KEGG   ORTHOLOGY: K17835
Entry
K17835                      KO                                     

Name
griH
Definition
3-amino-4-hydroxybenzoic acid synthase [EC:4.1.99.20]
Pathway
ko00997  Biosynthesis of various secondary metabolites - part 3
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Module
M00905  Grixazone biosynthesis, aspartate 4-semialdehyde => grixazone B
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00997 Biosynthesis of various secondary metabolites - part 3
    K17835  griH; 3-amino-4-hydroxybenzoic acid synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.1  Carbon-carbon lyases
   4.1.99  Other carbon-carbon lyases
    4.1.99.20  3-amino-4-hydroxybenzoate synthase
     K17835  griH; 3-amino-4-hydroxybenzoic acid synthase
Other DBs
RN: R10469
COG: COG1465
Genes
MII: BTJ40_01850
CRZ: D1345_21920
BUR: Bcep18194_C6863
BCEP: APZ15_37510
BSTL: BBJ41_35550
BUZ: AYM40_17235
PBH: AAW51_0199
SUR: STAUR_3233 STAUR_6099
CFUS: CYFUS_007180
CCRO: CMC5_017700(aroB)
CCE: Ccel_2374
HMO: HM1_1788(aroB)
GOM: D7316_02314(griH)
SGR: SGR_4248(griH)
SBH: SBI_01605
SVE: SVEN_5125
SLD: T261_2096
SPRI: SPRI_1930
SNR: SNOUR_07210(griH)
SFK: KY5_6733
MPH: MLP_27050
SEN: SACE_5077
SESP: BN6_21370(griH1) BN6_63890(griH2)
KAL: KALB_2250
ACTN: L083_5378
 » show all
Reference
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J Biol Chem 281:36944-51 (2006)
DOI:10.1074/jbc.M608103200
  Sequence
[sgr:SGR_4248]

KEGG   ENZYME: 4.1.99.20
Entry
EC 4.1.99.20                Enzyme                                 

Name
3-amino-4-hydroxybenzoate synthase;
3,4-AHBA synthase;
griH (gene name)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
Sysname
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate hydro-lyase (cyclizing, 3-amino-4-hydroxybenzoate-forming)
Reaction(IUBMB)
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate = 3-amino-4-hydroxybenzoate + phosphate + 2 H2O [RN:R10469]
Reaction(KEGG)
R10469
Substrate
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate [CPD:C20644]
Product
3-amino-4-hydroxybenzoate [CPD:C12115];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
Requires Mn2+ for maximum activity. The reaction is suggested to take place in several steps. Schiff base formation, double bond migration and dephosphorylation followed by ring opening and closing to form a pyrrolidine ring, and finally dehydration to form the product 3-amino-4-hydroxybenzoate. In the bacterium Streptomyces griseus the enzyme is involved in biosynthesis of grixazone, a yellow pigment that contains a phenoxazinone chromophore.
History
EC 4.1.99.20 created 2013, modified 2016
Pathway
ec00997  Biosynthesis of various secondary metabolites - part 3
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K17835  3-amino-4-hydroxybenzoic acid synthase
Genes
MII: BTJ40_01850
CRZ: D1345_21920
BUR: Bcep18194_C6863
BCEP: APZ15_37510
BSTL: BBJ41_35550
BUZ: AYM40_17235
PBH: AAW51_0199
SUR: STAUR_3233 STAUR_6099
CFUS: CYFUS_007180
CCRO: CMC5_017700(aroB)
CCE: Ccel_2374
HMO: HM1_1788(aroB)
GOM: D7316_02314(griH)
SGR: SGR_4248(griH)
SBH: SBI_01605
SVE: SVEN_5125
SLD: T261_2096
SPRI: SPRI_1930
SNR: SNOUR_07210(griH)
SFK: KY5_6733
MPH: MLP_27050
SEN: SACE_5077
SESP: BN6_21370(griH1) BN6_63890(griH2)
KAL: KALB_2250
ACTN: L083_5378
 » show all
Reference
1  [PMID:17003031]
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S.
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J Biol Chem 281:36944-51 (2006)
DOI:10.1074/jbc.M608103200
  Sequence
[sgr:SGR_4248]
Other DBs
ExplorEnz - The Enzyme Database: 4.1.99.20
IUBMB Enzyme Nomenclature: 4.1.99.20
ExPASy - ENZYME nomenclature database: 4.1.99.20
BRENDA, the Enzyme Database: 4.1.99.20

KEGG   REACTION: R10469
Entry
R10469                      Reaction                               

Name
2-amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate hydro-lyase (cyclizing, 3-amino-4-hydroxybenzoate-forming)
Definition
2-Amino-4,5-dihydroxy-6-oxo-7-(phosphooxy)heptanoate <=> 3-Amino-4-hydroxybenzoate + Orthophosphate + 2 H2O
Equation
Reaction class
RC03177  C12115_C20644
Enzyme
Pathway
rn00997  Biosynthesis of various secondary metabolites - part 3
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Module
M00905  Grixazone biosynthesis, aspartate 4-semialdehyde => grixazone B
Orthology
K17835  3-amino-4-hydroxybenzoic acid synthase [EC:4.1.99.20]
Other DBs
RHEA: 26320

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