KEGG   ORTHOLOGY: K17877
Entry
K17877                      KO                                     

Name
NIT-6
Definition
nitrite reductase (NAD(P)H) [EC:1.7.1.4]
Pathway
ko00910  Nitrogen metabolism
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
Module
M00531  Assimilatory nitrate reduction, nitrate => ammonia
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09102 Energy metabolism
   00910 Nitrogen metabolism
    K17877  NIT-6; nitrite reductase (NAD(P)H)
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.7  Acting on other nitrogenous compounds as donors
   1.7.1  With NAD+ or NADP+ as acceptor
    1.7.1.4  nitrite reductase [NAD(P)H]
     K17877  NIT-6; nitrite reductase (NAD(P)H)
Other DBs
RN: R00787 R00789
GO: 0008942
Genes
QSU: 111999790 112036833
DHA: DEHA2G21890g
NCR: NCU04720(nit-6)
NTE: NEUTE1DRAFT87025(NEUTE1DRAFT_87025)
SMP: SMAC_03842
PAN: PODANSg3187
TTT: THITE_2110175
MTM: MYCTH_2306799
CTHR: CTHT_0041190
MGR: MGG_00634
SSCK: SPSK_02946
MAW: MAC_03493
MAJ: MAA_02214
BFU: BCIN_01g05790(BcniiA)
ANI: AN1007.2
ANG: ANI_1_804074(An08g05640)
PTE: PTT_08193
 » show all
Reference
PMID:8096840
  Authors
Exley GE, Colandene JD, Garrett RH
  Title
Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.
  Journal
J Bacteriol 175:2379-92 (1993)
DOI:10.1128/JB.175.8.2379-2392.1993
  Sequence
[ncr:NCU04720]

KEGG   ENZYME: 1.7.1.4
Entry
EC 1.7.1.4                  Enzyme                                 

Name
nitrite reductase [NAD(P)H];
nitrite reductase (reduced nicotinamide adenine dinucleotide (phosphate));
assimilatory nitrite reductase (ambiguous);
nitrite reductase [NAD(P)H2];
NAD(P)H2:nitrite oxidoreductase;
nit-6 (gene name)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
Sysname
ammonia:NAD(P)+ oxidoreductase
Reaction(IUBMB)
NH3 + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H + 5 H+ [RN:R00787 R00789]
Reaction(KEGG)
R00787 R00789
Substrate
NH3 [CPD:C00014];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
nitrite [CPD:C00088];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
An iron-sulfur flavoprotein (FAD) containing siroheme. The enzymes from the fungi Neurospora crassa [1], Emericella nidulans [2] and Candida nitratophila [4] can use either NADPH or NADH as electron donor. cf. EC 1.7.1.15, nitrite reductase (NADH).
History
EC 1.7.1.4 created 1961 as EC 1.6.6.4, transferred 2002 to EC 1.7.1.4, modified 2013
Pathway
ec00910  Nitrogen metabolism
Orthology
K17877  nitrite reductase (NAD(P)H)
Genes
QSU: 111999790 112036833
DHA: DEHA2G21890g
NCR: NCU04720(nit-6)
NTE: NEUTE1DRAFT87025(NEUTE1DRAFT_87025)
SMP: SMAC_03842
PAN: PODANSg3187
TTT: THITE_2110175
MTM: MYCTH_2306799
CTHR: CTHT_0041190
MGR: MGG_00634
SSCK: SPSK_02946
MAW: MAC_03493
MAJ: MAA_02214
BFU: BCIN_01g05790(BcniiA)
ANI: AN1007.2
ANG: ANI_1_804074(An08g05640)
PTE: PTT_08193
 » show all
Reference
1  [PMID:14426899]
  Authors
NICHOLAS DJ, MEDINA A, JONES OT
  Title
A nitrite reductase from Neurospora crassa.
  Journal
Biochim Biophys Acta 37:468-76 (1960)
DOI:10.1016/0006-3002(60)90503-5
Reference
2  [PMID:4382427]
  Authors
Pateman JA, Rever BM, Cove DJ
  Title
Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans.
  Journal
Biochem J 104:103-11 (1967)
DOI:10.1042/bj1040103
Reference
3
  Authors
Rivas, J., Guerrero, M. G., Paneque, A. and Losada, M.
  Title
Characterization of the nitrate-reducing system of the yeast Torulopsis nitratophila.
  Journal
Plant Sci Lett 1:105-113 (1973)
Reference
4  [PMID:4154942]
  Authors
Lafferty MA, Garrett RH
  Title
Purification and properties of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J Biol Chem 249:7555-67 (1974)
Reference
5  [PMID:126995]
  Authors
Vega JM, Garrett RH
  Title
Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J Biol Chem 250:7980-9 (1975)
Reference
6  [PMID:150863]
  Authors
Greenbaum P, Prodouz KN, Garrett RH
  Title
Preparation and some properties of homogeneous Neurospora crassa assimilatory NADPH-nitrite reductase.
  Journal
Biochim Biophys Acta 526:52-64 (1978)
DOI:10.1016/0005-2744(78)90289-9
Reference
7  [PMID:6457037]
  Authors
Prodouz KN, Garrett RH
  Title
Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure.
  Journal
J Biol Chem 256:9711-7 (1981)
Reference
8  [PMID:8096840]
  Authors
Exley GE, Colandene JD, Garrett RH
  Title
Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.
  Journal
J Bacteriol 175:2379-92 (1993)
DOI:10.1128/JB.175.8.2379-2392.1993
  Sequence
[ncr:NCU04720]
Reference
9  [PMID:8798648]
  Authors
Colandene JD, Garrett RH.
  Title
Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa.
  Journal
J Biol Chem 271:24096-104 (1996)
DOI:10.1074/jbc.271.39.24096
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.4
IUBMB Enzyme Nomenclature: 1.7.1.4
ExPASy - ENZYME nomenclature database: 1.7.1.4
UM-BBD (Biocatalysis/Biodegradation Database): 1.7.1.4
BRENDA, the Enzyme Database: 1.7.1.4
CAS: 9029-29-2

KEGG   REACTION: R00787
Entry
R00787                      Reaction                               

Name
ammonia:NAD+ oxidoreductase
Definition
Ammonia + 3 NAD+ + 2 H2O <=> Nitrite + 3 NADH + 3 H+
Equation
C00014 + 3 C00003 + 2 C00001 <=> C00088 + 3 C00004 + 3 C00080
Comment
NADPH (see R00789)
NH3 + H2O <=> NH4OH
Reaction class
RC00001  C00003_C00004
RC00176  C00014_C00088
Enzyme
1.7.1.4         1.7.1.15
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00530  Dissimilatory nitrate reduction, nitrate => ammonia
M00531  Assimilatory nitrate reduction, nitrate => ammonia
Orthology
K00362  nitrite reductase (NADH) large subunit [EC:1.7.1.15]
K00363  nitrite reductase (NADH) small subunit [EC:1.7.1.15]
K17877  nitrite reductase (NAD(P)H) [EC:1.7.1.4]
Other DBs
RHEA: 24631

KEGG   REACTION: R00789
Entry
R00789                      Reaction                               

Name
ammonia:NADP+ oxidoreductase
Definition
Ammonia + 3 NADP+ + 2 H2O <=> Nitrite + 3 NADPH + 3 H+
Equation
C00014 + 3 C00006 + 2 C00001 <=> C00088 + 3 C00005 + 3 C00080
Comment
NADH (see R00787)
NH3 + H2O <=> NH4OH
Reaction class
RC00001  C00005_C00006
RC00176  C00014_C00088
Enzyme
Pathway
rn00910  Nitrogen metabolism
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
Module
M00530  Dissimilatory nitrate reduction, nitrate => ammonia
M00531  Assimilatory nitrate reduction, nitrate => ammonia
Orthology
K17877  nitrite reductase (NAD(P)H) [EC:1.7.1.4]
Other DBs
RHEA: 24635

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