KEGG   ORTHOLOGY: K18541Help
Entry
K18541                      KO                                     

Name
mdlY
Definition
mandelamide amidase [EC:3.5.1.86]
Pathway
ko00627  Aminobenzoate degradation
ko01120  Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09111 Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K18541  mdlY; mandelamide amidase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.1  In linear amides
    3.5.1.86  mandelamide amidase
     K18541  mdlY; mandelamide amidase
BRITE hierarchy
Other DBs
RN: R05783
COG: COG0154
GO: 0050537
Genes
PPUN: PP4_48060(gatA)
PSIL: PMA3_26265
TaxonomyKoalaUniProt
Reference
  Authors
Gopalakrishna KN, Stewart BH, Kneen MM, Andricopulo AD, Kenyon GL, McLeish MJ
  Title
Mandelamide hydrolase from Pseudomonas putida: characterization of a new member of the amidase signature family.
  Journal
Biochemistry 43:7725-35 (2004)
DOI:10.1021/bi049907q
  Sequence
[ppun:PP4_48060]

KEGG   ENZYME: 3.5.1.86Help
Entry
EC 3.5.1.86                 Enzyme                                 

Name
mandelamide amidase;
Pseudomonas mandelamide hydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
mandelamide hydrolase
Reaction(IUBMB)
(R)-mandelamide + H2O = (R)-mandelate + NH3 [RN:R05783]
Reaction(KEGG)
Substrate
(R)-mandelamide [CPD:C07301];
H2O [CPD:C00001]
Product
(R)-mandelate [CPD:C01983];
NH3 [CPD:C00014]
History
EC 3.5.1.86 created 2000
Pathway
ec00627  Aminobenzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K18541  mandelamide amidase
Genes
PPUN: PP4_48060(gatA)
PSIL: PMA3_26265
Taxonomy
Reference
1  [PMID:1660699]
  Authors
Yamamoto K, Oishi K, Fujimatsu I, Komatsu K.
  Title
Production of R-(-)-mandelic acid from mandelonitrile by Alcaligenes faecalis ATCC 8750.
  Journal
Appl Environ Microbiol 57:3028-32 (1991)
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.86
IUBMB Enzyme Nomenclature: 3.5.1.86
ExPASy - ENZYME nomenclature database: 3.5.1.86
BRENDA, the Enzyme Database: 3.5.1.86

KEGG   REACTION: R05783Help
Entry
R05783                      Reaction                               

Name
mandelamide hydrolase
Definition
(R)-Mandelamide + H2O <=> (R)-Mandelate + Ammonia
Equation
Reaction class
RC00358  C01983_C07301
Enzyme
Pathway
rn00627  Aminobenzoate degradation
rn01120  Microbial metabolism in diverse environments
Orthology
K18541  mandelamide amidase [EC:3.5.1.86]
Other DBs
RHEA: 22879

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