidized flavodoxin + 3 H2O