KEGG   ORTHOLOGY: K17450Help
Entry
K17450                      KO                                     

Name
ACO2
Definition
homoaconitase [EC:4.2.1.-]
Pathway
ko00300  Lysine biosynthesis
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01130  Biosynthesis of antibiotics
ko01210  2-Oxocarboxylic acid metabolism
ko01230  Biosynthesis of amino acids
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
M00433  Lysine biosynthesis, 2-oxoglutarate => 2-oxoadipate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K17450  ACO2; homoaconitase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.-  
     K17450  ACO2; homoaconitase
BRITE hierarchy
Other DBs
RN: R03444
COG: COG1048
Genes
QSU: 111985251 112036931
SCE: YJL200C(ACO2)
AGO: AGOS_AFR629W
ERC: Ecym_8142
KLA: KLLA0_C03432g
KMX: KLMA_10153(ACO2a)
LTH: KLTH0F03586g
VPO: Kpol_2001p56
ZRO: ZYRO0C17974g
CGR: CAGL0F02431g
NCS: NCAS_0F00160(NCAS0F00160)
NDI: NDAI_0K02930(NDAI0K02930)
TPF: TPHA_0D04460(TPHA0D04460)
TBL: TBLA_0J00580(TBLA0J00580)
TDL: TDEL_0C05660(TDEL0C05660)
KAF: KAFR_0F01540(KAFR0F01540)
PIC: PICST_90724(ACO2)
SPAA: SPAPADRAFT_133954(ACO2)
CAL: CAALFM_CR05790CA(ACO2)
SLB: AWJ20_2939(ACO2)
NCR: NCU04280
NTE: NEUTE1DRAFT75176(NEUTE1DRAFT_75176)
MGR: MGG_08584
SSCK: SPSK_08585
MAW: MAC_00341
MAJ: MAA_02899
CMT: CCM_01579
BFU: BCIN_04g04360(Bcaco2)
MBE: MBM_04948
ANI: AN3894.2
ANG: ANI_1_470084(An09g03870)
ABE: ARB_00016
TVE: TRV_07808
PTE: PTT_12730
ZTR: MYCGRDRAFT_88201(ACO2)
CNE: CNM01770
CNB: CNBM1650
MRR: Moror_157
ABP: AGABI1DRAFT66587(AGABI1DRAFT_66587)
ABV: AGABI2DRAFT62721(AGABI2DRAFT_62721)
MRT: MRET_2215
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Fazius F, Shelest E, Gebhardt P, Brock M
  Title
The fungal alpha-aminoadipate pathway for lysine biosynthesis requires two enzymes of the aconitase family for the isomerization of homocitrate to homoisocitrate.
  Journal
Mol Microbiol 86:1508-30 (2012)
DOI:10.1111/mmi.12076

KEGG   ORTHOLOGY: K16792Help
Entry
K16792                      KO                                     

Name
aksD
Definition
methanogen homoaconitase large subunit [EC:4.2.1.114]
Pathway
ko00300  Lysine biosynthesis
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01130  Biosynthesis of antibiotics
ko01210  2-Oxocarboxylic acid metabolism
ko01230  Biosynthesis of amino acids
Module
M00433  Lysine biosynthesis, 2-oxoglutarate => 2-oxoadipate
M00608  2-Oxocarboxylic acid chain extension, 2-oxoglutarate => 2-oxoadipate => 2-oxopimelate => 2-oxosuberate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16792  aksD; methanogen homoaconitase large subunit
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K16792  aksD; methanogen homoaconitase large subunit
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.114  methanogen homoaconitase
     K16792  aksD; methanogen homoaconitase large subunit
BRITE hierarchy
Other DBs
RN: R03444 R04371 R09720 R10391 R10392 R10393 R10394 R10395 R10396
COG: COG0065
Genes
OBJ: EIO64_04565
RRS: RoseRS_1053
RCA: Rcas_3474
CAU: Caur_0329
CHL: Chy400_0354
CAG: Cagg_3602
HAU: Haur_3302
DRA: DR_1610
DGE: Dgeo_1156
DDR: Deide_13470
DFC: DFI_07080
TRA: Trad_1389
TTH: TT_C1547
TTJ: TTHA1911
TSC: TSC_c01880(hacA)
TAQ: TO73_0094
MRB: Mrub_2728
MJA: MJ_1003
MMP: MMP1480
MMD: GYY_08235
MAE: Maeo_0311
MVO: Mvol_1623
MTH: MTH_1631
MMG: MTBMA_c02160(leuC1)
METC: MTCT_1490
MWO: MWSIV6_0177(hacA)
METE: tca_01581(dmdA_2)
MST: Msp_1100(leuC2)
MSI: Msm_0723
MRU: mru_0384(aksD)
MEB: Abm4_0180(aksD)
MMIL: sm9_2161(aksD)
MEYE: TL18_01065
MOL: YLM1_0055
METH: MBMB1_0289(leuC2)
MFC: BRM9_1986(aksD)
MFI: DSM1535_0178(leuC2)
MCUB: MCBB_0567(hacA)
MFV: Mfer_0965
MKA: MK1208(leuC_2)
MAC: MA_3085(leuC)
MBAR: MSBR2_1672
MBAK: MSBR3_1673
MMA: MM_0409
MMAC: MSMAC_0883
METM: MSMTP_2281
MTHE: MSTHC_0762
MTHR: MSTHT_2523
MHOR: MSHOH_1131
MBU: Mbur_2263(hacA)
MMET: MCMEM_0412
MMH: Mmah_0418
MPY: Mpsy_2821
MTP: Mthe_0788
MCJ: MCON_0444(leuC1)
MHI: Mhar_1525
MHU: Mhun_1800
MLA: Mlab_0233
MBG: BN140_1717(leuC3)
MEMA: MMAB1_2209(leuC)
MPI: Mpet_2489
MBN: Mboo_0599
MPL: Mpal_2143
MPD: MCP_1961
MEZ: Mtc_1559(leuC-2)
RCI: RCIX2646(leuC-2)
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Drevland RM, Jia Y, Palmer DR, Graham DE
  Title
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.
  Journal
J Biol Chem 283:28888-96 (2008)
DOI:10.1074/jbc.M802159200
  Sequence
[mja:MJ_1003]

KEGG   ORTHOLOGY: K16793Help
Entry
K16793                      KO                                     

Name
aksE
Definition
methanogen homoaconitase small subunit [EC:4.2.1.114]
Pathway
ko00300  Lysine biosynthesis
ko00680  Methane metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01130  Biosynthesis of antibiotics
ko01210  2-Oxocarboxylic acid metabolism
ko01230  Biosynthesis of amino acids
Module
M00433  Lysine biosynthesis, 2-oxoglutarate => 2-oxoadipate
M00608  2-Oxocarboxylic acid chain extension, 2-oxoglutarate => 2-oxoadipate => 2-oxopimelate => 2-oxosuberate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00680 Methane metabolism
    K16793  aksE; methanogen homoaconitase small subunit
  09105 Amino acid metabolism
   00300 Lysine biosynthesis
    K16793  aksE; methanogen homoaconitase small subunit
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.114  methanogen homoaconitase
     K16793  aksE; methanogen homoaconitase small subunit
BRITE hierarchy
Other DBs
RN: R03444 R04371 R09720 R10391 R10392 R10393 R10394 R10395 R10396
COG: COG0066
Genes
BOH: AKI39_19115
RRS: RoseRS_1056
RCA: Rcas_3472
CAU: Caur_0330(leuD)
CHL: Chy400_0355
CAG: Cagg_3601
HAU: Haur_3303
DRA: DR_1614(leuD)
DGE: Dgeo_1154
DDR: Deide_13460
DFC: DFI_07070
TRA: Trad_1390
TTH: TT_C1546
TTJ: TTHA1910
TSC: TSC_c01890(hacB)
TAQ: TO73_0095
MRB: Mrub_2727
MJA: MJ_1271
MMP: MMP0381
MAE: Maeo_0652
MVO: Mvol_1671
MTH: MTH_829
MMG: MTBMA_c12270(leuD1)
METC: MTCT_0743
MWO: MWSIV6_1206(hacB)
METE: tca_00796(DmdB_1)
MST: Msp_0374(leuD2)
MSI: Msm_0847
MRU: mru_1689(aksE)
MEB: Abm4_0514(aksE)
MMIL: sm9_1773(aksE)
MEYE: TL18_07930
MOL: YLM1_1157
METH: MBMB1_0696(leuD1)
MFC: BRM9_1499(aksE)
MFI: DSM1535_2193(leuD1)
MCUB: MCBB_0707(hacB)
MFV: Mfer_0205
MKA: MK1206(leuD_2)
CDIV: CPM_1676
MAC: MA_1223(leuC) MA_3751(leuD)
MMA: MM_0645
MMAC: MSMAC_0666
METM: MSMTP_0731
MTHE: MSTHC_0906
MTHR: MSTHT_2374
MHOR: MSHOH_0786
MBU: Mbur_1578
MMET: MCMEM_2089
MMH: Mmah_0127
MPY: Mpsy_2354
MTP: Mthe_0853
MCJ: MCON_1922(leuD1)
MHI: Mhar_2395
MHU: Mhun_1799
MLA: Mlab_0234
MBG: BN140_1716(leuD3)
MPI: Mpet_2490
MBN: Mboo_0600
MPL: Mpal_2144
MPD: MCP_1962
MEZ: Mtc_1558(leuD-2)
RCI: RCIX2645(leuD-2)
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Drevland RM, Jia Y, Palmer DR, Graham DE
  Title
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.
  Journal
J Biol Chem 283:28888-96 (2008)
DOI:10.1074/jbc.M802159200
  Sequence
[mja:MJ_1271]

KEGG   ENZYME: 4.2.1.114Help
Entry
EC 4.2.1.114                Enzyme                                 

Name
methanogen homoaconitase;
methanogen HACN
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming]
Reaction(IUBMB)
(R)-2-hydroxybutane-1,2,4-tricarboxylate = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction) [RN:R09720];
(1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate = (Z)-but-1-ene-1,2,4-tricarboxylate + H2O [RN:R03444];
(1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H2O = (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [RN:R04371]
Reaction(KEGG)
Substrate
(R)-2-hydroxybutane-1,2,4-tricarboxylate [CPD:C01251];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Product
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate [CPD:C05662];
(Z)-but-1-ene-1,2,4-tricarboxylate [CPD:C04002];
H2O [CPD:C00001]
Comment
This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea. Requires a [4Fe-4S] cluster for activity. In contrast to EC 4.2.1.36, homoaconitate hydratase, this enzyme can catalyse both the dehydration of (R)-homocitrate to form cis-homoaconitate and the subsequent hydration reaction that forms homoisocitrate. In addition to cis-homoaconitate, the enzyme can also catalyse the hydration of the physiological substrates dihomocitrate and trihomocitrate as well as the non-physiological substrate tetrahomocitrate. cis-Aconitate and threo-DL-isocitrate cannot act as substrates, and (S)-homocitrate and trans-homoaconitate act as inhibitors of the enzyme.
History
EC 4.2.1.114 created 2009
Pathway
ec00300  Lysine biosynthesis
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
ec01130  Biosynthesis of antibiotics
Orthology
K16792  methanogen homoaconitase large subunit
K16793  methanogen homoaconitase small subunit
Genes
BOH: AKI39_19115
OBJ: EIO64_04565
RRS: RoseRS_1053 RoseRS_1056
RCA: Rcas_3472 Rcas_3474
CAU: Caur_0329 Caur_0330(leuD)
CHL: Chy400_0354 Chy400_0355
CAG: Cagg_3601 Cagg_3602
HAU: Haur_3302 Haur_3303
DRA: DR_1610 DR_1614(leuD)
DGE: Dgeo_1154 Dgeo_1156
TSC: TSC_c01880(hacA) TSC_c01890(hacB)
MMD: GYY_08235
MMG: MTBMA_c02160(leuC1) MTBMA_c12270(leuD1)
MWO: MWSIV6_0177(hacA) MWSIV6_1206(hacB)
METE: tca_00796(DmdB_1) tca_01581(dmdA_2)
MST: Msp_0374(leuD2) Msp_1100(leuC2)
MRU: mru_0384(aksD) mru_1689(aksE)
MEB: Abm4_0180(aksD) Abm4_0514(aksE)
MMIL: sm9_1773(aksE) sm9_2161(aksD)
METH: MBMB1_0289(leuC2) MBMB1_0696(leuD1)
MFC: BRM9_1499(aksE) BRM9_1986(aksD)
MFI: DSM1535_0178(leuC2) DSM1535_2193(leuD1)
MCUB: MCBB_0567(hacA) MCBB_0707(hacB)
MKA: MK1206(leuD_2) MK1208(leuC_2)
CDIV: CPM_1676
MAC: MA_1223(leuC) MA_3085(leuC) MA_3751(leuD)
MBU: Mbur_1578 Mbur_2263(hacA)
MCJ: MCON_0444(leuC1) MCON_1922(leuD1)
MBG: BN140_1716(leuD3) BN140_1717(leuC3)
MEMA: MMAB1_2209(leuC)
MEZ: Mtc_1558(leuD-2) Mtc_1559(leuC-2)
RCI: RCIX2645(leuD-2) RCIX2646(leuC-2)
 » show all
Taxonomy
Reference
1  [PMID:18765671]
  Authors
Drevland RM, Jia Y, Palmer DR, Graham DE
  Title
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.
  Journal
J Biol Chem 283:28888-96 (2008)
DOI:10.1074/jbc.M802159200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.114
IUBMB Enzyme Nomenclature: 4.2.1.114
ExPASy - ENZYME nomenclature database: 4.2.1.114
BRENDA, the Enzyme Database: 4.2.1.114

KEGG   REACTION: R03444Help
Entry
R03444                      Reaction                               

Name
(R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase
Definition
(R)-2-Hydroxybutane-1,2,4-tricarboxylate <=> (Z)-But-1-ene-1,2,4-tricarboxylate + H2O
Equation
Comment
first step of two-step reaction (see R09720, R03444+R04371)
Reaction class
RC00498  C01251_C04002
Enzyme
4.2.1.114       4.2.1.-
Pathway
rn00300  Lysine biosynthesis
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01130  Biosynthesis of antibiotics
rn01210  2-Oxocarboxylic acid metabolism
rn01230  Biosynthesis of amino acids
Module
M00030  Lysine biosynthesis, AAA pathway, 2-oxoglutarate => 2-aminoadipate => lysine
M00433  Lysine biosynthesis, 2-oxoglutarate => 2-oxoadipate
M00608  2-Oxocarboxylic acid chain extension, 2-oxoglutarate => 2-oxoadipate => 2-oxopimelate => 2-oxosuberate
Orthology
K16792  methanogen homoaconitase large subunit [EC:4.2.1.114]
K16793  methanogen homoaconitase small subunit [EC:4.2.1.114]
K17450  homoaconitase [EC:4.2.1.-]
Other DBs
RHEA: 26104

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