KEGG   ORTHOLOGY: K17645Help
Entry
K17645                      KO                                     

Name
AVNA, CYP60A1
Definition
averantin hydroxylase [EC:1.14.14.116]
Pathway
ko00254  Aflatoxin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00254 Aflatoxin biosynthesis
    K17645  AVNA, CYP60A1; averantin hydroxylase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K17645  AVNA, CYP60A1; averantin hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.116  averantin hydroxylase
     K17645  AVNA, CYP60A1; averantin hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, fungi type
  CYP60 family
   K17645  AVNA, CYP60A1; averantin hydroxylase
BRITE hierarchy
Other DBs
RN: R10317 R10318
Genes
PAN: PODANSg8563
ANI: AN7818.2
AOR: AO090026000021
AFV: AFLA_139260
TaxonomyKoalaUniProt
Reference
PMID:8368836
  Authors
Yabe K, Matsuyama Y, Ando Y, Nakajima H, Hamasaki T
  Title
Stereochemistry during aflatoxin biosynthesis: conversion of norsolorinic acid to averufin.
  Journal
Appl Environ Microbiol 59:2486-92 (1993)

KEGG   ENZYME: 1.14.14.116Help
Entry
EC 1.14.14.116              Enzyme                                 

Name
averantin hydroxylase;
AVN hydroxylase;
avnA (gene name);
CYP60A1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(1'S)-averantin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Reaction(IUBMB)
(1) (1'S)-averantin + [reduced NADPH---hemoprotein reductase] + O2 = (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10317];
(2) (1'S)-averantin + [reduced NADPH---hemoprotein reductase] + O2 = (1'S,5'R)-5'-hydroxyaverantin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R10318]
Reaction(KEGG)
Substrate
(1'S)-averantin [CPD:C20499];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(1'S,5'S)-5'-hydroxyaverantin [CPD:C20500];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
(1'S,5'R)-5'-hydroxyaverantin [CPD:C20501]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the saprophytic mold Aspergillus parasiticus. Involved in aflatoxin biosynthesis. Does not react with (1'R)-averantin.
History
EC 1.14.14.116 created 2013 as EC 1.14.13.174, transferred 2018 to EC 1.14.14.116
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K17645  averantin hydroxylase
Genes
PAN: PODANSg8563
ANI: AN7818.2
AOR: AO090026000021
AFV: AFLA_139260
Taxonomy
Reference
1  [PMID:8368836]
  Authors
Yabe K, Matsuyama Y, Ando Y, Nakajima H, Hamasaki T
  Title
Stereochemistry during aflatoxin biosynthesis: conversion of norsolorinic acid to averufin.
  Journal
Appl Environ Microbiol 59:2486-92 (1993)
Reference
2  [PMID:9097431]
  Authors
Yu J, Chang PK, Cary JW, Bhatnagar D, Cleveland TE
  Title
avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus.
  Journal
Appl Environ Microbiol 63:1349-56 (1997)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.116
IUBMB Enzyme Nomenclature: 1.14.14.116
ExPASy - ENZYME nomenclature database: 1.14.14.116
BRENDA, the Enzyme Database: 1.14.14.116

KEGG   REACTION: R10318Help
Entry
R10318                      Reaction                               

Name
(1'S)-averantin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (5'-hydroxylating)
Definition
(1'S)-Averantin + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> (1'S,5'R)-5'-Hydroxyaverantin + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
Reaction class
RC01710  C20499_C20501
Enzyme
Pathway
rn00254  Aflatoxin biosynthesis
rn01130  Biosynthesis of antibiotics
Orthology
K17645  averantin hydroxylase [EC:1.14.14.116]
Other DBs
RHEA: 35578

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