KEGG   ORTHOLOGY: K17646Help
Entry
K17646                      KO                                     

Name
VBS
Definition
5'-oxoaverantin cyclase / versicolorin B synthase [EC:4.2.1.142 4.2.1.143]
Pathway
ko00254  Aflatoxin biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00254 Aflatoxin biosynthesis
    K17646  VBS; 5'-oxoaverantin cyclase / versicolorin B synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.142  5'-oxoaverantin cyclase
     K17646  VBS; 5'-oxoaverantin cyclase / versicolorin B synthase
    4.2.1.143  versicolorin B synthase
     K17646  VBS; 5'-oxoaverantin cyclase / versicolorin B synthase
BRITE hierarchy
Other DBs
RN: R10402 R10475
GO: 0046572
Genes
PAN: PODANSg8556
ANI: AN7812.2
AOR: AO090026000028
AFV: AFLA_139190
AG: AAC49318(vbs)
TaxonomyKoalaUniProt
Reference
  Authors
Sakuno E, Wen Y, Hatabayashi H, Arai H, Aoki C, Yabe K, Nakajima H
  Title
Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis.
  Journal
Appl Environ Microbiol 71:2999-3006 (2005)
DOI:10.1128/AEM.71.6.2999-3006.2005
  Sequence

KEGG   ENZYME: 4.2.1.143Help
Entry
EC 4.2.1.143                Enzyme                                 

Name
versicolorin B synthase;
versiconal cyclase;
VBS
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
versiconal hydro-lyase (versicolorin-B forming)
Reaction(IUBMB)
versiconal = versicolorin B + H2O [RN:R10402]
Reaction(KEGG)
Substrate
versiconal [CPD:C20507]
Product
versicolorin B [CPD:C20575];
H2O [CPD:C00001]
Comment
Isolated from the aflatoxin-producing mold Aspergillus parasiticus. Involved in aflatoxin biosynthesis.
History
EC 4.2.1.143 created 2013
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K17646  5'-oxoaverantin cyclase / versicolorin B synthase
Genes
PAN: PODANSg8556
ANI: AN7812.2
AOR: AO090026000028
AFV: AFLA_139190
Taxonomy
Reference
1  [PMID:1731640]
  Authors
Lin BK, Anderson JA
  Title
Purification and properties of versiconal cyclase from Aspergillus parasiticus.
  Journal
Arch Biochem Biophys 293:67-70 (1992)
DOI:10.1016/0003-9861(92)90366-5
  Sequence
Reference
2  [PMID:8784203]
  Authors
McGuire SM, Silva JC, Casillas EG, Townsend CA
  Title
Purification and characterization of versicolorin B synthase from Aspergillus parasiticus. Catalysis of the stereodifferentiating cyclization in aflatoxin biosynthesis essential to DNA interaction.
  Journal
Biochemistry 35:11470-86 (1996)
DOI:10.1021/bi960924s
  Sequence
Reference
3  [PMID:8662689]
  Authors
Silva JC, Minto RE, Barry CE 3rd, Holland KA, Townsend CA
  Title
Isolation and characterization of the versicolorin B synthase gene from Aspergillus parasiticus. Expansion of the aflatoxin b1 biosynthetic gene cluster.
  Journal
J Biol Chem 271:13600-8 (1996)
DOI:10.1074/jbc.271.23.13600
  Sequence
Reference
4  [PMID:8995367]
  Authors
Silva JC, Townsend CA
  Title
Heterologous expression, isolation, and characterization of versicolorin B synthase from Aspergillus parasiticus. A key enzyme in the aflatoxin B1 biosynthetic pathway.
  Journal
J Biol Chem 272:804-13 (1997)
DOI:10.1074/jbc.272.2.804
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.143
IUBMB Enzyme Nomenclature: 4.2.1.143
ExPASy - ENZYME nomenclature database: 4.2.1.143
BRENDA, the Enzyme Database: 4.2.1.143

KEGG   REACTION: R10402Help
Entry
R10402                      Reaction                               

Name
versiconal hydro-lyase (versicolorin-B forming)
Definition
Versiconal <=> Versicolorin B + H2O
Equation
Reaction class
RC03146  C20507_C20575
Enzyme
Pathway
rn00254  Aflatoxin biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Orthology
K17646  5'-oxoaverantin cyclase / versicolorin B synthase [EC:4.2.1.142 4.2.1.143]
Other DBs
RHEA: 33862

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