KEGG   ORTHOLOGY: K18074Help
Entry
K18074                      KO                                     

Name
tphA2
Definition
terephthalate 1,2-dioxygenase oxygenase component alpha subunit [EC:1.14.12.15]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00624  Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00624 Polycyclic aromatic hydrocarbon degradation
    K18074  tphA2; terephthalate 1,2-dioxygenase oxygenase component alpha subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.15  terephthalate 1,2-dioxygenase
     K18074  tphA2; terephthalate 1,2-dioxygenase oxygenase component alpha subunit
BRITE hierarchy
Other DBs
RN: R05148
GO: 0018628
Genes
VPD: VAPA_2c09660
CTT: CtCNB1_1493
CTES: O987_22455
RTA: Rta_16750
HYB: Q5W_19735
TaxonomyKoalaUniProt
Reference
PMID:7961417
  Authors
Schlafli HR, Weiss MA, Leisinger T, Cook AM
  Title
Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component.
  Journal
J Bacteriol 176:6644-52 (1994)
DOI:10.1128/JB.176.21.6644-6652.1994
  Sequence
Reference
  Authors
Fukuhara Y, Kasai D, Katayama Y, Fukuda M, Masai E
  Title
Enzymatic properties of terephthalate 1,2-dioxygenase of Comamonas sp. strain E6.
  Journal
Biosci Biotechnol Biochem 72:2335-41 (2008)
DOI:10.1271/bbb.80236

KEGG   ORTHOLOGY: K18075Help
Entry
K18075                      KO                                     

Name
tphA3
Definition
terephthalate 1,2-dioxygenase oxygenase component beta subunit [EC:1.14.12.15]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00624  Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00624 Polycyclic aromatic hydrocarbon degradation
    K18075  tphA3; terephthalate 1,2-dioxygenase oxygenase component beta subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.15  terephthalate 1,2-dioxygenase
     K18075  tphA3; terephthalate 1,2-dioxygenase oxygenase component beta subunit
BRITE hierarchy
Other DBs
RN: R05148
GO: 0018628
Genes
VPD: VAPA_2c09670
CTT: CtCNB1_1494
CTES: O987_22460
RTA: Rta_16740
HYB: Q5W_19730
TaxonomyKoalaUniProt
Reference
PMID:7961417
  Authors
Schlafli HR, Weiss MA, Leisinger T, Cook AM
  Title
Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component.
  Journal
J Bacteriol 176:6644-52 (1994)
DOI:10.1128/JB.176.21.6644-6652.1994
  Sequence
Reference
  Authors
Fukuhara Y, Kasai D, Katayama Y, Fukuda M, Masai E
  Title
Enzymatic properties of terephthalate 1,2-dioxygenase of Comamonas sp. strain E6.
  Journal
Biosci Biotechnol Biochem 72:2335-41 (2008)
DOI:10.1271/bbb.80236

KEGG   ORTHOLOGY: K18077Help
Entry
K18077                      KO                                     

Name
tphA1
Definition
terephthalate 1,2-dioxygenase reductase component [EC:1.18.1.-]
Pathway
ko00624  Polycyclic aromatic hydrocarbon degradation
ko01100  Metabolic pathways
ko01120  Microbial metabolism in diverse environments
ko01220  Degradation of aromatic compounds
Module
M00624  Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00624 Polycyclic aromatic hydrocarbon degradation
    K18077  tphA1; terephthalate 1,2-dioxygenase reductase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.-  
     K18077  tphA1; terephthalate 1,2-dioxygenase reductase component
BRITE hierarchy
Other DBs
RN: R05148
Genes
VPD: VAPA_2c09690
CTT: CtCNB1_1496
CTES: O987_22470
RTA: Rta_16720
HYB: Q5W_19720
TaxonomyKoalaUniProt
Reference
  Authors
Fukuhara Y, Kasai D, Katayama Y, Fukuda M, Masai E
  Title
Enzymatic properties of terephthalate 1,2-dioxygenase of Comamonas sp. strain E6.
  Journal
Biosci Biotechnol Biochem 72:2335-41 (2008)
DOI:10.1271/bbb.80236

KEGG   ENZYME: 1.14.12.15Help
Entry
EC 1.14.12.15               Enzyme                                 

Name
terephthalate 1,2-dioxygenase;
benzene-1,4-dicarboxylate 1,2-dioxygenase;
1,4-dicarboxybenzoate 1,2-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
benzene-1,4-dicarboxylate,NADH:oxygen oxidoreductase (1,2-hydroxylating)
Reaction(IUBMB)
terephthalate + NADH + H+ + O2 = (1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate + NAD+ [RN:R05148]
Reaction(KEGG)
Substrate
terephthalate [CPD:C06337];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
(1R,6S)-dihydroxycyclohexa-2,4-diene-1,4-dicarboxylate [CPD:C06318];
NAD+ [CPD:C00003]
Comment
Has been shown to contain a Rieske [2Fe-2S] cluster
History
EC 1.14.12.15 created 1999
Pathway
ec00624  Polycyclic aromatic hydrocarbon degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K18074  terephthalate 1,2-dioxygenase oxygenase component alpha subunit
K18075  terephthalate 1,2-dioxygenase oxygenase component beta subunit
Genes
VPD: VAPA_2c09660 VAPA_2c09670
CTT: CtCNB1_1493 CtCNB1_1494
CTES: O987_22455 O987_22460
RTA: Rta_16740 Rta_16750
HYB: Q5W_19730 Q5W_19735
Taxonomy
Reference
1  [PMID:7961417]
  Authors
Schlafli HR, Weiss MA, Leisinger T, Cook AM.
  Title
Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: purification and some properties of the oxygenase component.
  Journal
J Bacteriol 176:6644-52 (1994)
DOI:10.1128/JB.176.21.6644-6652.1994
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.12.15
IUBMB Enzyme Nomenclature: 1.14.12.15
ExPASy - ENZYME nomenclature database: 1.14.12.15
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.12.15
BRENDA, the Enzyme Database: 1.14.12.15
CAS: 162032-76-0

KEGG   REACTION: R05148Help
Entry
R05148                      Reaction                               

Name
benzene-1,4-dicarboxylate, NADH:oxygen oxidoreductase (1,2-hydroxylating)
Definition
Terephthalate + Oxygen + NADH + H+ <=> (3S,4R)-3,4-Dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+
Equation
Reaction class
RC00001  C00003_C00004
RC00270  C06318_C06337
Enzyme
Pathway
rn00624  Polycyclic aromatic hydrocarbon degradation
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01220  Degradation of aromatic compounds
Module
M00624  Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Orthology
K18074  terephthalate 1,2-dioxygenase oxygenase component alpha subunit [EC:1.14.12.15]
K18075  terephthalate 1,2-dioxygenase oxygenase component beta subunit [EC:1.14.12.15]
K18077  terephthalate 1,2-dioxygenase reductase component [EC:1.18.1.-]
Other DBs
RHEA: 10315

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