KEGG   ORTHOLOGY: K17650
Entry
K17650                      KO                                     

Name
OMTA
Definition
sterigmatocystin 8-O-methyltransferase [EC:2.1.1.110]
Pathway
ko00254  Aflatoxin biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00254 Aflatoxin biosynthesis
    K17650  OMTA; sterigmatocystin 8-O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.110  sterigmatocystin 8-O-methyltransferase
     K17650  OMTA; sterigmatocystin 8-O-methyltransferase
Other DBs
RN: R03111 R10307
GO: 0047146
Genes
AFM: AFUA_5G02640
ACT: ACLA_002520
NFI: NFIA_039440
AOR: AO090026000026
AFV: AFLA_139210
AG: AAA32697(OMTA)
Reference
PMID:7557460
  Authors
Yu J, Chang PK, Payne GA, Cary JW, Bhatnagar D, Cleveland TE
  Title
Comparison of the omtA genes encoding O-methyltransferases involved in aflatoxin biosynthesis from Aspergillus parasiticus and A. flavus.
  Journal
Gene 163:121-5 (1995)
DOI:10.1016/0378-1119(95)00397-O
  Sequence

KEGG   ENZYME: 2.1.1.110
Entry
EC 2.1.1.110                Enzyme                                 

Name
sterigmatocystin 8-O-methyltransferase;
sterigmatocystin methyltransferase;
O-methyltransferase II;
sterigmatocystin 7-O-methyltransferase (incorrect);
S-adenosyl-L-methionine:sterigmatocystin 7-O-methyltransferase (incorrect);
OmtA
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:sterigmatocystin 8-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + sterigmatocystin = S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin [RN:R03111];
(2) S-adenosyl-L-methionine + dihydrosterigmatocystin = S-adenosyl-L-homocysteine + 8-O-methyldihydrosterigmatocystin [RN:R10307]
Reaction(KEGG)
R03111 R10307
Substrate
S-adenosyl-L-methionine [CPD:C00019];
sterigmatocystin [CPD:C00961];
dihydrosterigmatocystin [CPD:C20445]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
8-O-methylsterigmatocystin [CPD:C03686];
8-O-methyldihydrosterigmatocystin [CPD:C03944]
Comment
Dihydrosterigmatocystin can also act as acceptor. Involved in the biosynthesis of aflatoxins in fungi.
History
EC 2.1.1.110 created 1992, modified 2005, modified 2013
Pathway
ec00254  Aflatoxin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17650  sterigmatocystin 8-O-methyltransferase
Genes
AFM: AFUA_5G02640
ACT: ACLA_002520
NFI: NFIA_039440
AOR: AO090026000026
AFV: AFLA_139210
Reference
1  [PMID:3111363]
  Authors
Bhatnagar D, McCormick SP, Lee LS, Hill RA.
  Title
Identification of O-methylsterigmatocystin as an aflatoxin B1 and G1 precursor in Aspergillus parasiticus.
  Journal
Appl Environ Microbiol 53:1028-33 (1987)
DOI:10.1128/AEM.53.5.1028-1033.1987
Reference
2  [PMID:2802602]
  Authors
Yabe K, Ando Y, Hashimoto J, Hamasaki T.
  Title
Two distinct O-methyltransferases in aflatoxin biosynthesis.
  Journal
Appl Environ Microbiol 55:2172-7 (1989)
DOI:10.1128/AEM.55.9.2172-2177.1989
Reference
3  [PMID:8285664]
  Authors
Yu J, Cary JW, Bhatnagar D, Cleveland TE, Keller NP, Chu FS.
  Title
Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis.
  Journal
Appl Environ Microbiol 59:3564-71 (1993)
DOI:10.1128/AEM.59.11.3564-3571.1993
  Sequence
Reference
4  [PMID:12406770]
  Authors
Lee LW, Chiou CH, Linz JE
  Title
Function of native OmtA in vivo and expression and distribution of this protein in colonies of Aspergillus parasiticus.
  Journal
Appl Environ Microbiol 68:5718-27 (2002)
DOI:10.1128/AEM.68.11.5718-5727.2002
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.110
IUBMB Enzyme Nomenclature: 2.1.1.110
ExPASy - ENZYME nomenclature database: 2.1.1.110
BRENDA, the Enzyme Database: 2.1.1.110
CAS: 116958-29-3

KEGG   REACTION: R10307
Entry
R10307                      Reaction                               

Name
S-adenosyl-L-methionine:dihydrosterigmatocystin 8-O-methyltransferase
Definition
S-Adenosyl-L-methionine + Dihydrosterigmatocystin <=> S-Adenosyl-L-homocysteine + Dihydro-O-methylsterigmatocystin
Equation
Reaction class
RC00003  C00019_C00021
RC00392  C03944_C20445
Enzyme
Pathway
rn00254  Aflatoxin biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K17650  sterigmatocystin 8-O-methyltransferase [EC:2.1.1.110]
Other DBs
RHEA: 35770

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