KEGG   ORTHOLOGY: K18056Help
Entry
K18056                      KO                                     

Name
pntH, ptlH
Definition
1-deoxypentalenic acid 11beta-hydroxylase [EC:1.14.11.35]
Pathway
ko00999  Biosynthesis of various secondary metabolites - part 1
ko01100  Metabolic pathways
ko01130  Biosynthesis of antibiotics
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00999 Biosynthesis of various secondary metabolites - part 1
    K18056  pntH, ptlH; 1-deoxypentalenic acid 11beta-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.11  With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
    1.14.11.35  1-deoxypentalenic acid 11beta-hydroxylase
     K18056  pntH, ptlH; 1-deoxypentalenic acid 11beta-hydroxylase
BRITE hierarchy
Other DBs
RN: R10157
Genes
SMA: SAVERM_2991(ptlH)
SBH: SBI_09673
SCI: B446_29665
SCW: TU94_27375
SLE: sle_13220(sle_13220)
SSIA: A7J05_09270
TaxonomyKoalaUniProt
Reference
  Authors
You Z, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:6566-7 (2006)
DOI:10.1021/ja061469i
  Sequence

KEGG   ENZYME: 1.14.11.35Help
Entry
EC 1.14.11.35               Enzyme                                 

Name
1-deoxypentalenic acid 11beta-hydroxylase;
ptlH (gene name);
sav2991 (gene name);
pntH (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
1-deoxypentalenate + 2-oxoglutarate + O2 = 1-deoxy-11beta-hydroxypentalenate + succinate + CO2 [RN:R10157]
Reaction(KEGG)
Substrate
1-deoxypentalenate [CPD:C20404];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
1-deoxy-11beta-hydroxypentalenate [CPD:C20403];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
The enzyme requires Fe(II) and ascorbate. Isolated from the bacterium Streptomyces avermitilis. Part of the pathway for pentalenolactone biosynthesis.
History
EC 1.14.11.35 created 2012
Pathway
ec00999  Biosynthesis of various secondary metabolites - part 1
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K18056  1-deoxypentalenic acid 11beta-hydroxylase
Genes
SMA: SAVERM_2991(ptlH)
SBH: SBI_09673
SCI: B446_29665
SCW: TU94_27375
SLE: sle_13220(sle_13220)
SSIA: A7J05_09270
Taxonomy
Reference
1  [PMID:16704250]
  Authors
You Z, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlH, a non-heme iron dioxygenase of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:6566-7 (2006)
DOI:10.1021/ja061469i
  Sequence
Reference
2  [PMID:17942405]
  Authors
You Z, Omura S, Ikeda H, Cane DE, Jogl G
  Title
Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis.
  Journal
J Biol Chem 282:36552-60 (2007)
DOI:10.1074/jbc.M706358200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.11.35
IUBMB Enzyme Nomenclature: 1.14.11.35
ExPASy - ENZYME nomenclature database: 1.14.11.35
BRENDA, the Enzyme Database: 1.14.11.35

KEGG   REACTION: R10157Help
Entry
R10157                      Reaction                               

Name
1-deoxypentalenic acid,2-oxoglutarate:oxygen oxidoreductase
Definition
1-Deoxypentalenate + 2-Oxoglutarate + Oxygen <=> 1-Deoxy-11beta-hydroxypentalenate + Succinate + CO2
Equation
Reaction class
RC00661  C20403_C20404
Enzyme
Pathway
rn00999  Biosynthesis of various secondary metabolites - part 1
rn01100  Metabolic pathways
rn01130  Biosynthesis of antibiotics
Module
M00819  Pentalenolactone biosynthesis, farnesyl-PP => pentalenolactone
Orthology
K18056  1-deoxypentalenic acid 11beta-hydroxylase [EC:1.14.11.35]
Other DBs
RHEA: 34622

DBGET integrated database retrieval system