KEGG   ORTHOLOGY: K17476
Entry
K17476                      KO                                     

Name
penM, pntM, CYP161C
Definition
pentalenolactone synthase [EC:1.14.19.8]
Pathway
ko00999  Biosynthesis of various secondary metabolites - part 1
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various secondary metabolites - part 1
    K17476  penM, pntM, CYP161C; pentalenolactone synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K17476  penM, pntM, CYP161C; pentalenolactone synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.8  pentalenolactone synthase
     K17476  penM, pntM, CYP161C; pentalenolactone synthase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, bacteria type
  CYP161 family
   K17476  penM, pntM, CYP161C; pentalenolactone synthase
Other DBs
RN: R10162
COG: COG2124
Genes
SBH: SBI_09672(cyp47)
SCW: TU94_27370
SLE: sle_13230(sle_13230)
SSIA: A7J05_09275
AG: ADO85571(pntM) ADO85587(penM)
Reference
  Authors
Zhu D, Seo MJ, Ikeda H, Cane DE
  Title
Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.
  Journal
J Am Chem Soc 133:2128-31 (2011)
DOI:10.1021/ja111279h
  Sequence

KEGG   ENZYME: 1.14.19.8
Entry
EC 1.14.19.8                Enzyme                                 

Name
pentalenolactone synthase;
penM (gene name);
pntM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
pentalenolactone-reduced-ferredoxin:oxygen oxidoreductase (pentalenolactone forming)
Reaction(IUBMB)
pentalenolactone F + O2 + 2 reduced ferredoxin + 2 H+ = pentalenolactone + 2 oxidized ferredoxin + 2 H2O [RN:R10162]
Reaction(KEGG)
R10162
Substrate
pentalenolactone F [CPD:C20397];
O2 [CPD:C00007];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Product
pentalenolactone [CPD:C20407];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). Isolated from the bacteria Streptomyces exfoliatus and Streptomyces arenae.
History
EC 1.14.19.8 created 2012 as EC 1.3.7.10, transferred 2013 to EC 1.14.19.8
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17476  pentalenolactone synthase
Genes
SBH: SBI_09672(cyp47)
SCW: TU94_27370
SLE: sle_13230(sle_13230)
SSIA: A7J05_09275
Reference
1  [PMID:21284395]
  Authors
Zhu D, Seo MJ, Ikeda H, Cane DE
  Title
Genome mining in streptomyces. Discovery of an unprecedented P450-catalyzed oxidative rearrangement that is the final step in the biosynthesis of pentalenolactone.
  Journal
J Am Chem Soc 133:2128-31 (2011)
DOI:10.1021/ja111279h
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.8
IUBMB Enzyme Nomenclature: 1.14.19.8
ExPASy - ENZYME nomenclature database: 1.14.19.8
BRENDA, the Enzyme Database: 1.14.19.8

KEGG   REACTION: R10162
Entry
R10162                      Reaction                               

Name
pentalenolactone-reduced-ferredoxin:oxygen oxidoreductase (pentalenolactone forming)
Definition
Pentalenolactone F + Oxygen + 2 Reduced ferredoxin + 2 H+ <=> Pentalenolactone + 2 Oxidized ferredoxin + 2 H2O
Equation
C20397 + C00007 + 2 C00138 + 2 C00080 <=> C20407 + 2 C00139 + 2 C00001
Reaction class
RC03132  C20397_C20407
Enzyme
Pathway
rn00999  Biosynthesis of various secondary metabolites - part 1
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Orthology
K17476  pentalenolactone synthase [EC:1.14.19.8]
Other DBs
RHEA: 34578

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