KEGG   ORTHOLOGY: K16877Help
Entry
K16877                      KO                                     

Name
hmfA
Definition
2-furoyl-CoA dehydrogenase large subunit [EC:1.3.99.8]
Pathway
ko00365  Furfural degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00365 Furfural degradation
    K16877  hmfA; 2-furoyl-CoA dehydrogenase large subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.99  With unknown physiological acceptors
    1.3.99.8  2-furoyl-CoA dehydrogenase
     K16877  hmfA; 2-furoyl-CoA dehydrogenase large subunit
BRITE hierarchy
Other DBs
RN: R02987
GO: 0047542
Genes
PPSE: BN5_2298(hmfA)
PMAN: OU5_0889
PSTT: CH92_11530
PPUU: PputUW4_02625
PSIL: PMA3_09770
ADI: B5T_02845
RPI: Rpic_2052
RPF: Rpic12D_1744
RPJ: N234_37060
REU: Reut_C5890
REH: H16_A1703(coxL3)
BXE: Bxe_A2073
BXB: DR64_4228
BPH: Bphy_6673
BFN: OI25_3188
AMIM: MIM_c34440
ODI: ODI_R0528
HPSE: HPF_15720(cdhA)
BJA: blr3533(cutL)
RPC: RPC_0848
MET: M446_1301
MNO: Mnod_3296
MOR: MOC_3639
MAQU: Maq22A_c13995(coxL)
DSH: Dshi_3719(coxL)
ACR: Acry_0668
AZL: AZL_a11020(xdhB)
GKA: GKP21
GGH: GHH_c13560(cutL)
ANM: GFC28_297
PDX: Psed_5933
PSEA: WY02_08310
PAUT: Pdca_67730(cutL_4)
STO: STK_00930
PYR: P186_1143
POG: Pogu_1990
AG: ADE20399(hmfA)
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ
  Title
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.
  Journal
Proc Natl Acad Sci U S A 107:4919-24 (2010)
DOI:10.1073/pnas.0913039107
  Sequence

KEGG   ORTHOLOGY: K16878Help
Entry
K16878                      KO                                     

Name
hmfB
Definition
2-furoyl-CoA dehydrogenase FAD binding subunit [EC:1.3.99.8]
Pathway
ko00365  Furfural degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00365 Furfural degradation
    K16878  hmfB; 2-furoyl-CoA dehydrogenase FAD binding subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.99  With unknown physiological acceptors
    1.3.99.8  2-furoyl-CoA dehydrogenase
     K16878  hmfB; 2-furoyl-CoA dehydrogenase FAD binding subunit
BRITE hierarchy
Other DBs
RN: R02987
GO: 0047542
Genes
PPSE: BN5_2299(hmfB)
PMAN: OU5_0888
PSTT: CH92_11535
PPUU: PputUW4_02624
PSIL: PMA3_09775
ADI: B5T_02846
RPI: Rpic_2053
RPF: Rpic12D_1745
RPJ: N234_37065
REU: Reut_C5889
REH: H16_A1702(h16_A1702)
BXE: Bxe_A2074
BXB: DR64_4229
BPH: Bphy_6672
BFN: OI25_3189
AMIM: MIM_c34430
ODI: ODI_R0529
HPSE: HPF_15725(kdhA)
BJA: blr3534(cutM)
RPC: RPC_0849
MET: M446_1302
MNO: Mnod_3297
MOR: MOC_3638
MAQU: Maq22A_c13990(coxM)
DSH: Dshi_3720(coxM)
ACR: Acry_0669
AZL: AZL_a11010(cutM)
GKA: GKP19
AG: ADE20400(hmfB)
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ
  Title
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.
  Journal
Proc Natl Acad Sci U S A 107:4919-24 (2010)
DOI:10.1073/pnas.0913039107
  Sequence

KEGG   ORTHOLOGY: K16879Help
Entry
K16879                      KO                                     

Name
hmfC
Definition
2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit [EC:1.3.99.8]
Pathway
ko00365  Furfural degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00365 Furfural degradation
    K16879  hmfC; 2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.99  With unknown physiological acceptors
    1.3.99.8  2-furoyl-CoA dehydrogenase
     K16879  hmfC; 2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit
BRITE hierarchy
Other DBs
RN: R02987
GO: 0047542
Genes
PPSE: BN5_2300(hmfC)
PMAN: OU5_0887
PSTT: CH92_11540
PPUU: PputUW4_02623
PSIL: PMA3_09780
ADI: B5T_02847
RPI: Rpic_2054
RPF: Rpic12D_1746
RPJ: N234_37070
REU: Reut_C5888
REH: H16_A1701(coxS3)
BXE: Bxe_A2075
BXB: DR64_4230(qorS)
BPH: Bphy_6671
BFN: OI25_3190
AMIM: MIM_c34420
ODI: ODI_R0530
HPSE: HPF_15730(cdhC)
BJA: blr3535
RPC: RPC_0850
MET: M446_1303
MNO: Mnod_3298
MOR: MOC_3637
MAQU: Maq22A_c13985(coxS)
DSH: Dshi_3721(coxS)
ACR: Acry_0670
GKA: GKP18
AG: ADE20401(hmfC)
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Koopman F, Wierckx N, de Winde JH, Ruijssenaars HJ
  Title
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14.
  Journal
Proc Natl Acad Sci U S A 107:4919-24 (2010)
DOI:10.1073/pnas.0913039107
  Sequence

KEGG   ENZYME: 1.3.99.8Help
Entry
EC 1.3.99.8                 Enzyme                                 

Name
2-furoyl-CoA dehydrogenase;
furoyl-CoA hydroxylase;
2-furoyl coenzyme A hydroxylase;
2-furoyl coenzyme A dehydrogenase;
2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
BRITE hierarchy
Sysname
2-furoyl-CoA:acceptor 5-oxidoreductase (hydroxylating)
Reaction(IUBMB)
2-furoyl-CoA + H2O + acceptor = S-(5-hydroxy-2-furoyl)-CoA + reduced acceptor [RN:R02987]
Reaction(KEGG)
Substrate
2-furoyl-CoA [CPD:C00845];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Product
S-(5-hydroxy-2-furoyl)-CoA [CPD:C03724];
reduced acceptor [CPD:C00030]
Comment
A copper protein. The oxygen atom of the -OH produced is derived from water, not O2; the actual oxidative step is probably dehydrogenation of a hydrated form -CHOH-CH2- to -C(OH)=CH-, which tautomerizes non-enzymically to -CO-CH2-, giving (5-oxo-4,5-dihydro-2-furoyl)-CoA. Methylene blue, nitro blue, tetrazolium and a membrane fraction from Pseudomonas putida can act as acceptors.
History
EC 1.3.99.8 created 1976
Pathway
ec00365  Furfural degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K16877  2-furoyl-CoA dehydrogenase large subunit
K16878  2-furoyl-CoA dehydrogenase FAD binding subunit
K16879  2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit
Genes
PPSE: BN5_2298(hmfA) BN5_2299(hmfB) BN5_2300(hmfC)
PMAN: OU5_0887 OU5_0888 OU5_0889
PSTT: CH92_11530 CH92_11535 CH92_11540
PPUU: PputUW4_02623 PputUW4_02624 PputUW4_02625
PSIL: PMA3_09770 PMA3_09775 PMA3_09780
ADI: B5T_02845 B5T_02846 B5T_02847
RPI: Rpic_2052 Rpic_2053 Rpic_2054
RPF: Rpic12D_1744 Rpic12D_1745 Rpic12D_1746
RPJ: N234_37060 N234_37065 N234_37070
REU: Reut_C5888 Reut_C5889 Reut_C5890
REH: H16_A1701(coxS3) H16_A1702(h16_A1702) H16_A1703(coxL3)
HPSE: HPF_15720(cdhA) HPF_15725(kdhA) HPF_15730(cdhC)
BJA: blr3533(cutL) blr3534(cutM) blr3535
MAQU: Maq22A_c13985(coxS) Maq22A_c13990(coxM) Maq22A_c13995(coxL)
DSH: Dshi_3719(coxL) Dshi_3720(coxM) Dshi_3721(coxS)
AZL: AZL_a11000 AZL_a11010(cutM) AZL_a11020(xdhB)
ANM: GFC28_297
PDX: Psed_5933
PSEA: WY02_08310
PAUT: Pdca_67730(cutL_4)
STO: STK_00930
PYR: P186_1143
POG: Pogu_1990
 » show all
Taxonomy
Reference
1  [PMID:4655411]
  Authors
Kitcher JP, Trudgill PW, Rees JS.
  Title
Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2.
  Journal
Biochem J 130:121-32 (1972)
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.8
IUBMB Enzyme Nomenclature: 1.3.99.8
ExPASy - ENZYME nomenclature database: 1.3.99.8
UM-BBD (Biocatalysis/Biodegradation Database): 1.3.99.8
BRENDA, the Enzyme Database: 1.3.99.8
CAS: 9068-18-2

KEGG   REACTION: R02987Help
Entry
R02987                      Reaction                               

Name
2-furoyl-CoA:(acceptor) 5-oxidoreductase (hydroxylating)
Definition
2-Furoyl-CoA + Acceptor + H2O <=> S-(5-Hydroxy-2-furoyl)-CoA + Reduced acceptor
Equation
Comment
possibly intermediate
Reaction class
RC01856  C00845_C03724
Enzyme
Pathway
rn00365  Furfural degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Orthology
K16877  2-furoyl-CoA dehydrogenase large subunit [EC:1.3.99.8]
K16878  2-furoyl-CoA dehydrogenase FAD binding subunit [EC:1.3.99.8]
K16879  2-furoyl-CoA dehydrogenase 2Fe-2S iron sulfur subunit [EC:1.3.99.8]
Other DBs
RHEA: 21483

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