KEGG   ORTHOLOGY: K17825Help
Entry
K17825                      KO                                     

Name
FTMF
Definition
verruculogen synthase [EC:1.14.11.38]
Pathway
ko00404  Staurosporine biosynthesis
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00786  Fumitremorgin alkaloid biosynthesis, tryptophan + proline => fumitremorgin C/A
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00404 Staurosporine biosynthesis
    K17825  FTMF; verruculogen synthase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.38  verruculogen synthase
     K17825  FTMF; verruculogen synthase
BRITE hierarchy
Other DBs
RN: R10445
Genes
AFM: AFUA_8G00230
NFI: NFIA_045530 NFIA_093740
URE: UREG_04217 UREG_07661
TaxonomyKoalaUniProt
Reference
  Authors
Steffan N, Grundmann A, Afiyatullov S, Ruan H, Li SM
  Title
FtmOx1, a non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus.
  Journal
Org Biomol Chem 7:4082-7 (2009)
DOI:10.1039/b908392h
  Sequence

KEGG   ENZYME: 1.14.11.38Help
Entry
EC 1.14.11.38               Enzyme                                 

Name
verruculogen synthase;
fmtF (gene name);
FmtOx1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
fumitremorgin B,2-oxoglutarate:oxygen oxidoreductase (verruculogen-forming)
Reaction(IUBMB)
fumitremorgin B + 2-oxoglutarate + 2 O2 + reduced acceptor = verruculogen + succinate + CO2 + H2O + acceptor [RN:R10445]
Reaction(KEGG)
Substrate
fumitremorgin B [CPD:C20630];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007];
reduced acceptor [CPD:C00030]
Product
verruculogen [CPD:C20045];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001];
acceptor [CPD:C00028]
Comment
Requires Fe2+ and ascorbate. Found in the fungus Aspergillus fumigatus. Both atoms of a dioxygen molecule are incorporated into verruculogen [1,2]. Involved in the biosynthetic pathways of several indole alkaloids such as fumitremorgin A.
History
EC 1.14.11.38 created 2013
Pathway
ec00404  Staurosporine biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K17825  verruculogen synthase
Genes
AFM: AFUA_8G00230
NFI: NFIA_045530 NFIA_093740
URE: UREG_04217 UREG_07661
Taxonomy
Reference
1  [PMID:19763315]
  Authors
Steffan N, Grundmann A, Afiyatullov S, Ruan H, Li SM
  Title
FtmOx1, a non-heme Fe(II) and alpha-ketoglutarate-dependent dioxygenase, catalyses the endoperoxide formation of verruculogen in Aspergillus fumigatus.
  Journal
Org Biomol Chem 7:4082-7 (2009)
DOI:10.1039/b908392h
  Sequence
Reference
2  [PMID:21404415]
  Authors
Kato N, Suzuki H, Takagi H, Uramoto M, Takahashi S, Osada H
  Title
Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigatus.
  Journal
Chembiochem 12:711-4 (2011)
DOI:10.1002/cbic.201000562
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.38
IUBMB Enzyme Nomenclature: 1.14.11.38
ExPASy - ENZYME nomenclature database: 1.14.11.38
BRENDA, the Enzyme Database: 1.14.11.38

KEGG   REACTION: R10445Help
Entry
R10445                      Reaction                               

Name
fumitremorgin B,2-oxoglutarate:oxygen oxidoreductase (verruculogen-forming)
Definition
Fumitremorgin B + 2-Oxoglutarate + 2 Oxygen + Reduced acceptor <=> Verruculogen + Succinate + CO2 + H2O + Acceptor
Equation
Reaction class
RC03169  C20045_C20630
Enzyme
Pathway
rn00404  Staurosporine biosynthesis
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00786  Fumitremorgin alkaloid biosynthesis, tryptophan + proline => fumitremorgin C/A
Orthology
K17825  verruculogen synthase [EC:1.14.11.38]
Other DBs
RHEA: 35978

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