KEGG   ORTHOLOGY: K17475
Entry
K17475                      KO                                     

Name
FTMC, CYP5076C
Definition
tryprostatin B 6-hydroxylase [EC:1.14.14.118]
Pathway
ko00404  Staurosporine biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00786  Fumitremorgin alkaloid biosynthesis, tryptophan + proline => fumitremorgin C/A
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K17475  FTMC, CYP5076C; tryprostatin B 6-hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K17475  FTMC, CYP5076C; tryprostatin B 6-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.118  tryprostatin B 6-hydroxylase
     K17475  FTMC, CYP5076C; tryprostatin B 6-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP5076 family
   K17475  FTMC, CYP5076C; tryprostatin B 6-hydroxylase
Other DBs
RN: R10321
COG: COG2124
Genes
AFM: AFUA_8G00190
NFI: NFIA_093700
Reference
  Authors
Kato N, Suzuki H, Takagi H, Asami Y, Kakeya H, Uramoto M, Usui T, Takahashi S, Sugimoto Y, Osada H
  Title
Identification of cytochrome P450s required for fumitremorgin biosynthesis in Aspergillus fumigatus.
  Journal
Chembiochem 10:920-8 (2009)
DOI:10.1002/cbic.200800787
  Sequence

KEGG   ENZYME: 1.14.14.118
Entry
EC 1.14.14.118              Enzyme                                 

Name
tryprostatin B 6-hydroxylase;
ftmC (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
tryprostatin B,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6-hydroxytryprostatin B-forming)
Reaction(IUBMB)
tryprostatin B + [reduced NADPH---hemoprotein reductase] + O2 = 6-hydroxytryprostatin B + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10321]
Reaction(KEGG)
R10321
Substrate
tryprostatin B [CPD:C20512];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
6-hydroxytryprostatin B [CPD:C20513];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. Involved in the biosynthetic pathways of several indole alkaloids such as tryprostatins, fumitremorgins and verruculogen.
History
EC 1.14.14.118 created 2013 as EC 1.14.13.176, transferred 2018 to EC 1.14.14.118
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17475  tryprostatin B 6-hydroxylase
Genes
AFM: AFUA_8G00190
NFI: NFIA_093700
Reference
1  [PMID:19226505]
  Authors
Kato N, Suzuki H, Takagi H, Asami Y, Kakeya H, Uramoto M, Usui T, Takahashi S, Sugimoto Y, Osada H
  Title
Identification of cytochrome P450s required for fumitremorgin biosynthesis in Aspergillus fumigatus.
  Journal
Chembiochem 10:920-8 (2009)
DOI:10.1002/cbic.200800787
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.118
IUBMB Enzyme Nomenclature: 1.14.14.118
ExPASy - ENZYME nomenclature database: 1.14.14.118
BRENDA, the Enzyme Database: 1.14.14.118

KEGG   REACTION: R10321
Entry
R10321                      Reaction                               

Name
tryprostatin B,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6-hydroxytryprostatin B-forming)
Definition
Tryprostatin B + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 6-Hydroxytryprostatin B + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC00490  C20512_C20513
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00786  Fumitremorgin alkaloid biosynthesis, tryptophan + proline => fumitremorgin C/A
Orthology
K17475  tryprostatin B 6-hydroxylase [EC:1.14.14.118]
Other DBs
RHEA: 35958

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